Mechanisms of Antitumour Activity of 3,19-(2 Bromobenzylidene) Andrographolide (SRJ09)

To date, most of the clinical cytotoxic anticancer drugs target all rapidly dividing cells and are non-selective in their mechanism of action by disrupting essential components that are crucial to both malignant and normal cells. Hence, the search for more effective and selective anticancer drugs...

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Main Author:	Lim, Siang Hui
Format:	Thesis
Language:	English English
Published:	2007
Subjects:	Anticarcinogenic Agents
Online Access:	http://psasir.upm.edu.my/id/eprint/6965/1/FPSK%28M%29_2007_14.pdf
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id	my-upm-ir.6965
record_format	uketd_dc
spelling	my-upm-ir.69652023-11-21T07:04:49Z Mechanisms of Antitumour Activity of 3,19-(2 Bromobenzylidene) Andrographolide (SRJ09) 2007 Lim, Siang Hui To date, most of the clinical cytotoxic anticancer drugs target all rapidly dividing cells and are non-selective in their mechanism of action by disrupting essential components that are crucial to both malignant and normal cells. Hence, the search for more effective and selective anticancer drugs is currently being researched actively involving the various entities of the drug discovery programme. We at UPM have shown that andrographolide (AGP), a compound isolated from a local herb, Andrographis paniculata, to have anticancer activity in vitro and in vivo. In order to improve the antitumour properties of AGP, semi-synthetic derivatives of this compound were synthesised in our laboratory, with the aim of identifying the most promising anticancer compound among the AGP derivatives and to elucidate the mechanism(s) of action of the compound. The in vitro antitumour study showed that 3,19-(2-bromobenzylidene)andrographolide (SR09) displayed better antitumour activity when compared with AGP and other derivatives namely 3,19-(2- chlorobenzylidene)andrographolide (SRJ11) and 3,19-(3-chloro-4- fluorobenzylidene) andrographolide (SRJ23). The antitumour activity of AGP, SRJ09, SRJ11 and SRJ23 was shown to be not compromised by P-glycoprotein activities in MES-SA Dx5 multidrug resistant cell line. The time-course study revealed SRJ09 had a rapid acting interval compared with AGP. SRJ09 was previously shown to induce G1-phase cell cycle arrest and in this study the effect was shown attributed to increased of p21 (CDK inhibitor) expression without affecting the expression of cyclin D1. Apoptosis was the main mode of cell death induced by SRJ09 and was p53 and bcl-2 independent, which might suggest that SRJ09 act through the extrinsic apoptotic pathway. A simple pharmacokinetic study was performed in Balb/c for the purpose of dose selection for in vivo study revealed that SRJ09 had a relatively short half-life but was able to reach in vitro cytotoxic concentration range. In a subsequent in vivo antitumour study, SRJ09 delayed quadruple tumour growth by 4 day in HCT-116 colon cancer xenografted mice treated with 400 mg/kg SRJ09 (q4d×3) when compared with control. In conclusion, SRJ09 have been proven as a lead anticancer agent given to its ability to induce in vitro cell cycle arrest and apoptosis and to have in vivo antitumour activity. Therefore, further studies in improving the anticancer properties of SRJ09 by chemical modification will be advantageous. Anticarcinogenic Agents 2007 Thesis http://psasir.upm.edu.my/id/eprint/6965/ http://psasir.upm.edu.my/id/eprint/6965/1/FPSK%28M%29_2007_14.pdf text en public masters Universiti Putra Malaysia Anticarcinogenic Agents Medicine and Health Sciences Stanslas, Johnson English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Stanslas, Johnson
topic	Anticarcinogenic Agents
spellingShingle	Anticarcinogenic Agents Lim, Siang Hui Mechanisms of Antitumour Activity of 3,19-(2 Bromobenzylidene) Andrographolide (SRJ09)
description	To date, most of the clinical cytotoxic anticancer drugs target all rapidly dividing cells and are non-selective in their mechanism of action by disrupting essential components that are crucial to both malignant and normal cells. Hence, the search for more effective and selective anticancer drugs is currently being researched actively involving the various entities of the drug discovery programme. We at UPM have shown that andrographolide (AGP), a compound isolated from a local herb, Andrographis paniculata, to have anticancer activity in vitro and in vivo. In order to improve the antitumour properties of AGP, semi-synthetic derivatives of this compound were synthesised in our laboratory, with the aim of identifying the most promising anticancer compound among the AGP derivatives and to elucidate the mechanism(s) of action of the compound. The in vitro antitumour study showed that 3,19-(2-bromobenzylidene)andrographolide (SR09) displayed better antitumour activity when compared with AGP and other derivatives namely 3,19-(2- chlorobenzylidene)andrographolide (SRJ11) and 3,19-(3-chloro-4- fluorobenzylidene) andrographolide (SRJ23). The antitumour activity of AGP, SRJ09, SRJ11 and SRJ23 was shown to be not compromised by P-glycoprotein activities in MES-SA Dx5 multidrug resistant cell line. The time-course study revealed SRJ09 had a rapid acting interval compared with AGP. SRJ09 was previously shown to induce G1-phase cell cycle arrest and in this study the effect was shown attributed to increased of p21 (CDK inhibitor) expression without affecting the expression of cyclin D1. Apoptosis was the main mode of cell death induced by SRJ09 and was p53 and bcl-2 independent, which might suggest that SRJ09 act through the extrinsic apoptotic pathway. A simple pharmacokinetic study was performed in Balb/c for the purpose of dose selection for in vivo study revealed that SRJ09 had a relatively short half-life but was able to reach in vitro cytotoxic concentration range. In a subsequent in vivo antitumour study, SRJ09 delayed quadruple tumour growth by 4 day in HCT-116 colon cancer xenografted mice treated with 400 mg/kg SRJ09 (q4d×3) when compared with control. In conclusion, SRJ09 have been proven as a lead anticancer agent given to its ability to induce in vitro cell cycle arrest and apoptosis and to have in vivo antitumour activity. Therefore, further studies in improving the anticancer properties of SRJ09 by chemical modification will be advantageous.
format	Thesis
qualification_level	Master's degree
author	Lim, Siang Hui
author_facet	Lim, Siang Hui
author_sort	Lim, Siang Hui
title	Mechanisms of Antitumour Activity of 3,19-(2 Bromobenzylidene) Andrographolide (SRJ09)
title_short	Mechanisms of Antitumour Activity of 3,19-(2 Bromobenzylidene) Andrographolide (SRJ09)
title_full	Mechanisms of Antitumour Activity of 3,19-(2 Bromobenzylidene) Andrographolide (SRJ09)
title_fullStr	Mechanisms of Antitumour Activity of 3,19-(2 Bromobenzylidene) Andrographolide (SRJ09)
title_full_unstemmed	Mechanisms of Antitumour Activity of 3,19-(2 Bromobenzylidene) Andrographolide (SRJ09)
title_sort	mechanisms of antitumour activity of 3,19-(2 bromobenzylidene) andrographolide (srj09)
granting_institution	Universiti Putra Malaysia
granting_department	Medicine and Health Sciences
publishDate	2007
url	http://psasir.upm.edu.my/id/eprint/6965/1/FPSK%28M%29_2007_14.pdf
_version_	1794018695172849664

Mechanisms of Antitumour Activity of 3,19-(2 Bromobenzylidene) Andrographolide (SRJ09)

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