Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia

Twelve species of Malay traditional vegetables were screened for antioxidant and nitric oxide (NO) inhibitory activities. Ferric thiocyanate (FTC), thiobarbituric acid (TBA) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) methods were used for the antioxidant activity measurements, and th...

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Main Author: Abas, Faridah
Format: Thesis
Language:English
English
Published: 2005
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/7/1/1548938_t_IB_2005_2.pdf
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spelling my-upm-ir.72020-01-21T08:51:17Z Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia 2005-02 Abas, Faridah Twelve species of Malay traditional vegetables were screened for antioxidant and nitric oxide (NO) inhibitory activities. Ferric thiocyanate (FTC), thiobarbituric acid (TBA) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) methods were used for the antioxidant activity measurements, and the Griess assay on elicited murine peritoneal macrophages was used to assess NO inhibitory activity of the extracts. Melicope ptelefolia, Cosmos caudatus, and Curcuma mangga were selected for further study, based on the results of their biological activity evaluations. Characterization of the NO inhibitory fractions of the Melicope ptelefolia using on-line high performance liquid chromatography (HPLC)-diode array detector (DAD)-mass spectrometry (MS), identified seven main constituents. The compounds were identified as kokusaginine (1), either kokusagine (2a) or 5- methoxymaculine (2b), 3-prenyl-2,4, 6-trihydroxyacetophenone (3), 3 (4), 3-geranylgeranyl- 2,4,6-trihydroxyaceto-phenone (5), 3-[4-O-(3,7-dimethyl-2, 6-octadienyl)phenyl]-2-propenoic acid (6),and 3- farnesylgeranyl-2,4,6-trihydroxyacetophenone (7). Phytochemical investigation of the methanolic extract of Cosmos caudatus led to the isolation of four known compounds namely, quercetin 3-O-β- arabinofuranoside (17), quercetin 3-O-α-rhamnoside (18), quercetin 3-O-β-glucoside (19), and quercetin (20). All four compounds isolated from C. caudatus showed strong antioxidant activity. The activity was in the order of 20 > 17 > 18 > 19 > α-tocopherol (standard). Curcuma Biological diversity 2005-02 Thesis http://psasir.upm.edu.my/id/eprint/7/ http://psasir.upm.edu.my/id/eprint/7/1/1548938_t_IB_2005_2.pdf application/pdf en public doctoral Universiti Putra Malaysia Curcuma Biological diversity Institute of Bioscience Lajis, Md Nordin English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
advisor Lajis, Md Nordin
topic Curcuma
Biological diversity

spellingShingle Curcuma
Biological diversity

Abas, Faridah
Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
description Twelve species of Malay traditional vegetables were screened for antioxidant and nitric oxide (NO) inhibitory activities. Ferric thiocyanate (FTC), thiobarbituric acid (TBA) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) methods were used for the antioxidant activity measurements, and the Griess assay on elicited murine peritoneal macrophages was used to assess NO inhibitory activity of the extracts. Melicope ptelefolia, Cosmos caudatus, and Curcuma mangga were selected for further study, based on the results of their biological activity evaluations. Characterization of the NO inhibitory fractions of the Melicope ptelefolia using on-line high performance liquid chromatography (HPLC)-diode array detector (DAD)-mass spectrometry (MS), identified seven main constituents. The compounds were identified as kokusaginine (1), either kokusagine (2a) or 5- methoxymaculine (2b), 3-prenyl-2,4, 6-trihydroxyacetophenone (3), 3 (4), 3-geranylgeranyl- 2,4,6-trihydroxyaceto-phenone (5), 3-[4-O-(3,7-dimethyl-2, 6-octadienyl)phenyl]-2-propenoic acid (6),and 3- farnesylgeranyl-2,4,6-trihydroxyacetophenone (7). Phytochemical investigation of the methanolic extract of Cosmos caudatus led to the isolation of four known compounds namely, quercetin 3-O-β- arabinofuranoside (17), quercetin 3-O-α-rhamnoside (18), quercetin 3-O-β-glucoside (19), and quercetin (20). All four compounds isolated from C. caudatus showed strong antioxidant activity. The activity was in the order of 20 > 17 > 18 > 19 > α-tocopherol (standard).
format Thesis
qualification_level Doctorate
author Abas, Faridah
author_facet Abas, Faridah
author_sort Abas, Faridah
title Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
title_short Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
title_full Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
title_fullStr Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
title_full_unstemmed Phytochemical and Biological Activity Studies of Cosmos Caudatus and Curcuma Mangga and the Online Characterization of Bioactive Fractions from Melicope ptelefolia
title_sort phytochemical and biological activity studies of cosmos caudatus and curcuma mangga and the online characterization of bioactive fractions from melicope ptelefolia
granting_institution Universiti Putra Malaysia
granting_department Institute of Bioscience
publishDate 2005
url http://psasir.upm.edu.my/id/eprint/7/1/1548938_t_IB_2005_2.pdf
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