Biological control of Pyricularia oryzae, using fungal metabolites

Rice blast caused by fungus Pyricularia oryzea is one of the most devastating among rice diseases. The disease has been classified as one of the most serious diseases that influence the production of rice since rice fields are seriously damaged, thereby affecting yield and quality. To decrease the u...

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Main Author:	Idan, Ali Abdulameer
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Rice - Diseases and pests - Biological control Pyricularia oryzae
Online Access:	http://psasir.upm.edu.my/id/eprint/70352/1/FP%202017%2025%20IR.pdf
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id	my-upm-ir.70352
record_format	uketd_dc
spelling	my-upm-ir.703522019-09-20T02:51:15Z Biological control of Pyricularia oryzae, using fungal metabolites 2017-10 Idan, Ali Abdulameer Rice blast caused by fungus Pyricularia oryzea is one of the most devastating among rice diseases. The disease has been classified as one of the most serious diseases that influence the production of rice since rice fields are seriously damaged, thereby affecting yield and quality. To decrease the use of pesticides, biological control is considered as an alternative sustainable control. Aspergillus spp. has been widely found to be useful in biotechnology as a bio control agent. They have a high ability in the production of secondary metabolites. Aspergillus spp. was obtained from the rice field soil located in Selangor, Tanjung Karang, Peninsular Malaysia. Isolates UPMZ01 and UPMZ02 were screened against blast pathogen by applying dual culture technique. The antagonism of isolate UPMZ01 was (81.326%) while UPMZ02 gave (70.9%) of inhibition percentage against P. oryzae. Culture filtrate was also used to screen the inhibitory effect against the pathogen. The results revealed that isolate UPMZ01 gave the optimum inhibitory percentage of (100%) at all concentrations of fungal secondary metabolites at 14 days. Based on ITS sequencing, the isolate UPMZ01 was identified as Aspergillus niger and UPMZ02 as Aspergillus flavus. The environmental factors such as pH and temperature influenced the production of secondary metabolites. The optimum condition for A. niger to produce efficient antifungal metabolites that gave (100%) PIGR against blast pathogen was at pH 5.0 and 21 to 29ºC. The secondary metabolites compounds were identified by gas chromatography-mass spectrometry (GC-MS). Fifteen compounds were recognized as major compounds. The identified compounds were Oleic Acid, n-Hexadecanoic acid, Hexose, Glycerol, Stearic acid, Tetradecanoic acid, Dodecanoic acid and 5-Hydrxoymethylfurfural. The Liquid chromatography-mass spectrophotometry (LC-MS/MS) analysis revealed four compounds, Succinic acid, Al21 anion, Diphenylarsine and N-Acetylaspartic acid. However, the culture filtrate of A. niger UPMZ01 may contribute to control rice blast pathogen. Rice - Diseases and pests - Biological control Pyricularia oryzae 2017-10 Thesis http://psasir.upm.edu.my/id/eprint/70352/ http://psasir.upm.edu.my/id/eprint/70352/1/FP%202017%2025%20IR.pdf text en public masters Universiti Putra Malaysia Rice - Diseases and pests - Biological control Pyricularia oryzae
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Rice - Diseases and pests - Biological control Pyricularia oryzae
spellingShingle	Rice - Diseases and pests - Biological control Pyricularia oryzae Idan, Ali Abdulameer Biological control of Pyricularia oryzae, using fungal metabolites
description	Rice blast caused by fungus Pyricularia oryzea is one of the most devastating among rice diseases. The disease has been classified as one of the most serious diseases that influence the production of rice since rice fields are seriously damaged, thereby affecting yield and quality. To decrease the use of pesticides, biological control is considered as an alternative sustainable control. Aspergillus spp. has been widely found to be useful in biotechnology as a bio control agent. They have a high ability in the production of secondary metabolites. Aspergillus spp. was obtained from the rice field soil located in Selangor, Tanjung Karang, Peninsular Malaysia. Isolates UPMZ01 and UPMZ02 were screened against blast pathogen by applying dual culture technique. The antagonism of isolate UPMZ01 was (81.326%) while UPMZ02 gave (70.9%) of inhibition percentage against P. oryzae. Culture filtrate was also used to screen the inhibitory effect against the pathogen. The results revealed that isolate UPMZ01 gave the optimum inhibitory percentage of (100%) at all concentrations of fungal secondary metabolites at 14 days. Based on ITS sequencing, the isolate UPMZ01 was identified as Aspergillus niger and UPMZ02 as Aspergillus flavus. The environmental factors such as pH and temperature influenced the production of secondary metabolites. The optimum condition for A. niger to produce efficient antifungal metabolites that gave (100%) PIGR against blast pathogen was at pH 5.0 and 21 to 29ºC. The secondary metabolites compounds were identified by gas chromatography-mass spectrometry (GC-MS). Fifteen compounds were recognized as major compounds. The identified compounds were Oleic Acid, n-Hexadecanoic acid, Hexose, Glycerol, Stearic acid, Tetradecanoic acid, Dodecanoic acid and 5-Hydrxoymethylfurfural. The Liquid chromatography-mass spectrophotometry (LC-MS/MS) analysis revealed four compounds, Succinic acid, Al21 anion, Diphenylarsine and N-Acetylaspartic acid. However, the culture filtrate of A. niger UPMZ01 may contribute to control rice blast pathogen.
format	Thesis
qualification_level	Master's degree
author	Idan, Ali Abdulameer
author_facet	Idan, Ali Abdulameer
author_sort	Idan, Ali Abdulameer
title	Biological control of Pyricularia oryzae, using fungal metabolites
title_short	Biological control of Pyricularia oryzae, using fungal metabolites
title_full	Biological control of Pyricularia oryzae, using fungal metabolites
title_fullStr	Biological control of Pyricularia oryzae, using fungal metabolites
title_full_unstemmed	Biological control of Pyricularia oryzae, using fungal metabolites
title_sort	biological control of pyricularia oryzae, using fungal metabolites
granting_institution	Universiti Putra Malaysia
publishDate	2017
url	http://psasir.upm.edu.my/id/eprint/70352/1/FP%202017%2025%20IR.pdf
_version_	1747812819702644736

Biological control of Pyricularia oryzae, using fungal metabolites

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