Comparative proteomic analysis of Ganoderma species during in vitro interaction with oil palm root

Basal Stem Rot (BSR) in oil palm caused by Ganoderma spp. is a deadly disease affecting oil palm plantation yield and global cooking oil supply. A pathogenic species, Ganoderma boninense is claimed as the main causal agent of BSR while Ganoderma tornatum is regarded as non-pathogenic and unable t...

Full description

Saved in:

Bibliographic Details
Main Author:	Said Hussin, Siti Nahdatul Isnaini
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Ganoderma diseases of plants Ganoderma Oil palm - Diseases and pests
Online Access:	http://psasir.upm.edu.my/id/eprint/70304/1/FBSB%202017%2031%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.70304
record_format	uketd_dc
spelling	my-upm-ir.703042019-08-29T01:28:35Z Comparative proteomic analysis of Ganoderma species during in vitro interaction with oil palm root 2017-05 Said Hussin, Siti Nahdatul Isnaini Basal Stem Rot (BSR) in oil palm caused by Ganoderma spp. is a deadly disease affecting oil palm plantation yield and global cooking oil supply. A pathogenic species, Ganoderma boninense is claimed as the main causal agent of BSR while Ganoderma tornatum is regarded as non-pathogenic and unable to infect living palms. Insufficient information on the infection mechanism and immature early detection strategy of the pathogen are among the disease control limitations. The existing molecular studies on the oil palm-Ganoderma interaction mainly focused on the response of the plant towards the fungus infection while the information on the pathogen responses is still a scarce. Therefore, in this study, response of the fungus at the infective stage during interaction with oil palm at the molecular level was investigated. An optimized protein extraction protocol for 2-Dimensional Electrophoresis (2-DE) gel analysis of Ganoderma spp. was developed and a comparative proteomic analysis were conducted to investigate the changes in the dikaryotic mycelial protein expression of the pathogenic G. boninense and non-pathogenic G. tornatum during in vitro interaction with oil palm root. The phenol/ammonium acetate in methanol was shown to be the most effective protein extraction method for 2-DE proteomic studies of Ganoderma spp. mycelia. Scanning Electron Microscope (SEM) images obtained confirmed the hyphae attachment and colonisation of both species on the oil palm root surface after 72 h of inoculation. Comparative proteomic analysis showed that the mycelial proteins from oil palm root exhibited different expression profiles when compared to the mycelia grown on Potato Dextrose Agar (PDA). Proteins differentially expressed in both species may have either direct or indirect link to virulence and pathogenicity, metabolism, growth and maintenance of both Ganoderma species. During the interaction, proteins with potential contribution to fungal pathogenicity such as enolase, alpha-aminoadipate reductase, carboxypeptidase, dienelactone hydrolase, glutamine synthetase (GS) and guanine nucleotide binding proteins (G proteins) were upregulated in G. boninense while bZIP protein, triose-phosphate-isomerase, redoxin and peroxiredoxin were mutually expressed in both species. Identification of these proteins during the interaction with oil palm roots may provide fundamental information for further investigation on specific roles of the identified proteins towards Ganoderma infection mechanism and facilitate selection of potential markers for early detection of BSR in the future. Ganoderma diseases of plants Ganoderma Oil palm - Diseases and pests 2017-05 Thesis http://psasir.upm.edu.my/id/eprint/70304/ http://psasir.upm.edu.my/id/eprint/70304/1/FBSB%202017%2031%20IR.pdf text en public masters Universiti Putra Malaysia Ganoderma diseases of plants Ganoderma Oil palm - Diseases and pests
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Ganoderma diseases of plants Ganoderma Oil palm - Diseases and pests
spellingShingle	Ganoderma diseases of plants Ganoderma Oil palm - Diseases and pests Said Hussin, Siti Nahdatul Isnaini Comparative proteomic analysis of Ganoderma species during in vitro interaction with oil palm root
description	Basal Stem Rot (BSR) in oil palm caused by Ganoderma spp. is a deadly disease affecting oil palm plantation yield and global cooking oil supply. A pathogenic species, Ganoderma boninense is claimed as the main causal agent of BSR while Ganoderma tornatum is regarded as non-pathogenic and unable to infect living palms. Insufficient information on the infection mechanism and immature early detection strategy of the pathogen are among the disease control limitations. The existing molecular studies on the oil palm-Ganoderma interaction mainly focused on the response of the plant towards the fungus infection while the information on the pathogen responses is still a scarce. Therefore, in this study, response of the fungus at the infective stage during interaction with oil palm at the molecular level was investigated. An optimized protein extraction protocol for 2-Dimensional Electrophoresis (2-DE) gel analysis of Ganoderma spp. was developed and a comparative proteomic analysis were conducted to investigate the changes in the dikaryotic mycelial protein expression of the pathogenic G. boninense and non-pathogenic G. tornatum during in vitro interaction with oil palm root. The phenol/ammonium acetate in methanol was shown to be the most effective protein extraction method for 2-DE proteomic studies of Ganoderma spp. mycelia. Scanning Electron Microscope (SEM) images obtained confirmed the hyphae attachment and colonisation of both species on the oil palm root surface after 72 h of inoculation. Comparative proteomic analysis showed that the mycelial proteins from oil palm root exhibited different expression profiles when compared to the mycelia grown on Potato Dextrose Agar (PDA). Proteins differentially expressed in both species may have either direct or indirect link to virulence and pathogenicity, metabolism, growth and maintenance of both Ganoderma species. During the interaction, proteins with potential contribution to fungal pathogenicity such as enolase, alpha-aminoadipate reductase, carboxypeptidase, dienelactone hydrolase, glutamine synthetase (GS) and guanine nucleotide binding proteins (G proteins) were upregulated in G. boninense while bZIP protein, triose-phosphate-isomerase, redoxin and peroxiredoxin were mutually expressed in both species. Identification of these proteins during the interaction with oil palm roots may provide fundamental information for further investigation on specific roles of the identified proteins towards Ganoderma infection mechanism and facilitate selection of potential markers for early detection of BSR in the future.
format	Thesis
qualification_level	Master's degree
author	Said Hussin, Siti Nahdatul Isnaini
author_facet	Said Hussin, Siti Nahdatul Isnaini
author_sort	Said Hussin, Siti Nahdatul Isnaini
title	Comparative proteomic analysis of Ganoderma species during in vitro interaction with oil palm root
title_short	Comparative proteomic analysis of Ganoderma species during in vitro interaction with oil palm root
title_full	Comparative proteomic analysis of Ganoderma species during in vitro interaction with oil palm root
title_fullStr	Comparative proteomic analysis of Ganoderma species during in vitro interaction with oil palm root
title_full_unstemmed	Comparative proteomic analysis of Ganoderma species during in vitro interaction with oil palm root
title_sort	comparative proteomic analysis of ganoderma species during in vitro interaction with oil palm root
granting_institution	Universiti Putra Malaysia
publishDate	2017
url	http://psasir.upm.edu.my/id/eprint/70304/1/FBSB%202017%2031%20IR.pdf
_version_	1747812807892533248

Comparative proteomic analysis of Ganoderma species during in vitro interaction with oil palm root

Similar Items