Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia

Twelve species of Malay traditional vegetables were screened for antioxidant and nitric oxide (NO) inhibitory activities. Ferric thiocyanate (FTC), thiobarbituric acid (TBA) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) methods were used for the antioxidant activity measurements, and th...

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Main Author:	Abas, Faridah
Format:	Thesis
Language:	English English
Published:	2005
Subjects:	Curcuma Biological diversity
Online Access:	http://psasir.upm.edu.my/id/eprint/7/1/1548938_t_IB_2005_2.pdf
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spelling	my-upm-ir.72020-01-21T08:51:17Z Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia 2005-02 Abas, Faridah Twelve species of Malay traditional vegetables were screened for antioxidant and nitric oxide (NO) inhibitory activities. Ferric thiocyanate (FTC), thiobarbituric acid (TBA) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) methods were used for the antioxidant activity measurements, and the Griess assay on elicited murine peritoneal macrophages was used to assess NO inhibitory activity of the extracts. Melicope ptelefolia, Cosmos caudatus, and Curcuma mangga were selected for further study, based on the results of their biological activity evaluations. Characterization of the NO inhibitory fractions of the Melicope ptelefolia using on-line high performance liquid chromatography (HPLC)-diode array detector (DAD)-mass spectrometry (MS), identified seven main constituents. The compounds were identified as kokusaginine (1), either kokusagine (2a) or 5- methoxymaculine (2b), 3-prenyl-2,4, 6-trihydroxyacetophenone (3), 3 (4), 3-geranylgeranyl- 2,4,6-trihydroxyaceto-phenone (5), 3-[4-O-(3,7-dimethyl-2, 6-octadienyl)phenyl]-2-propenoic acid (6),and 3- farnesylgeranyl-2,4,6-trihydroxyacetophenone (7). Phytochemical investigation of the methanolic extract of Cosmos caudatus led to the isolation of four known compounds namely, quercetin 3-O-β- arabinofuranoside (17), quercetin 3-O-α-rhamnoside (18), quercetin 3-O-β-glucoside (19), and quercetin (20). All four compounds isolated from C. caudatus showed strong antioxidant activity. The activity was in the order of 20 > 17 > 18 > 19 > α-tocopherol (standard). Curcuma Biological diversity 2005-02 Thesis http://psasir.upm.edu.my/id/eprint/7/ http://psasir.upm.edu.my/id/eprint/7/1/1548938_t_IB_2005_2.pdf application/pdf en public doctoral Universiti Putra Malaysia Curcuma Biological diversity Institute of Bioscience Lajis, Md Nordin English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Lajis, Md Nordin
topic	Curcuma Biological diversity
spellingShingle	Curcuma Biological diversity Abas, Faridah Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
description	Twelve species of Malay traditional vegetables were screened for antioxidant and nitric oxide (NO) inhibitory activities. Ferric thiocyanate (FTC), thiobarbituric acid (TBA) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) methods were used for the antioxidant activity measurements, and the Griess assay on elicited murine peritoneal macrophages was used to assess NO inhibitory activity of the extracts. Melicope ptelefolia, Cosmos caudatus, and Curcuma mangga were selected for further study, based on the results of their biological activity evaluations. Characterization of the NO inhibitory fractions of the Melicope ptelefolia using on-line high performance liquid chromatography (HPLC)-diode array detector (DAD)-mass spectrometry (MS), identified seven main constituents. The compounds were identified as kokusaginine (1), either kokusagine (2a) or 5- methoxymaculine (2b), 3-prenyl-2,4, 6-trihydroxyacetophenone (3), 3 (4), 3-geranylgeranyl- 2,4,6-trihydroxyaceto-phenone (5), 3-[4-O-(3,7-dimethyl-2, 6-octadienyl)phenyl]-2-propenoic acid (6),and 3- farnesylgeranyl-2,4,6-trihydroxyacetophenone (7). Phytochemical investigation of the methanolic extract of Cosmos caudatus led to the isolation of four known compounds namely, quercetin 3-O-β- arabinofuranoside (17), quercetin 3-O-α-rhamnoside (18), quercetin 3-O-β-glucoside (19), and quercetin (20). All four compounds isolated from C. caudatus showed strong antioxidant activity. The activity was in the order of 20 > 17 > 18 > 19 > α-tocopherol (standard).
format	Thesis
qualification_level	Doctorate
author	Abas, Faridah
author_facet	Abas, Faridah
author_sort	Abas, Faridah
title	Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
title_short	Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
title_full	Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
title_fullStr	Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
title_full_unstemmed	Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
title_sort	phytochemical and biological activity studies of cosmos caudatus and curcuma mangga and the online characterization of bioactive fractions from melicope ptelefolia
granting_institution	Universiti Putra Malaysia
granting_department	Institute of Bioscience
publishDate	2005
url	http://psasir.upm.edu.my/id/eprint/7/1/1548938_t_IB_2005_2.pdf
_version_	1747810161669439488

Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia

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