Nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications

Earthworms are invertebrates that have been widely applied to environmental and land study, however, a new chapter opens opportunities of research in cosmetic and medicine. The objectives of the experiment were to evaluate the nutritional and antioxidant properties from the earthworms Eudrilus eugen...

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Main Author:	Azmi, Nurhazirah
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Cosmetics - Research Earthworms
Online Access:	http://psasir.upm.edu.my/id/eprint/67177/1/IPPH%202013%205%20IR.pdf
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spelling	my-upm-ir.671772019-02-21T06:34:29Z Nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications 2013-12 Azmi, Nurhazirah Earthworms are invertebrates that have been widely applied to environmental and land study, however, a new chapter opens opportunities of research in cosmetic and medicine. The objectives of the experiment were to evaluate the nutritional and antioxidant properties from the earthworms Eudrilus eugeniae, Eisenia fetida and Lumbricus rubellus and to examine the bioactivity properties of the earthworms extract in tyrosinase, matrix metalloproteinase-1, and elastase inhibitory activities for cosmeceutical applications. The analyzed components include moisture, available carbohydrates, total lipids, dietary fiber, crude protein, amino acids, ash, and minerals. The amino acids profile of the earthworm extracts was evaluated using High Performance Liquid Chromatography (HPLC). Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-Page) was used to determine and separate the protein based on size. The results showed E. fetida had slightly higher amount of protein and ash than L. rubellus and E. euginae. All the earthworm extracts showed high amounts of minerals ranging from 3-22% in calcium, potassium and phosphorus. All the extracts had the least amount of the amino acid cysteine, ranging from 0.17-0.21 mg/100 g protein, while the highest concentration is leucine, being 1.0-2.5 mg/100 g protein. Lysine and methionine, which were the limiting amino acids in most feedstuff, were present in all the extracts, ranging from 0.29-1.16 mg/100 g proteins and 0.3-0.57 mg/100 g protein, respectively. Molecular weights of earthworm extracts showed from 13-430 kDa under the optimal conditions of sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-Page). E. fetida exhibited wide molecular weight distribution compared to L. rubellus and E. eugeniae which consisted of 11, 10 and 6 prominent bands, respectively. In addition, E. fetida had the highest antioxidant activity (91.76%) using 2,2-diphenyl-1-picrylhydracyl (DPPH) assay compared to E. eugeniae and L. rubellus. At 25 mg/ml concentration, the antioxidant activity of E. fetida using DPPH assay was almost comparable to ascorbic acid (10mg/ml) which is usually used as antioxidant in most pharmaceutical products. All extracts demonstrated excellent inhibitory effects on tyrosinase. Extract from E. fetida gave the highest tyrosinase inhibition activity (80.12%) compared to L. rubellus (72.02%) and E. eugeniae (71.28%). However, their inhibition was still lower than the standard L-cysteine. In conclusion, this study provides nutritional compositional of earthworms as well as new findings on bioactivity properties having antioxidant, anti-tyrosinase and anti-aging properties. All the earthworms demonstrated valuable properties in antioxidant, anti-aging and whitening inhibition capacity despite the species. As such, it can be a potential candidate for application and utilization in cosmeceutical products. Cosmetics - Research Earthworms 2013-12 Thesis http://psasir.upm.edu.my/id/eprint/67177/ http://psasir.upm.edu.my/id/eprint/67177/1/IPPH%202013%205%20IR.pdf text en public masters Universiti Putra Malaysia Cosmetics - Research Earthworms
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Cosmetics - Research Earthworms
spellingShingle	Cosmetics - Research Earthworms Azmi, Nurhazirah Nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications
description	Earthworms are invertebrates that have been widely applied to environmental and land study, however, a new chapter opens opportunities of research in cosmetic and medicine. The objectives of the experiment were to evaluate the nutritional and antioxidant properties from the earthworms Eudrilus eugeniae, Eisenia fetida and Lumbricus rubellus and to examine the bioactivity properties of the earthworms extract in tyrosinase, matrix metalloproteinase-1, and elastase inhibitory activities for cosmeceutical applications. The analyzed components include moisture, available carbohydrates, total lipids, dietary fiber, crude protein, amino acids, ash, and minerals. The amino acids profile of the earthworm extracts was evaluated using High Performance Liquid Chromatography (HPLC). Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-Page) was used to determine and separate the protein based on size. The results showed E. fetida had slightly higher amount of protein and ash than L. rubellus and E. euginae. All the earthworm extracts showed high amounts of minerals ranging from 3-22% in calcium, potassium and phosphorus. All the extracts had the least amount of the amino acid cysteine, ranging from 0.17-0.21 mg/100 g protein, while the highest concentration is leucine, being 1.0-2.5 mg/100 g protein. Lysine and methionine, which were the limiting amino acids in most feedstuff, were present in all the extracts, ranging from 0.29-1.16 mg/100 g proteins and 0.3-0.57 mg/100 g protein, respectively. Molecular weights of earthworm extracts showed from 13-430 kDa under the optimal conditions of sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-Page). E. fetida exhibited wide molecular weight distribution compared to L. rubellus and E. eugeniae which consisted of 11, 10 and 6 prominent bands, respectively. In addition, E. fetida had the highest antioxidant activity (91.76%) using 2,2-diphenyl-1-picrylhydracyl (DPPH) assay compared to E. eugeniae and L. rubellus. At 25 mg/ml concentration, the antioxidant activity of E. fetida using DPPH assay was almost comparable to ascorbic acid (10mg/ml) which is usually used as antioxidant in most pharmaceutical products. All extracts demonstrated excellent inhibitory effects on tyrosinase. Extract from E. fetida gave the highest tyrosinase inhibition activity (80.12%) compared to L. rubellus (72.02%) and E. eugeniae (71.28%). However, their inhibition was still lower than the standard L-cysteine. In conclusion, this study provides nutritional compositional of earthworms as well as new findings on bioactivity properties having antioxidant, anti-tyrosinase and anti-aging properties. All the earthworms demonstrated valuable properties in antioxidant, anti-aging and whitening inhibition capacity despite the species. As such, it can be a potential candidate for application and utilization in cosmeceutical products.
format	Thesis
qualification_level	Master's degree
author	Azmi, Nurhazirah
author_facet	Azmi, Nurhazirah
author_sort	Azmi, Nurhazirah
title	Nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications
title_short	Nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications
title_full	Nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications
title_fullStr	Nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications
title_full_unstemmed	Nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications
title_sort	nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications
granting_institution	Universiti Putra Malaysia
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/67177/1/IPPH%202013%205%20IR.pdf
_version_	1747812448198459392

Nutritional and bioactivity properties of selected earthworm species for potential cosmeceutical applications

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