Optical biosensor based on immobilized copper sensitive operon repressor protein for detection of copper ions in water

Water pollution, from heavy metals, embodies a latent risk for both terrestrial and aquatic organisms. As such, continuous, simple and sensitive detection tools for toxic metals have been a great challenge of the existing methods used in monitoring of these metals. In this case, biosensors stand wor...

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Main Author:	Ismail, Hassan
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Biosensors Copper ions
Online Access:	http://psasir.upm.edu.my/id/eprint/68954/1/FBSB%202016%202%20-%20IR.pdf
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id	my-upm-ir.68954
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spelling	my-upm-ir.689542019-06-18T01:09:54Z Optical biosensor based on immobilized copper sensitive operon repressor protein for detection of copper ions in water 2016-05 Ismail, Hassan Water pollution, from heavy metals, embodies a latent risk for both terrestrial and aquatic organisms. As such, continuous, simple and sensitive detection tools for toxic metals have been a great challenge of the existing methods used in monitoring of these metals. In this case, biosensors stand worthwhile for constant monitoring of metals in polluted areas. Here a new biosensor based on a tapered Multi-Mode Fiber (MMF) coated with CsoRGZ protein from Geobacillus zalihae (CsoRGz) as a bioreceptor is presented. The optical property of the coated layer changes once it was subjected to copper, resulting in an increase, at the UV-region (240 nm), of the absorption of evanescent waves. This increase of absorption is proportional to the concentration of copper added. The biosensor displayed a continuous response over the range of 5 - 40 μM copper. The limit of detection and limit of quantification were 5 μM and 40 μM copper respectively, with the sensitivity of 0.045 μM-1 based on 20 μm sensor. The biosensor showed a fast response time of 19.8 s at room temperature and pH of 7.0. The biosensor retained its selectivity and did not respond to equivalent additions of cobalt (II) and Nickel (II). This work also revealed, for the first time, the prospect of remote, selective and sensitive monitoring of copper ions in water, from a distance of 50 m, where a continuous response in the range of 5 - 40 μM copper was obtained with no significant difference (p> 0.06) with the non-remote one. Biosensors Copper ions 2016-05 Thesis http://psasir.upm.edu.my/id/eprint/68954/ http://psasir.upm.edu.my/id/eprint/68954/1/FBSB%202016%202%20-%20IR.pdf text en public masters Universiti Putra Malaysia Biosensors Copper ions
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Biosensors Copper ions
spellingShingle	Biosensors Copper ions Ismail, Hassan Optical biosensor based on immobilized copper sensitive operon repressor protein for detection of copper ions in water
description	Water pollution, from heavy metals, embodies a latent risk for both terrestrial and aquatic organisms. As such, continuous, simple and sensitive detection tools for toxic metals have been a great challenge of the existing methods used in monitoring of these metals. In this case, biosensors stand worthwhile for constant monitoring of metals in polluted areas. Here a new biosensor based on a tapered Multi-Mode Fiber (MMF) coated with CsoRGZ protein from Geobacillus zalihae (CsoRGz) as a bioreceptor is presented. The optical property of the coated layer changes once it was subjected to copper, resulting in an increase, at the UV-region (240 nm), of the absorption of evanescent waves. This increase of absorption is proportional to the concentration of copper added. The biosensor displayed a continuous response over the range of 5 - 40 μM copper. The limit of detection and limit of quantification were 5 μM and 40 μM copper respectively, with the sensitivity of 0.045 μM-1 based on 20 μm sensor. The biosensor showed a fast response time of 19.8 s at room temperature and pH of 7.0. The biosensor retained its selectivity and did not respond to equivalent additions of cobalt (II) and Nickel (II). This work also revealed, for the first time, the prospect of remote, selective and sensitive monitoring of copper ions in water, from a distance of 50 m, where a continuous response in the range of 5 - 40 μM copper was obtained with no significant difference (p> 0.06) with the non-remote one.
format	Thesis
qualification_level	Master's degree
author	Ismail, Hassan
author_facet	Ismail, Hassan
author_sort	Ismail, Hassan
title	Optical biosensor based on immobilized copper sensitive operon repressor protein for detection of copper ions in water
title_short	Optical biosensor based on immobilized copper sensitive operon repressor protein for detection of copper ions in water
title_full	Optical biosensor based on immobilized copper sensitive operon repressor protein for detection of copper ions in water
title_fullStr	Optical biosensor based on immobilized copper sensitive operon repressor protein for detection of copper ions in water
title_full_unstemmed	Optical biosensor based on immobilized copper sensitive operon repressor protein for detection of copper ions in water
title_sort	optical biosensor based on immobilized copper sensitive operon repressor protein for detection of copper ions in water
granting_institution	Universiti Putra Malaysia
publishDate	2016
url	http://psasir.upm.edu.my/id/eprint/68954/1/FBSB%202016%202%20-%20IR.pdf
_version_	1747812652067848192

Optical biosensor based on immobilized copper sensitive operon repressor protein for detection of copper ions in water

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