Identification of heat tolerant genotypes of chili pepper based on cell membrane thermostability and expression of heat shock protein genes
Terminal heat stress leads to change in the various parameters, such as heat shock protein genes and cell membrane thermostability, in chili pepper and is an important component of thermotolerance capacity. Experiments were carried out at Agrotechnology Unit, Agro Gene Bank and the Plant Physiolo...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/70321/1/FP%202014%2065%20-%20IR.pdf |
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| Summary: | Terminal heat stress leads to change in the various parameters, such as heat shock
protein genes and cell membrane thermostability, in chili pepper and is an important
component of thermotolerance capacity. Experiments were carried out at
Agrotechnology Unit, Agro Gene Bank and the Plant Physiology Laboratory,
Institute of Tropical Agriculture, Universiti Putra Malaysia to study the mechanisms
for heat tolerance in chili pepper genotypes. To assess these mechanisms, 36
genotypes were evaluated for morpho-physiological characters, cellular membrane
thermostability (CMT) and for HSP70 gene expression. Significant (p <0.05)
variation was observed among the genotypes for all the characters with genotypes
AVPP9905 (1144.3 g) and Kulai (818.1 g) recording the highest yield per plant
except chlorophyll content and days to flowering. The plants from selected chili
pepper genotypes for CMT and HSP70 gene experiments were grown in
experimental plant growth chamber for 4-8 weeks after sowing. The mean value of
CMT indicates in most of the genotypes that membrane integrity was not damaged
by the high temperature treatment (50ºC), with a mean value of 64.64%. The
genotypes were classified based on heat tolerant (>60%), moderately tolerant (30%-
60%) and susceptible (<30%). Genotypes AVPP0702, AVPP0116 and AVPP9905
recorded the highest CMTs, at 89.27%, 89.27% and 85.10% respectively. AVPP9703
and AVPP0002 gave CMTs of 15.87% and 18.43%, respectively which might
indicate their sensitivity to heat stress. HSP70 gene was identified and found
differentially expressed under heat stress condition. Under heat stress, significantly
increased levels of the HSP70 genes were detected after 2 hours temperature
treatment at 42°C, which indicated these genes are quickly and sharply induced by
heat shock. This was found especially true for all genotypes which were significantly
up-regulated by more than 36.9, 7.10, 3.87 and 3 fold, for AVPP0702, AVPP0116,
AVPP0002 and AVPP9703, respectively. Variety Kulai was found to be significantly
down regulated under heat stress. Genotypes AVPP0702, AVPP9905 and AVPP0116
could be considered as heat tolerant genotypes while Kulai and AVPP9703 as heat
sensitive genotypes from this study. The result further proves that CMT and HSP70
are key components of the defense mechanism against heat stress in chili pepper. |
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