Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines

Crystal nanoparticle is a new system with potential as a therapeutic agent in the treatment of diseases. The objectives of this study are to synthesize, characterize and determine the anticancer cell effects of iron-manganese and iron-doped sulfated zirconia nanoparticles. In this study the iron-...

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Main Author:	Khlaif, Mohamed Qasim
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Cancer cells Nanocrystals
Online Access:	http://psasir.upm.edu.my/id/eprint/69706/1/IB%202016%204%20IR.pdf
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spelling	my-upm-ir.697062019-09-20T02:59:23Z Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines 2016-04 Khlaif, Mohamed Qasim Crystal nanoparticle is a new system with potential as a therapeutic agent in the treatment of diseases. The objectives of this study are to synthesize, characterize and determine the anticancer cell effects of iron-manganese and iron-doped sulfated zirconia nanoparticles. In this study the iron-manganese- and iron-doped sulfated zirconia nanoparticles were prepared by hydrothermal impregnation method followed by calcination. The characterization of both nanoparticles were carried out using X-ray diffraction (XRD), thermal gravimetric analysis (TGA), fourier transform infrared spectroscopy (FT-IR), Brunner-Emmett-Teller (BET) surface area measurements, Xray fluorescence (XRF), X-ray photoelectron spectroscopy, zeta potential (ZSP) measurement, and transmission electron microscopy (TEM). The cytotoxicity of the nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay on human breast adenocarcinoma (MDA-MB-231), colorectal adenocarcinoma (HT29), and hepatocellular carcinoma (HepG2) cell lines, and two normal human cell lines, the Chang liver and human umbilical vein endothelial cells (HUVEC) cell lines. The results showed that the iron-manganese- and iron-doped sulfated zirconia nanoparticles were of average size 12.7 and 32.0 nm, respectively and zeta potential of 15.0 and 0.206 mV, respectively. These nanoparticles tend to aggregate in solution. The iron-manganese- and iron-doped sulfated zirconia nanoparticles are highly toxic to the MDA-MB-231 and HepG2 cells, respectively, showing dramatic morphological changes suggesting loss of cell viability. The nanoparticles are comparatively less toxic to the HT29 cells compared to the other cancer cell lines. The study suggests that the anticancer effects of iron-manganese- and iron-doped sulfated zirconia nanoparticles implicate caspase-3, 8 and -9 in their anticancer cells activities. The findings from the study highlight the potential of ironmanganese- and iron-doped sulfated zirconia nanoparticles as therapeutic agents in the treatment of cancers, while showing lesser effect on normal cells. Cancer cells Nanocrystals 2016-04 Thesis http://psasir.upm.edu.my/id/eprint/69706/ http://psasir.upm.edu.my/id/eprint/69706/1/IB%202016%204%20IR.pdf text en public masters Universiti Putra Malaysia Cancer cells Nanocrystals
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Cancer cells Nanocrystals
spellingShingle	Cancer cells Nanocrystals Khlaif, Mohamed Qasim Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
description	Crystal nanoparticle is a new system with potential as a therapeutic agent in the treatment of diseases. The objectives of this study are to synthesize, characterize and determine the anticancer cell effects of iron-manganese and iron-doped sulfated zirconia nanoparticles. In this study the iron-manganese- and iron-doped sulfated zirconia nanoparticles were prepared by hydrothermal impregnation method followed by calcination. The characterization of both nanoparticles were carried out using X-ray diffraction (XRD), thermal gravimetric analysis (TGA), fourier transform infrared spectroscopy (FT-IR), Brunner-Emmett-Teller (BET) surface area measurements, Xray fluorescence (XRF), X-ray photoelectron spectroscopy, zeta potential (ZSP) measurement, and transmission electron microscopy (TEM). The cytotoxicity of the nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay on human breast adenocarcinoma (MDA-MB-231), colorectal adenocarcinoma (HT29), and hepatocellular carcinoma (HepG2) cell lines, and two normal human cell lines, the Chang liver and human umbilical vein endothelial cells (HUVEC) cell lines. The results showed that the iron-manganese- and iron-doped sulfated zirconia nanoparticles were of average size 12.7 and 32.0 nm, respectively and zeta potential of 15.0 and 0.206 mV, respectively. These nanoparticles tend to aggregate in solution. The iron-manganese- and iron-doped sulfated zirconia nanoparticles are highly toxic to the MDA-MB-231 and HepG2 cells, respectively, showing dramatic morphological changes suggesting loss of cell viability. The nanoparticles are comparatively less toxic to the HT29 cells compared to the other cancer cell lines. The study suggests that the anticancer effects of iron-manganese- and iron-doped sulfated zirconia nanoparticles implicate caspase-3, 8 and -9 in their anticancer cells activities. The findings from the study highlight the potential of ironmanganese- and iron-doped sulfated zirconia nanoparticles as therapeutic agents in the treatment of cancers, while showing lesser effect on normal cells.
format	Thesis
qualification_level	Master's degree
author	Khlaif, Mohamed Qasim
author_facet	Khlaif, Mohamed Qasim
author_sort	Khlaif, Mohamed Qasim
title	Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
title_short	Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
title_full	Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
title_fullStr	Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
title_full_unstemmed	Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
title_sort	physicochemical characterization and in vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
granting_institution	Universiti Putra Malaysia
publishDate	2016
url	http://psasir.upm.edu.my/id/eprint/69706/1/IB%202016%204%20IR.pdf
_version_	1747812720649961472

Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines

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