Development of protocol for in vitro regeneration in Abelmoscus esculentus (L.) Moench (okra) through shoot apical meristem, and nutrient and biochemical composition of its leaves and fruits

Okra is an important vegetable of valuable nutrient, good sources of vitamins, important antioxidants and medicinal compounds such as polyphenols and gossypol. The tissue culture was carried out by using shoot apical meristem while the nutritional and biochemical values of fresh okra leaves and frui...

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Main Author:	Chika, Nwachukwu Emmanuel
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Plant micropropagation
Online Access:	http://psasir.upm.edu.my/id/eprint/67488/1/FS%202013%2081%20IR.pdf
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id	my-upm-ir.67488
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spelling	my-upm-ir.674882019-03-14T02:37:48Z Development of protocol for in vitro regeneration in Abelmoscus esculentus (L.) Moench (okra) through shoot apical meristem, and nutrient and biochemical composition of its leaves and fruits 2013-07 Chika, Nwachukwu Emmanuel Okra is an important vegetable of valuable nutrient, good sources of vitamins, important antioxidants and medicinal compounds such as polyphenols and gossypol. The tissue culture was carried out by using shoot apical meristem while the nutritional and biochemical values of fresh okra leaves and fruits were evaluated. Meristematic tissues (0.4 -0.6mm) of okra seedlings were excised. Shoot apical meristems (SAM) were cultured in MS medium supplemented with a range of concentrations of NAA (0.1 mg/l 0.5mg/l, BAP (0.1mg/l, 2- 4.0mg/l), and in combination of NAA and BAP (0.1 mg/l – 1.0mg/l). This present study showed the highest shoot proliferations and formation from SAM on semi-solid MS medium cultured on 1.5mg/l BAP with four shoots per culture and best rooting on 1.0mg/l with an average of 4 roots per culture. The plantlets were transferred into pots containing soil for acclimatization and showed average of 82% survival. The proximate analysis was used to determine the percentage of protein, carbohydrate, ash, fats, fiber and moisture present in okra leaves and fruits. In addition, selected biochemical properties of okra leaves and fruits such as Chlorophyll a, b, xanthophylls and caretonoids, total phenolics and total flavonoids were measured spectrophotometrically. This study revealed the highest percentage of protein (4.82%) and ash (2.44%) in okra leaves while fruits contain highest percentage carbohydrate (11.17%), crude fiber (2.44%) and moisture (88.47%). Interestingly, this present study found that okra leaf contains higher chlorophyll, total phenolics and total flavonoid than okra fruit. Okra leaf showed highest chlorophyll a (23.66mg/0.1g), carotenoids (4.63mg/0.1g), Chlorophyll b (6.6mg/0.1g). Furthermore, the result also showed highest total phenolics (0.99mg/ 0.1g) and flavonoids (0.80mg/0.1g) in the leaf. This study has established standard protocol for in vitro regeneration of mucilaginous plant such as okra and okra leaf has shown to contain high nutritional and biochemical value compared with okra fruit. Plant micropropagation 2013-07 Thesis http://psasir.upm.edu.my/id/eprint/67488/ http://psasir.upm.edu.my/id/eprint/67488/1/FS%202013%2081%20IR.pdf text en public masters Universiti Putra Malaysia Plant micropropagation
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Plant micropropagation
spellingShingle	Plant micropropagation Chika, Nwachukwu Emmanuel Development of protocol for in vitro regeneration in Abelmoscus esculentus (L.) Moench (okra) through shoot apical meristem, and nutrient and biochemical composition of its leaves and fruits
description	Okra is an important vegetable of valuable nutrient, good sources of vitamins, important antioxidants and medicinal compounds such as polyphenols and gossypol. The tissue culture was carried out by using shoot apical meristem while the nutritional and biochemical values of fresh okra leaves and fruits were evaluated. Meristematic tissues (0.4 -0.6mm) of okra seedlings were excised. Shoot apical meristems (SAM) were cultured in MS medium supplemented with a range of concentrations of NAA (0.1 mg/l 0.5mg/l, BAP (0.1mg/l, 2- 4.0mg/l), and in combination of NAA and BAP (0.1 mg/l – 1.0mg/l). This present study showed the highest shoot proliferations and formation from SAM on semi-solid MS medium cultured on 1.5mg/l BAP with four shoots per culture and best rooting on 1.0mg/l with an average of 4 roots per culture. The plantlets were transferred into pots containing soil for acclimatization and showed average of 82% survival. The proximate analysis was used to determine the percentage of protein, carbohydrate, ash, fats, fiber and moisture present in okra leaves and fruits. In addition, selected biochemical properties of okra leaves and fruits such as Chlorophyll a, b, xanthophylls and caretonoids, total phenolics and total flavonoids were measured spectrophotometrically. This study revealed the highest percentage of protein (4.82%) and ash (2.44%) in okra leaves while fruits contain highest percentage carbohydrate (11.17%), crude fiber (2.44%) and moisture (88.47%). Interestingly, this present study found that okra leaf contains higher chlorophyll, total phenolics and total flavonoid than okra fruit. Okra leaf showed highest chlorophyll a (23.66mg/0.1g), carotenoids (4.63mg/0.1g), Chlorophyll b (6.6mg/0.1g). Furthermore, the result also showed highest total phenolics (0.99mg/ 0.1g) and flavonoids (0.80mg/0.1g) in the leaf. This study has established standard protocol for in vitro regeneration of mucilaginous plant such as okra and okra leaf has shown to contain high nutritional and biochemical value compared with okra fruit.
format	Thesis
qualification_level	Master's degree
author	Chika, Nwachukwu Emmanuel
author_facet	Chika, Nwachukwu Emmanuel
author_sort	Chika, Nwachukwu Emmanuel
title	Development of protocol for in vitro regeneration in Abelmoscus esculentus (L.) Moench (okra) through shoot apical meristem, and nutrient and biochemical composition of its leaves and fruits
title_short	Development of protocol for in vitro regeneration in Abelmoscus esculentus (L.) Moench (okra) through shoot apical meristem, and nutrient and biochemical composition of its leaves and fruits
title_full	Development of protocol for in vitro regeneration in Abelmoscus esculentus (L.) Moench (okra) through shoot apical meristem, and nutrient and biochemical composition of its leaves and fruits
title_fullStr	Development of protocol for in vitro regeneration in Abelmoscus esculentus (L.) Moench (okra) through shoot apical meristem, and nutrient and biochemical composition of its leaves and fruits
title_full_unstemmed	Development of protocol for in vitro regeneration in Abelmoscus esculentus (L.) Moench (okra) through shoot apical meristem, and nutrient and biochemical composition of its leaves and fruits
title_sort	development of protocol for in vitro regeneration in abelmoscus esculentus (l.) moench (okra) through shoot apical meristem, and nutrient and biochemical composition of its leaves and fruits
granting_institution	Universiti Putra Malaysia
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/67488/1/FS%202013%2081%20IR.pdf
_version_	1747812477184245760

Development of protocol for in vitro regeneration in Abelmoscus esculentus (L.) Moench (okra) through shoot apical meristem, and nutrient and biochemical composition of its leaves and fruits

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