In Vitro evaluation of cockle shell-based calcium carbonate aragonite polymorph nanoparticle with surface functionalization for drug delivery applications
Drug delivery is a current biomedical application employing nano-sized particles. In line with current global interest, employing nature-based materials to construct delivery carriers has been highly preferable due to their environmental friendly, availability, low cost, and good natural miner...
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my-upm-ir.667632019-01-31T04:34:37Z In Vitro evaluation of cockle shell-based calcium carbonate aragonite polymorph nanoparticle with surface functionalization for drug delivery applications 2016-12 Mohd Abd Ghafar, Syairah Liyana Drug delivery is a current biomedical application employing nano-sized particles. In line with current global interest, employing nature-based materials to construct delivery carriers has been highly preferable due to their environmental friendly, availability, low cost, and good natural mineral purity. Cockle (Anadara granosa) shells was reported to contain comparable mineral compositions to vertebrates bone with high calcium carbon and no evident presence of heavy metal elements with good quality and pure calcium carbonate aragonite crystals. In order to meet stringent qualities of drug carrier for drug delivery applications, an improved synthesis method incorporated with surface functionalization was developed to produce nanoparticles with high homogeneity in size and shape. The study aimed at evaluating the physicochemical characteristics of surface functionalized cockle shell-based calcium carbonate aragonite nanoparticle and its potentials as delivery agent. Cockle shell micron-sized powder was converted into nano-sized particles through a mechanical stirring process in the presence of dodecyl dimethyl betaine (BS-12). The effect of BS- 12 surfactant on the surface property of cockle shell-based calcium carbonate aragonite nanoparticles was analyzed through pH evaluation, Fourier Transform Infrared (FTIR) and X-ray diffraction (XRD) analyzes. Transmission Electron Microscopy (TEM) and Field Emission Scanning Electron Microscopy (FESEM) demonstrated agglomeration of nanoparticles after the addition of surfactant. However, with calcium ion adsorption onto the surface of cockle shell-based calcium carbonate aragonite, the dispersion of the nanoparticles has improved as shown by the increase in zeta potential. Purification technique further enhanced the overall distribution of nanoparticles towards more refined size range of less than 100 nanometers, which is favorable for drug delivery applications. The purity of aragonite phase and chemical functionality were verified by FTIR and X-ray diffraction (XRD) analyzes. In vitro biological response on human fetal osteoblast (hFOB 1.19) cell line demonstrated that surface functionalization could decrease cytotoxicity. Surface functionalized cockle shell-based calcium carbonate aragonite nanocarrier showed better capacity to load drug molecules and was able to sustain incorporation of some drug molecules up to three days in vitro. Both the cockle shell-based nanocarrier samples were sensitive to pH changes as they released more drug compounds in the acidic environment of pH 6.4 than pH 7.4. This new delivery agent from cockle shells may provide an alternative source for calcium carbonate aragonite polymorph nanoparticles as an efficient drug carrier for therapeutic applications. Nanotechnology Cockles 2016-12 Thesis http://psasir.upm.edu.my/id/eprint/66763/ http://psasir.upm.edu.my/id/eprint/66763/1/IB%202016%2025%20IR.pdf text en public masters Universiti Putra Malaysia Nanotechnology Cockles |
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Nanotechnology Cockles Mohd Abd Ghafar, Syairah Liyana In Vitro evaluation of cockle shell-based calcium carbonate aragonite polymorph nanoparticle with surface functionalization for drug delivery applications |
description |
Drug delivery is a current biomedical application employing nano-sized particles. In
line with current global interest, employing nature-based materials to construct
delivery carriers has been highly preferable due to their environmental friendly,
availability, low cost, and good natural mineral purity. Cockle (Anadara granosa)
shells was reported to contain comparable mineral compositions to vertebrates bone
with high calcium carbon and no evident presence of heavy metal elements with good
quality and pure calcium carbonate aragonite crystals. In order to meet stringent
qualities of drug carrier for drug delivery applications, an improved synthesis method
incorporated with surface functionalization was developed to produce nanoparticles
with high homogeneity in size and shape. The study aimed at evaluating the
physicochemical characteristics of surface functionalized cockle shell-based calcium
carbonate aragonite nanoparticle and its potentials as delivery agent. Cockle shell
micron-sized powder was converted into nano-sized particles through a mechanical
stirring process in the presence of dodecyl dimethyl betaine (BS-12). The effect of BS-
12 surfactant on the surface property of cockle shell-based calcium carbonate
aragonite nanoparticles was analyzed through pH evaluation, Fourier Transform
Infrared (FTIR) and X-ray diffraction (XRD) analyzes. Transmission Electron
Microscopy (TEM) and Field Emission Scanning Electron Microscopy (FESEM)
demonstrated agglomeration of nanoparticles after the addition of surfactant.
However, with calcium ion adsorption onto the surface of cockle shell-based calcium
carbonate aragonite, the dispersion of the nanoparticles has improved as shown by the
increase in zeta potential. Purification technique further enhanced the overall
distribution of nanoparticles towards more refined size range of less than 100
nanometers, which is favorable for drug delivery applications. The purity of aragonite
phase and chemical functionality were verified by FTIR and X-ray diffraction (XRD)
analyzes. In vitro biological response on human fetal osteoblast (hFOB 1.19) cell line
demonstrated that surface functionalization could decrease cytotoxicity. Surface
functionalized cockle shell-based calcium carbonate aragonite nanocarrier showed better capacity to load drug molecules and was able to sustain incorporation of some
drug molecules up to three days in vitro. Both the cockle shell-based nanocarrier
samples were sensitive to pH changes as they released more drug compounds in the
acidic environment of pH 6.4 than pH 7.4. This new delivery agent from cockle shells
may provide an alternative source for calcium carbonate aragonite polymorph
nanoparticles as an efficient drug carrier for therapeutic applications. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Mohd Abd Ghafar, Syairah Liyana |
author_facet |
Mohd Abd Ghafar, Syairah Liyana |
author_sort |
Mohd Abd Ghafar, Syairah Liyana |
title |
In Vitro evaluation of cockle shell-based calcium carbonate aragonite polymorph nanoparticle with surface functionalization for drug delivery applications |
title_short |
In Vitro evaluation of cockle shell-based calcium carbonate aragonite polymorph nanoparticle with surface functionalization for drug delivery applications |
title_full |
In Vitro evaluation of cockle shell-based calcium carbonate aragonite polymorph nanoparticle with surface functionalization for drug delivery applications |
title_fullStr |
In Vitro evaluation of cockle shell-based calcium carbonate aragonite polymorph nanoparticle with surface functionalization for drug delivery applications |
title_full_unstemmed |
In Vitro evaluation of cockle shell-based calcium carbonate aragonite polymorph nanoparticle with surface functionalization for drug delivery applications |
title_sort |
in vitro evaluation of cockle shell-based calcium carbonate aragonite polymorph nanoparticle with surface functionalization for drug delivery applications |
granting_institution |
Universiti Putra Malaysia |
publishDate |
2016 |
url |
http://psasir.upm.edu.my/id/eprint/66763/1/IB%202016%2025%20IR.pdf |
_version_ |
1747812403330940928 |