Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent

Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent

Lipase catalysed synthesis of xylose caproate ester was performed by condensation of xylose, an aldopentose, and caproic acid in organic solvents. A dual-solvent system containing dimethylsulfoxide (DMSO) and acetone (1:10, v/v) was used to determine the optimal conditions for the reaction. Differen...

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Main Author: Hamidon, Noor Fazrieyana
Format: Thesis
Language:English
Published: 2016
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Online Access:http://psasir.upm.edu.my/id/eprint/69292/1/FS%202016%2076%20UPMIR.pdf
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spelling my-upm-ir.692922021-09-08T00:42:13Z Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent 2016-11 Hamidon, Noor Fazrieyana Lipase catalysed synthesis of xylose caproate ester was performed by condensation of xylose, an aldopentose, and caproic acid in organic solvents. A dual-solvent system containing dimethylsulfoxide (DMSO) and acetone (1:10, v/v) was used to determine the optimal conditions for the reaction. Different reaction parameters (solvent system, reaction time, substrate molar ratio and the amount of enzyme loaded) were studied. The highest conversion rate (64%) was obtained within 24 hours with the optimal conditions of 16% (w/v) Novozym 435 and a molar ratio of xylose to caproic acid of 1:4 (mmol). Xylose caproate ester was purified and characterized by thin layer chromatography (TLC), high performance liquid chromatography (HPLC), fourier transform infrared spectroscopy (FTIR), gas chromatography flame ionization (GCFID), gas chromatography mass spectrometry (GCMS) and nuclear magnetic resonance (NMR). The pure product existed as a waxy solid form with bright yellow color. The melting point, hydrophilic lipophipic value (HLB), and solubility of the product was evaluated. 2016-11 Thesis http://psasir.upm.edu.my/id/eprint/69292/ http://psasir.upm.edu.my/id/eprint/69292/1/FS%202016%2076%20UPMIR.pdf text en public masters Universiti Putra Malaysia Abd Malek, Emilia
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
advisor Abd Malek, Emilia
topic


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Hamidon, Noor Fazrieyana
Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent
description Lipase catalysed synthesis of xylose caproate ester was performed by condensation of xylose, an aldopentose, and caproic acid in organic solvents. A dual-solvent system containing dimethylsulfoxide (DMSO) and acetone (1:10, v/v) was used to determine the optimal conditions for the reaction. Different reaction parameters (solvent system, reaction time, substrate molar ratio and the amount of enzyme loaded) were studied. The highest conversion rate (64%) was obtained within 24 hours with the optimal conditions of 16% (w/v) Novozym 435 and a molar ratio of xylose to caproic acid of 1:4 (mmol). Xylose caproate ester was purified and characterized by thin layer chromatography (TLC), high performance liquid chromatography (HPLC), fourier transform infrared spectroscopy (FTIR), gas chromatography flame ionization (GCFID), gas chromatography mass spectrometry (GCMS) and nuclear magnetic resonance (NMR). The pure product existed as a waxy solid form with bright yellow color. The melting point, hydrophilic lipophipic value (HLB), and solubility of the product was evaluated.
format Thesis
qualification_level Master's degree
author Hamidon, Noor Fazrieyana
author_facet Hamidon, Noor Fazrieyana
author_sort Hamidon, Noor Fazrieyana
title Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent
title_short Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent
title_full Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent
title_fullStr Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent
title_full_unstemmed Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent
title_sort optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent
granting_institution Universiti Putra Malaysia
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/69292/1/FS%202016%2076%20UPMIR.pdf
_version_ 1747812680076361728

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