Extraction and characterization of acetic acid-soluble collagen and papain-soluble collagen hydrolysate from poultry eggshell membrane
Collagens have unique biocompatible and biodegradable properties that are widely used in food, cosmetics, and biomedical applications. However, collagen derived from land-based animals pose problems as a risk to transmissible disease as Bovine Spongiform Encephalopathy (BSE) and footand- mouth d...
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| Format: | Thesis |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/66213/1/IPPH%202016%205%20IR.pdf |
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| Summary: | Collagens have unique biocompatible and biodegradable properties that are
widely used in food, cosmetics, and biomedical applications. However,
collagen derived from land-based animals pose problems as a risk to
transmissible disease as Bovine Spongiform Encephalopathy (BSE) and footand-
mouth diseases, the risk of autoimmune and allergic reaction in its
biomedical applications, and skepticism amongst faith-based niche market
particularly for Muslims and Jewish consumers. Collagen extracted from
eggshell membrane was thus proposed as an alternative. Extraction efficiency
and characterization of collagen of acetic acid-soluble collagen (ASC) and
papain-soluble collagen hydrolysates (PSCH) from eggshell membrane were
investigated. Studies were carried out under three variables (enzyme
concentration, temperature, and pH), it was found that the most efficient
conditions for ASC was at 15 °C, pH 3, with a yield of 0.23% on a dry weight
basis, while PSCH was at 15 °C, pH 3 and enzyme concentration of 300K U/g
of raw material with a yield of 0.55% on a dry weight basis. The isoelectric
points of ASC and PSCH were found to be at pH 6.61 and 6.89, respectively.
The FTIR spectra of both ASC and PSCH demonstrated similar absorption
bands to that of porcine. The maximum transition temperature of ASC and
entropy were observed at 61.84°C and 0.139 J/g, whilst PSCH was at 59.12°C
and 0.046 J/g, indicating such thermal profile was almost at the par of the
mammalian thermal profile. The color determination of ASC revealed that it
had bright intensity of lightness, low intensity of redness and yellowness, whilst
the colour determination of PSCH revealed that it had a slightly higher intensity
of redness. ASC in 0.5 M acetic acid had high solubility till pH 3 and then
remain relatively stable till pH 10, whilst PSCH in 0.5 M acetic acid had high
solubility till pH 4 then remain relatively stable to pH 10. For the effect of NaCl
concentration on collagen solubility, ASC demonstrated a distinguishable
decrease in solubility especially at concentrations above 2% and then
decreased slightly at above 4%, whilst PSCH demonstrated drastic decrease
at 3% and remain steady at above 4%. The odor patterns of ASC displays a low intensity, PSCH revealed that it had considerable intensity of odor which
require careful consideration in its application. |
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