Application of gas chromatography-mass spectrometry and elemental analyzer-isotope ratio mass spectrometry techniques to distinguish lard from selected animal fats before and after chemical glycerolysis

A study was conducted to differentiate lard from selected animal fats namely chicken fat, beef fat and mutton fat, before and after chemical glycerolysis. It was carried out using Gas Chromatography Mass Spectrometry (GCMS) and Elemental Analyzer–Isotope Ratio Mass Spectrometry (EA-IRMS) techniques....

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Main Author:	Ahmad Nizar, Nina Naquiah
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Gas chromatography - Analysis Glycerin Lard
Online Access:	http://psasir.upm.edu.my/id/eprint/67236/1/IPPH%202013%203%20IR.pdf
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id	my-upm-ir.67236
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spelling	my-upm-ir.672362019-02-21T08:21:33Z Application of gas chromatography-mass spectrometry and elemental analyzer-isotope ratio mass spectrometry techniques to distinguish lard from selected animal fats before and after chemical glycerolysis 2013-07 Ahmad Nizar, Nina Naquiah A study was conducted to differentiate lard from selected animal fats namely chicken fat, beef fat and mutton fat, before and after chemical glycerolysis. It was carried out using Gas Chromatography Mass Spectrometry (GCMS) and Elemental Analyzer–Isotope Ratio Mass Spectrometry (EA-IRMS) techniques. The comparison of overall fatty acid data obtained by Gas Chromatography analysis before and after chemical glycerolysis showed that lard and chicken fats shared common characteristics by having palmitic, oleic and linoleic acids as major fatty acids. On the other hand, beef and mutton fats shared common characteristics by possessing palmitic, stearic and oleic acid as major fatty acids. Direct comparisons among the fatty acid data therefore may not be suitable for differentiation of animal fats. When the fatty acid distributional data of the animal fats was subjected to Principle Component Analysis (PCA), it was demonstrated that stearic, oleic and linoleic acids were the most discriminating parameters in the clustering of animal fats to four subclasses. The stable isotope analysis of lard and selected animal fats before chemical glycerolysis using EA-IRMS showed significant difference in the carbon isotope ratios (δ13C). The same finding was observed after chemical glycerolysis. This would be a good indicator in discrimination of lard, chicken, beef and mutton fats. The current finding leads to a more efficient method, to screen and ascertain the source of origin of fats used in food products. Gas chromatography - Analysis Glycerin Lard 2013-07 Thesis http://psasir.upm.edu.my/id/eprint/67236/ http://psasir.upm.edu.my/id/eprint/67236/1/IPPH%202013%203%20IR.pdf text en public masters Universiti Putra Malaysia Gas chromatography - Analysis Glycerin Lard
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Gas chromatography - Analysis Glycerin Lard
spellingShingle	Gas chromatography - Analysis Glycerin Lard Ahmad Nizar, Nina Naquiah Application of gas chromatography-mass spectrometry and elemental analyzer-isotope ratio mass spectrometry techniques to distinguish lard from selected animal fats before and after chemical glycerolysis
description	A study was conducted to differentiate lard from selected animal fats namely chicken fat, beef fat and mutton fat, before and after chemical glycerolysis. It was carried out using Gas Chromatography Mass Spectrometry (GCMS) and Elemental Analyzer–Isotope Ratio Mass Spectrometry (EA-IRMS) techniques. The comparison of overall fatty acid data obtained by Gas Chromatography analysis before and after chemical glycerolysis showed that lard and chicken fats shared common characteristics by having palmitic, oleic and linoleic acids as major fatty acids. On the other hand, beef and mutton fats shared common characteristics by possessing palmitic, stearic and oleic acid as major fatty acids. Direct comparisons among the fatty acid data therefore may not be suitable for differentiation of animal fats. When the fatty acid distributional data of the animal fats was subjected to Principle Component Analysis (PCA), it was demonstrated that stearic, oleic and linoleic acids were the most discriminating parameters in the clustering of animal fats to four subclasses. The stable isotope analysis of lard and selected animal fats before chemical glycerolysis using EA-IRMS showed significant difference in the carbon isotope ratios (δ13C). The same finding was observed after chemical glycerolysis. This would be a good indicator in discrimination of lard, chicken, beef and mutton fats. The current finding leads to a more efficient method, to screen and ascertain the source of origin of fats used in food products.
format	Thesis
qualification_level	Master's degree
author	Ahmad Nizar, Nina Naquiah
author_facet	Ahmad Nizar, Nina Naquiah
author_sort	Ahmad Nizar, Nina Naquiah
title	Application of gas chromatography-mass spectrometry and elemental analyzer-isotope ratio mass spectrometry techniques to distinguish lard from selected animal fats before and after chemical glycerolysis
title_short	Application of gas chromatography-mass spectrometry and elemental analyzer-isotope ratio mass spectrometry techniques to distinguish lard from selected animal fats before and after chemical glycerolysis
title_full	Application of gas chromatography-mass spectrometry and elemental analyzer-isotope ratio mass spectrometry techniques to distinguish lard from selected animal fats before and after chemical glycerolysis
title_fullStr	Application of gas chromatography-mass spectrometry and elemental analyzer-isotope ratio mass spectrometry techniques to distinguish lard from selected animal fats before and after chemical glycerolysis
title_full_unstemmed	Application of gas chromatography-mass spectrometry and elemental analyzer-isotope ratio mass spectrometry techniques to distinguish lard from selected animal fats before and after chemical glycerolysis
title_sort	application of gas chromatography-mass spectrometry and elemental analyzer-isotope ratio mass spectrometry techniques to distinguish lard from selected animal fats before and after chemical glycerolysis
granting_institution	Universiti Putra Malaysia
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/67236/1/IPPH%202013%203%20IR.pdf
_version_	1747812450672050176

Application of gas chromatography-mass spectrometry and elemental analyzer-isotope ratio mass spectrometry techniques to distinguish lard from selected animal fats before and after chemical glycerolysis

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