Potential of stem cell therapy during dengue infection in mice

Dengue is one of the most common arthropod-borne infections in the world which has been the major concern to the governments and World Health Organization (WHO). The virus causes over 390 million infections annually. Individual infected with dengue virus are suffering from several clinical symptoms...

Full description

Saved in:

Bibliographic Details
Main Author:	Maideensa Syed Gulam, Sakinah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Dengue - therapy Stem Cells
Online Access:	http://psasir.upm.edu.my/id/eprint/70972/1/FPSK%28M%29%202017%2014%20-%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.70972
record_format	uketd_dc
spelling	my-upm-ir.709722019-09-10T06:09:14Z Potential of stem cell therapy during dengue infection in mice 2017-05 Maideensa Syed Gulam, Sakinah Dengue is one of the most common arthropod-borne infections in the world which has been the major concern to the governments and World Health Organization (WHO). The virus causes over 390 million infections annually. Individual infected with dengue virus are suffering from several clinical symptoms including leukopenia, thrombocytopenia and hemorrhagic manifestations. Stem cell based therapy has been widely used for many disorders. The advance in regenerative therapy promises the alternative therapy for infectious diseases. Researchers reported about the stem cells treatment for infections in relation to HIV, malaria and tuberculosis. The success of these reports had laid a positive path to work in current objective, to investigate the potential of stem cells and progenitor cells with the growth factor infusion in protecting and repairing the injuries in dengue infected mice. Eight week old male BALB/c mice were divided into three groups; Control group: mice injected with eagle minimum essential media (EMEM) and phosphate buffer saline (PBS); Group 1: mice infected with DV-2 (G1); Group 2: mice infected with DV-2 and treated with hematopoietic stem cells (HSCs), endothelial progenitor cells (EPCs) and growth factor (GF) (G2). Mice in G1 and G2 were inoculated with DV-2. At day 2 of post infection, mice in G2 were infused stem cells (HSCs, EPCs and GF) intraveneously. Blood was collected from all groups at 5th, 10th, 15th, and 21st d.p.i for hematological assay, biochemical assay, and sacrificed for histopathological analysis and viral clearance analysis. Dengue infection in G1 results in thrombocytopenia, leucopenia and lymphocytopenia by 21st d.p.i, with low hemoglobin level and red blood cells count. Additionally, the biochemical assays (AST & ALT) increased showing significant correlation with the histopathological damages in liver. Moreover, blood vessel histopathology revealed injury correlating to the blood profile and all the damages was persistent till 21st d.p.i. The DV-2 infection with stem cell treatment group (G2) exhibits protective effect in blood profile, liver, blood vessel histopathology and viral clearance with the recovery initiated from 10th d.p.i. In conclusion, stem cell could be a new potential therapy not only to recover the altered blood profile, severe organ structural injury with its protective effect, but also completely clear the viral particle in order to help in normal body functioning. Dengue - therapy Stem Cells 2017-05 Thesis http://psasir.upm.edu.my/id/eprint/70972/ http://psasir.upm.edu.my/id/eprint/70972/1/FPSK%28M%29%202017%2014%20-%20IR.pdf text en public masters Universiti Putra Malaysia Dengue - therapy Stem Cells
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Dengue - therapy Stem Cells
spellingShingle	Dengue - therapy Stem Cells Maideensa Syed Gulam, Sakinah Potential of stem cell therapy during dengue infection in mice
description	Dengue is one of the most common arthropod-borne infections in the world which has been the major concern to the governments and World Health Organization (WHO). The virus causes over 390 million infections annually. Individual infected with dengue virus are suffering from several clinical symptoms including leukopenia, thrombocytopenia and hemorrhagic manifestations. Stem cell based therapy has been widely used for many disorders. The advance in regenerative therapy promises the alternative therapy for infectious diseases. Researchers reported about the stem cells treatment for infections in relation to HIV, malaria and tuberculosis. The success of these reports had laid a positive path to work in current objective, to investigate the potential of stem cells and progenitor cells with the growth factor infusion in protecting and repairing the injuries in dengue infected mice. Eight week old male BALB/c mice were divided into three groups; Control group: mice injected with eagle minimum essential media (EMEM) and phosphate buffer saline (PBS); Group 1: mice infected with DV-2 (G1); Group 2: mice infected with DV-2 and treated with hematopoietic stem cells (HSCs), endothelial progenitor cells (EPCs) and growth factor (GF) (G2). Mice in G1 and G2 were inoculated with DV-2. At day 2 of post infection, mice in G2 were infused stem cells (HSCs, EPCs and GF) intraveneously. Blood was collected from all groups at 5th, 10th, 15th, and 21st d.p.i for hematological assay, biochemical assay, and sacrificed for histopathological analysis and viral clearance analysis. Dengue infection in G1 results in thrombocytopenia, leucopenia and lymphocytopenia by 21st d.p.i, with low hemoglobin level and red blood cells count. Additionally, the biochemical assays (AST & ALT) increased showing significant correlation with the histopathological damages in liver. Moreover, blood vessel histopathology revealed injury correlating to the blood profile and all the damages was persistent till 21st d.p.i. The DV-2 infection with stem cell treatment group (G2) exhibits protective effect in blood profile, liver, blood vessel histopathology and viral clearance with the recovery initiated from 10th d.p.i. In conclusion, stem cell could be a new potential therapy not only to recover the altered blood profile, severe organ structural injury with its protective effect, but also completely clear the viral particle in order to help in normal body functioning.
format	Thesis
qualification_level	Master's degree
author	Maideensa Syed Gulam, Sakinah
author_facet	Maideensa Syed Gulam, Sakinah
author_sort	Maideensa Syed Gulam, Sakinah
title	Potential of stem cell therapy during dengue infection in mice
title_short	Potential of stem cell therapy during dengue infection in mice
title_full	Potential of stem cell therapy during dengue infection in mice
title_fullStr	Potential of stem cell therapy during dengue infection in mice
title_full_unstemmed	Potential of stem cell therapy during dengue infection in mice
title_sort	potential of stem cell therapy during dengue infection in mice
granting_institution	Universiti Putra Malaysia
publishDate	2017
url	http://psasir.upm.edu.my/id/eprint/70972/1/FPSK%28M%29%202017%2014%20-%20IR.pdf
_version_	1747812946408374272

Potential of stem cell therapy during dengue infection in mice

Similar Items