Physical characteristics, morphology and protein profiles of salivary glands of two local edible nest swiftlet species
In the age of twenty centuries, edible bird nest (EBN) was highly contributed towards most of the Asian countries economy. However, as the EBN is constructed from saliva of the swiftlet, association between the nest and the salivary glands is still obscure. This is a fundamental study providin...
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my-upm-ir.667612019-01-31T04:35:12Z Physical characteristics, morphology and protein profiles of salivary glands of two local edible nest swiftlet species 2016-02 Ibrahim, Muhammad Marwan In the age of twenty centuries, edible bird nest (EBN) was highly contributed towards most of the Asian countries economy. However, as the EBN is constructed from saliva of the swiftlet, association between the nest and the salivary glands is still obscure. This is a fundamental study providing valuable information about the salivary glands of EBN swiftlets. The first objective of this study was to compare the morphological characteristics of Malaysia’s EBN swiftlets and examination of the histomorphology of the salivary glands. The second objective which is the main part of the study was to profile and identify the difference in protein spots from the salivary glands of EBN swiftlets. The last objective was to profile the glycoproteins of EBN swiftlets salivary gland using lectin based detection method. A total of 20 birds were caught in the Gomantong Cave, Sabah, out of which, 10 of it were A. fuciphagus and the balance were A. maximus. Both species were compared morphologically and it shows that A. maximus are larger in body size compared to A. fuciphagus. The sublingual gland on the other hand shows that, both species have morphologically similar including the size. The tissue was then stained with H&E stain and results show that both species generally have quite similar tissue structure. Combination of Alcian Blue-Periodic Acid Schiff shows both species are rich in mixture of acidic and neutral mucins secretion. However, A. fuciphagus has greater neutral mucin secretion compared in A. maximus. Based on relative sublingual glands weight, comparison between female A. fuciphagus and female A, maximus are the only one that shows significantly difference. Proteomics studies commenced with the protein quantification and A. fuciphagus male has the highest concentration of protein which is 8.675 μg/μL followed by A. fuciphagus female 6.875 μg/μL, while in male and female A. maximus consist of 5.743 and 6.117 μg/μL of proteins respectively. 2D gel was performed to obtain the salivary gland protein profiles and the differential proteins expressed were identified using LC/MS Q-ToF mass spectrometry analysis. Profiling of the glycoproteins shows most of the glycoproteins present are the N-link glycoproteins and conjugated with sialic acid. Swifts - Nests Morphology 2016-02 Thesis http://psasir.upm.edu.my/id/eprint/66761/ http://psasir.upm.edu.my/id/eprint/66761/1/IB%202016%2019%20IR.pdf text en public masters Universiti Putra Malaysia Swifts - Nests Morphology |
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Swifts - Nests Morphology |
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Swifts - Nests Morphology Ibrahim, Muhammad Marwan Physical characteristics, morphology and protein profiles of salivary glands of two local edible nest swiftlet species |
description |
In the age of twenty centuries, edible bird nest (EBN) was highly contributed towards
most of the Asian countries economy. However, as the EBN is constructed from
saliva of the swiftlet, association between the nest and the salivary glands is still
obscure. This is a fundamental study providing valuable information about the
salivary glands of EBN swiftlets. The first objective of this study was to compare the
morphological characteristics of Malaysia’s EBN swiftlets and examination of the
histomorphology of the salivary glands. The second objective which is the main part
of the study was to profile and identify the difference in protein spots from the
salivary glands of EBN swiftlets. The last objective was to profile the glycoproteins
of EBN swiftlets salivary gland using lectin based detection method. A total of 20
birds were caught in the Gomantong Cave, Sabah, out of which, 10 of it were A.
fuciphagus and the balance were A. maximus. Both species were compared
morphologically and it shows that A. maximus are larger in body size compared to A.
fuciphagus. The sublingual gland on the other hand shows that, both species have
morphologically similar including the size. The tissue was then stained with H&E
stain and results show that both species generally have quite similar tissue structure.
Combination of Alcian Blue-Periodic Acid Schiff shows both species are rich in
mixture of acidic and neutral mucins secretion. However, A. fuciphagus has greater
neutral mucin secretion compared in A. maximus. Based on relative sublingual glands
weight, comparison between female A. fuciphagus and female A, maximus are the
only one that shows significantly difference. Proteomics studies commenced with the
protein quantification and A. fuciphagus male has the highest concentration of
protein which is 8.675 μg/μL followed by A. fuciphagus female 6.875 μg/μL, while
in male and female A. maximus consist of 5.743 and 6.117 μg/μL of proteins
respectively. 2D gel was performed to obtain the salivary gland protein profiles and the differential proteins expressed were identified using LC/MS Q-ToF mass
spectrometry analysis. Profiling of the glycoproteins shows most of the
glycoproteins present are the N-link glycoproteins and conjugated with sialic acid. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Ibrahim, Muhammad Marwan |
author_facet |
Ibrahim, Muhammad Marwan |
author_sort |
Ibrahim, Muhammad Marwan |
title |
Physical characteristics, morphology and protein profiles of salivary glands of two local edible nest swiftlet species |
title_short |
Physical characteristics, morphology and protein profiles of salivary glands of two local edible nest swiftlet species |
title_full |
Physical characteristics, morphology and protein profiles of salivary glands of two local edible nest swiftlet species |
title_fullStr |
Physical characteristics, morphology and protein profiles of salivary glands of two local edible nest swiftlet species |
title_full_unstemmed |
Physical characteristics, morphology and protein profiles of salivary glands of two local edible nest swiftlet species |
title_sort |
physical characteristics, morphology and protein profiles of salivary glands of two local edible nest swiftlet species |
granting_institution |
Universiti Putra Malaysia |
publishDate |
2016 |
url |
http://psasir.upm.edu.my/id/eprint/66761/1/IB%202016%2019%20IR.pdf |
_version_ |
1747812403085574144 |