Evaluation of Vaca and Caga Genotypes of Helicobacter Pylori in Iranian Patients with Peptic Ulcer Disease
Helicobacter pylori infection occurs all over the world, and more than half of the world population is infected by this microorganism. Research on the variety of H. pylori genes is valuable from two perspectives; first, for predicting the outcome of the infection and second, for better understand...
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2007
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my-upm-ir.71612013-05-27T07:33:47Z Evaluation of Vaca and Caga Genotypes of Helicobacter Pylori in Iranian Patients with Peptic Ulcer Disease 2007 Hossein Abadi, Ali Saber Helicobacter pylori infection occurs all over the world, and more than half of the world population is infected by this microorganism. Research on the variety of H. pylori genes is valuable from two perspectives; first, for predicting the outcome of the infection and second, for better understanding of its distribution in the world and the evolutionary origins of this organism. It has been suggested that Helicobacter pylori strains containing cagA gene and the s1/m1 genotype of vacuolating cytotoxin gene A (vacA) may be associated with peptic ulcer diseases. Some studies have also shown that allele s1 of the vacA gene is associated with gastroduodenal diseases In order to investigate the cagA and vacA genes, biopsies of the antrum and corpus of the stomachs of patients were obtained. To detect H. pylori infection, the phosphoglucosamine mutase gene (glmM) was amplified through the PCR method and observed on 2% (w/v) agarose gel electrophoresis. All the H. pylori-positive samples were subjected to further PCR amplification to determine different alleles of the vacA gene. The PCR products were separated on 2% (w/v) agarose gels electrophoresis. 37, 15 and 32 out of 84 specimens were duodenal ulcer (DU), gastric ulcer (GU) and gastritis (GT), respectively. Seventy-seven (91.7%, χ2= 58.333, p < 0.05) out of 84 samples were H. pylori-positive. cagA gene was detected in 80% (χ2= 12.6, p < 0.001), 76.9% (χ2= 3.769, p > 0.05), and 48.3% (χ2= 0.034 p > 0.05) from DU, GU and GT samples, respectively. It was found that 66% (23/35) of DU samples, 62% (8/13) of GU samples and none of 29 GT samples were s1/m1. 17% (6/35) of DU samples, 15% (2/13) of GU samples and 52% (16/29) of GT samples were s1/m2. 17% (6/35) of DU samples, 23% (3/13) of GU samples and 48% (13/29) of GT samples were s2/m2. This study demonstrates that the presence of the m2 allele of vacA is strongly associated with gastritis and the presence of allele s1 is associated with peptic ulcers. Helicobacter pylori strains with vacA-s1/m2-cagA+ genotype are associated with peptic ulceration diseases. Peptic ulcer 2007 Thesis http://psasir.upm.edu.my/id/eprint/7161/ http://psasir.upm.edu.my/id/eprint/7161/1/FPSK%28M%29_2009_4a.pdf application/pdf en public masters Universiti Putra Malaysia Peptic ulcer Medicine and Health Sciences English |
| institution |
Universiti Putra Malaysia |
| collection |
PSAS Institutional Repository |
| language |
English English |
| topic |
Peptic ulcer |
| spellingShingle |
Peptic ulcer Hossein Abadi, Ali Saber Evaluation of Vaca and Caga Genotypes of Helicobacter Pylori in Iranian Patients with Peptic Ulcer Disease |
| description |
Helicobacter pylori infection occurs all over the world, and more than half of the world
population is infected by this microorganism. Research on the variety of H. pylori genes
is valuable from two perspectives; first, for predicting the outcome of the infection and
second, for better understanding of its distribution in the world and the evolutionary
origins of this organism.
It has been suggested that Helicobacter pylori strains containing cagA gene and the
s1/m1 genotype of vacuolating cytotoxin gene A (vacA) may be associated with peptic
ulcer diseases. Some studies have also shown that allele s1 of the vacA gene is
associated with gastroduodenal diseases In order to investigate the cagA and vacA genes, biopsies of the antrum and corpus of the
stomachs of patients were obtained. To detect H. pylori infection, the
phosphoglucosamine mutase gene (glmM) was amplified through the PCR method and
observed on 2% (w/v) agarose gel electrophoresis. All the H. pylori-positive samples
were subjected to further PCR amplification to determine different alleles of the vacA
gene. The PCR products were separated on 2% (w/v) agarose gels electrophoresis. 37,
15 and 32 out of 84 specimens were duodenal ulcer (DU), gastric ulcer (GU) and
gastritis (GT), respectively. Seventy-seven (91.7%, χ2= 58.333, p < 0.05) out of 84
samples were H. pylori-positive. cagA gene was detected in 80% (χ2= 12.6, p < 0.001),
76.9% (χ2= 3.769, p > 0.05), and 48.3% (χ2= 0.034 p > 0.05) from DU, GU and GT
samples, respectively. It was found that 66% (23/35) of DU samples, 62% (8/13) of GU
samples and none of 29 GT samples were s1/m1. 17% (6/35) of DU samples, 15%
(2/13) of GU samples and 52% (16/29) of GT samples were s1/m2. 17% (6/35) of DU
samples, 23% (3/13) of GU samples and 48% (13/29) of GT samples were s2/m2.
This study demonstrates that the presence of the m2 allele of vacA is strongly associated
with gastritis and the presence of allele s1 is associated with peptic ulcers. Helicobacter
pylori strains with vacA-s1/m2-cagA+ genotype are associated with peptic ulceration
diseases. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Hossein Abadi, Ali Saber |
| author_facet |
Hossein Abadi, Ali Saber |
| author_sort |
Hossein Abadi, Ali Saber |
| title |
Evaluation of Vaca and Caga Genotypes of Helicobacter Pylori in Iranian Patients with Peptic Ulcer Disease |
| title_short |
Evaluation of Vaca and Caga Genotypes of Helicobacter Pylori in Iranian Patients with Peptic Ulcer Disease |
| title_full |
Evaluation of Vaca and Caga Genotypes of Helicobacter Pylori in Iranian Patients with Peptic Ulcer Disease |
| title_fullStr |
Evaluation of Vaca and Caga Genotypes of Helicobacter Pylori in Iranian Patients with Peptic Ulcer Disease |
| title_full_unstemmed |
Evaluation of Vaca and Caga Genotypes of Helicobacter Pylori in Iranian Patients with Peptic Ulcer Disease |
| title_sort |
evaluation of vaca and caga genotypes of helicobacter pylori in iranian patients with peptic ulcer disease |
| granting_institution |
Universiti Putra Malaysia |
| granting_department |
Medicine and Health Sciences |
| publishDate |
2007 |
| url |
http://psasir.upm.edu.my/id/eprint/7161/1/FPSK%28M%29_2009_4a.pdf |
| _version_ |
1747810665601433600 |