Evaluation of different post-harvest treatments on Malaysian mistletoe (Scurrula ferruginea (Jack) Dans.) leaves extracts yield, antioxidant and antimicrobial performance
Scurrula ferruginea is hemiparasitic mistletoes from the family Loranthaceae that widely distributed in tropical regions and has been used traditionally as medicinal plants in Southeast Asian countries. In earliest studies, the plant is known to have antioxidant and antimicrobial activities. Howe...
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my-upm-ir.692932019-09-20T02:16:10Z Evaluation of different post-harvest treatments on Malaysian mistletoe (Scurrula ferruginea (Jack) Dans.) leaves extracts yield, antioxidant and antimicrobial performance 2016-11 Justine, Vanielie Terrence Scurrula ferruginea is hemiparasitic mistletoes from the family Loranthaceae that widely distributed in tropical regions and has been used traditionally as medicinal plants in Southeast Asian countries. In earliest studies, the plant is known to have antioxidant and antimicrobial activities. However, the best drying methods towards the preservation of phenolic compounds of the plant as well as a suitable solvent for extracting the antioxidants and antimicrobial activity have not been optimized. In this study, the determination of the best drying methods (air and oven) and extraction solvents (aqueous, organic and aqueous organic) on phenolic compounds, antioxidant and antimicrobial activity were carried out. Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) were used to determine the phenolic compound, while antioxidant activity was evaluated by measuring the scavenging effect on 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical and Ferric Reducing Antioxidant Power (FRAP). Antimicrobial activity of the extracts was tested against 2 pathogenic bacteria (Staphylococcus aureus S261 and Escherichia coli E57) and 2 pathogenic fungi species (Candida albicans C205 and Trichophyton rubrum T62) using Disc Diffusion Method, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) respectively. The optimal conditions for S. ferruginea were oven drying, using 80% methanol solvent with values of 2.72 mg/10g DW (extract yield), 168.18 mg GAE/1g DW (TPC), 25.03 mg QE/1g DW (TFC), 27.86 mg TE/1g DW (FRAP), 94.0% (DPPH) and 19.32 μg/mL (IC50). The result of this research shows a potent antioxidant activity in S. ferruginea leaves extracts as compared to standard antioxidants (Ascorbic acid and Trolox). A correlation study of the extracts revealed significant positive correlations (P < 0.05) between extraction yields, phenolic compounds and antioxidant properties. The extracts exhibited optimum antimicrobial activities on both Staphylococcus aureus S261 and Escherichia coli E57 with a zone of inhibition ranging from 7.98 to 9.71 mm and 450 to 900 μg/mL (MIC and MBC). However, the extracts show no inhibitory effects on C. albicans C205 and T. rubrum T62. Present results suggested that post-harvest preparations such as drying method (oven drying) and extractions solvents (80% methanol) variability play a crucial role in enhancing the efficiency and efficacy of plant samples. The findings obtained in this study shows that S. ferruginea leaves are highly potential source of antioxidant and antimicrobial discovery that leads for new nutraceutical. Mistletoes Medicinal plants 2016-11 Thesis http://psasir.upm.edu.my/id/eprint/69293/ http://psasir.upm.edu.my/id/eprint/69293/1/FS%202016%2080%20IR.pdf text en public masters Universiti Putra Malaysia Mistletoes Medicinal plants |
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Mistletoes Medicinal plants Justine, Vanielie Terrence Evaluation of different post-harvest treatments on Malaysian mistletoe (Scurrula ferruginea (Jack) Dans.) leaves extracts yield, antioxidant and antimicrobial performance |
description |
Scurrula ferruginea is hemiparasitic mistletoes from the family Loranthaceae that
widely distributed in tropical regions and has been used traditionally as medicinal
plants in Southeast Asian countries. In earliest studies, the plant is known to have
antioxidant and antimicrobial activities. However, the best drying methods towards the
preservation of phenolic compounds of the plant as well as a suitable solvent for
extracting the antioxidants and antimicrobial activity have not been optimized. In this
study, the determination of the best drying methods (air and oven) and extraction
solvents (aqueous, organic and aqueous organic) on phenolic compounds, antioxidant
and antimicrobial activity were carried out. Total Phenolic Content (TPC) and Total
Flavonoid Content (TFC) were used to determine the phenolic compound, while
antioxidant activity was evaluated by measuring the scavenging effect on 2,2-
diphenyl-1-picrylhydrazyl (DPPH) radical and Ferric Reducing Antioxidant Power
(FRAP). Antimicrobial activity of the extracts was tested against 2 pathogenic bacteria
(Staphylococcus aureus S261 and Escherichia coli E57) and 2 pathogenic fungi species
(Candida albicans C205 and Trichophyton rubrum T62) using Disc Diffusion Method,
Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration
(MBC) and Minimum Fungicidal Concentration (MFC) respectively. The optimal
conditions for S. ferruginea were oven drying, using 80% methanol solvent with values
of 2.72 mg/10g DW (extract yield), 168.18 mg GAE/1g DW (TPC), 25.03 mg QE/1g
DW (TFC), 27.86 mg TE/1g DW (FRAP), 94.0% (DPPH) and 19.32 μg/mL (IC50). The
result of this research shows a potent antioxidant activity in S. ferruginea leaves
extracts as compared to standard antioxidants (Ascorbic acid and Trolox). A correlation
study of the extracts revealed significant positive correlations (P < 0.05) between
extraction yields, phenolic compounds and antioxidant properties. The extracts
exhibited optimum antimicrobial activities on both Staphylococcus aureus S261 and
Escherichia coli E57 with a zone of inhibition ranging from 7.98 to 9.71 mm and 450
to 900 μg/mL (MIC and MBC). However, the extracts show no inhibitory effects on C.
albicans C205 and T. rubrum T62. Present results suggested that post-harvest
preparations such as drying method (oven drying) and extractions solvents (80%
methanol) variability play a crucial role in enhancing the efficiency and efficacy of plant samples. The findings obtained in this study shows that S. ferruginea leaves are
highly potential source of antioxidant and antimicrobial discovery that leads for new
nutraceutical. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Justine, Vanielie Terrence |
author_facet |
Justine, Vanielie Terrence |
author_sort |
Justine, Vanielie Terrence |
title |
Evaluation of different post-harvest treatments on Malaysian mistletoe (Scurrula ferruginea (Jack) Dans.) leaves extracts yield, antioxidant and antimicrobial performance |
title_short |
Evaluation of different post-harvest treatments on Malaysian mistletoe (Scurrula ferruginea (Jack) Dans.) leaves extracts yield, antioxidant and antimicrobial performance |
title_full |
Evaluation of different post-harvest treatments on Malaysian mistletoe (Scurrula ferruginea (Jack) Dans.) leaves extracts yield, antioxidant and antimicrobial performance |
title_fullStr |
Evaluation of different post-harvest treatments on Malaysian mistletoe (Scurrula ferruginea (Jack) Dans.) leaves extracts yield, antioxidant and antimicrobial performance |
title_full_unstemmed |
Evaluation of different post-harvest treatments on Malaysian mistletoe (Scurrula ferruginea (Jack) Dans.) leaves extracts yield, antioxidant and antimicrobial performance |
title_sort |
evaluation of different post-harvest treatments on malaysian mistletoe (scurrula ferruginea (jack) dans.) leaves extracts yield, antioxidant and antimicrobial performance |
granting_institution |
Universiti Putra Malaysia |
publishDate |
2016 |
url |
http://psasir.upm.edu.my/id/eprint/69293/1/FS%202016%2080%20IR.pdf |
_version_ |
1747812680324874240 |