A Correlation Between Proteinuria, Enzymuria And Kidney Histopathological Changes During Early Renal Damage Induced By Gentamlcln
This study was carried out to determine the correlation between urine proteins and urinary enzyme markers, aspartate aminotransferase (AST), yglutamyl transferase (y-GT) and alkaline phosphatase (ALP) and tissue histopathological changes during early renal damage induced by gentamicin. Fifty fiv...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2004
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/6580/1/FPV_2004_6.pdf |
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| Summary: | This study was carried out to determine the correlation between urine
proteins and urinary enzyme markers, aspartate aminotransferase (AST), yglutamyl
transferase (y-GT) and alkaline phosphatase (ALP) and tissue
histopathological changes during early renal damage induced by gentamicin.
Fifty five Sprague-Dawley rats, aged 7-8 weeks old were divided into two
groups. One group of ten rats served as controls and forty-five rats were
treated with gentamicin intraperitoneally to induce renal damage. Twenty four
hour urine samples were collected daily for 3 days. On the third day, blood
was withdrawn through cardiac puncture and kidney tissues from sacrificed
rats were taken for histopathological analysis. Urine proteins were separated
by sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDSPAGE).
Concentration of urine proteins, enzymes, serum urea nitrogen
(BUN) and creatinine were analysed using a chemistry analyser. The SDS-PAGE of urine proteins showed that proteinuria in gentamicin-treated
rats consisted primarily of protein with low molecular weight. The separated
urine proteins were mainly located in the molecular weight range of 10 to >
70 kDa. An extra protein band with molecular weight estimated to be
approximately 11 kDa was also detected in the gentamicin-treated group and
suggested to be an indicator of early renal disease. The electrophoretic
patterns of urine proteins also demonstrated that the type of proteins leaking
into urine had also provided some insight on the location and degree of renal
injury. High urine enzymes concentrations specifically ALP had also reflected
that the onset of injury was in the renal proximal tubular cells. This was
confirmed by renal tissue histopathological evidences. Each parameter
showed a high grading in gentamicin-treated group. Grade 3 cytoplasmic
vacuolization (31%), grade 3 tubular dilatation (33.3%) and grade 3 cell
detachment (33.3%) were the most prominent morphological changes in that
group. BUN and serum creatinine did not show any obvious difference
between the two groups. In conclusion, the elevated concentrations of low
molecular weight urine proteins and urine enzymes appeared to be useful
indicators for early renal damage as their elevation occurred before the BUN
and serum creatinine produced any change. |
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