Detection of viruses from patients hospitalized with lower respiratory tract infections in a public hospital, Malaysia

Lower respiratory tract infections (LRTI) are among the most common human infectious diseases worldwide and responsible for a considerable number of deaths among children, particularly in developing countries. Respiratory tract infections are caused by broad spectrum microbial agents, mostly viruses...

Full description

Saved in:

Bibliographic Details
Main Author:	Musa, Siti Noorhidayah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Respiratory Tract Infections Viruses
Online Access:	http://psasir.upm.edu.my/id/eprint/68588/1/FPSK%28M%29%202018%2016%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.68588
record_format	uketd_dc
spelling	my-upm-ir.685882019-05-21T06:37:53Z Detection of viruses from patients hospitalized with lower respiratory tract infections in a public hospital, Malaysia 2017-03 Musa, Siti Noorhidayah Lower respiratory tract infections (LRTI) are among the most common human infectious diseases worldwide and responsible for a considerable number of deaths among children, particularly in developing countries. Respiratory tract infections are caused by broad spectrum microbial agents, mostly viruses and some bacteria. The common of respiratory viruses includes influenza virus A and B, parainfluenza viruses, adenoviruses, respiratory syncytial virus (RSV), rhinoviruses, and coronaviruses. In Malaysia, studies on the prevalence of these viruses are still lacking of data presentation of the virus prevalence associated with LRTI. The purpose of this study was to detect the respiratory virus aetiology from patients in Sungai Buloh Hospital, Selangor who were hospitalized with lower respiratory tract infections. A total of 210 specimens and patient’s data were collected from patients hospitalized with LRTI. The specimens were collected from April 2013 until early January 2014 after routine immunofluorescence (IF) test in hospital. Samples subjected to viral nucleic acid extraction and PCR for adenovirus, bocavirus and RSV detection. Positive PCR samples were sequenced and phylogenetic trees were constructed. Sample then analyzed based on demographic and clinical data. PCR result revealed 18 samples that were positive with adenovirus which were 11 of HAdV-7, two HAdV-1, HAdV-2 and HAdV-4 respectively and one was HAdV-5 while two positive PCR samples were HBoV1. Hospital IF test reported presence of RSV, influenza A, adenovirus, parainfluenza 3 and influenza B. Virus incidence was observed higher in children compared to adult with noticeable symptoms observed were cough, fever and difficulty of breathing, while asthma was the common underlying disease presented. Main diagnoses of the LRTI were pneumonia and bronchiolitis. In summary, viruses contribute to the etiology of LRTI among hospitalized patients in Hospital Sungai Buloh, particularly RSV followed by HAdV, influenza A, parainfluenza 3, HBoV and influenza B. Respiratory Tract Infections Viruses Hospitals, Public - Malaysia 2017-03 Thesis http://psasir.upm.edu.my/id/eprint/68588/ http://psasir.upm.edu.my/id/eprint/68588/1/FPSK%28M%29%202018%2016%20IR.pdf text en public masters Universiti Putra Malaysia Respiratory Tract Infections Viruses Hospitals, Public - Malaysia
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Respiratory Tract Infections Viruses Respiratory Tract Infections
spellingShingle	Respiratory Tract Infections Viruses Respiratory Tract Infections Musa, Siti Noorhidayah Detection of viruses from patients hospitalized with lower respiratory tract infections in a public hospital, Malaysia
description	Lower respiratory tract infections (LRTI) are among the most common human infectious diseases worldwide and responsible for a considerable number of deaths among children, particularly in developing countries. Respiratory tract infections are caused by broad spectrum microbial agents, mostly viruses and some bacteria. The common of respiratory viruses includes influenza virus A and B, parainfluenza viruses, adenoviruses, respiratory syncytial virus (RSV), rhinoviruses, and coronaviruses. In Malaysia, studies on the prevalence of these viruses are still lacking of data presentation of the virus prevalence associated with LRTI. The purpose of this study was to detect the respiratory virus aetiology from patients in Sungai Buloh Hospital, Selangor who were hospitalized with lower respiratory tract infections. A total of 210 specimens and patient’s data were collected from patients hospitalized with LRTI. The specimens were collected from April 2013 until early January 2014 after routine immunofluorescence (IF) test in hospital. Samples subjected to viral nucleic acid extraction and PCR for adenovirus, bocavirus and RSV detection. Positive PCR samples were sequenced and phylogenetic trees were constructed. Sample then analyzed based on demographic and clinical data. PCR result revealed 18 samples that were positive with adenovirus which were 11 of HAdV-7, two HAdV-1, HAdV-2 and HAdV-4 respectively and one was HAdV-5 while two positive PCR samples were HBoV1. Hospital IF test reported presence of RSV, influenza A, adenovirus, parainfluenza 3 and influenza B. Virus incidence was observed higher in children compared to adult with noticeable symptoms observed were cough, fever and difficulty of breathing, while asthma was the common underlying disease presented. Main diagnoses of the LRTI were pneumonia and bronchiolitis. In summary, viruses contribute to the etiology of LRTI among hospitalized patients in Hospital Sungai Buloh, particularly RSV followed by HAdV, influenza A, parainfluenza 3, HBoV and influenza B.
format	Thesis
qualification_level	Master's degree
author	Musa, Siti Noorhidayah
author_facet	Musa, Siti Noorhidayah
author_sort	Musa, Siti Noorhidayah
title	Detection of viruses from patients hospitalized with lower respiratory tract infections in a public hospital, Malaysia
title_short	Detection of viruses from patients hospitalized with lower respiratory tract infections in a public hospital, Malaysia
title_full	Detection of viruses from patients hospitalized with lower respiratory tract infections in a public hospital, Malaysia
title_fullStr	Detection of viruses from patients hospitalized with lower respiratory tract infections in a public hospital, Malaysia
title_full_unstemmed	Detection of viruses from patients hospitalized with lower respiratory tract infections in a public hospital, Malaysia
title_sort	detection of viruses from patients hospitalized with lower respiratory tract infections in a public hospital, malaysia
granting_institution	Universiti Putra Malaysia
publishDate	2017
url	http://psasir.upm.edu.my/id/eprint/68588/1/FPSK%28M%29%202018%2016%20IR.pdf
_version_	1747812601515999232

Detection of viruses from patients hospitalized with lower respiratory tract infections in a public hospital, Malaysia

Similar Items