Cytotoxic Effect of Betulinic Acid on Vascular Smooth Muscle Cells

Betulinic Acid (BA) is a widely available plant-derived triterpene reported as a selective cytotoxic activity against cancer cell of neuroectodermal origin and leukaemias. Interestingly, this will be first report to demonstrate the cytotoxic effect of BA in VSMCs. First, MTT cytotoxic assay was used...

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Main Author:	Vadivelu, Raja Kumar
Format:	Thesis
Language:	English English
Published:	2009
Subjects:	Acids Vascular smooth muscle
Online Access:	http://psasir.upm.edu.my/id/eprint/7220/1/IB_2009_6a.pdf
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spelling	my-upm-ir.72202013-05-27T07:34:10Z Cytotoxic Effect of Betulinic Acid on Vascular Smooth Muscle Cells 2009-04 Vadivelu, Raja Kumar Betulinic Acid (BA) is a widely available plant-derived triterpene reported as a selective cytotoxic activity against cancer cell of neuroectodermal origin and leukaemias. Interestingly, this will be first report to demonstrate the cytotoxic effect of BA in VSMCs. First, MTT cytotoxic assay was used to measure cell viability of VSMCs with predetermined concentrations of BA for 24h, 48h and 72h. The results obtain indicated that BA inhibit the growth and proliferation of VSMCs in a dose dependent manner IC10 of 0.4μg/ml, IC25 of 1μg/ml and IC50 of 3.8 μg/ml significantly (P<0.05). Secondly, the genotoxic potential associated to exposure to BA was assessed on VSMCs in vitro by the comet assay. BA exhibits low level of DNA damage and not likely to increase the level of DNA damage after 24 h exposure. Moreover, Flow cytometric analysis revealed that BA treatment FOR 24 h induced cell cycle arrest at the G1 phase and caused the appearance of a sub-G1 DNA peak at 48 h. Finally, the cell death morphology indicates that the percentage of apoptotic at 24 h were 12.43 ± 1.55% and at 48 h were 23.17 ± 1.73%, the percentage of necrotic cells at 48 h were 14.63 ± 1.45%. An increase percentage of apoptotic cells at 72 h were 45.92 ± 1.45%. In conclusion, exposure of BA to VSMCs initiate early DNA damage, arrest at G1 phase and induce apoptosis. Acids Vascular smooth muscle 2009-04 Thesis http://psasir.upm.edu.my/id/eprint/7220/ http://psasir.upm.edu.my/id/eprint/7220/1/IB_2009_6a.pdf application/pdf en public masters Universiti Putra Malaysia Acids Vascular smooth muscle Institute Bioscience English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Acids Vascular smooth muscle
spellingShingle	Acids Vascular smooth muscle Vadivelu, Raja Kumar Cytotoxic Effect of Betulinic Acid on Vascular Smooth Muscle Cells
description	Betulinic Acid (BA) is a widely available plant-derived triterpene reported as a selective cytotoxic activity against cancer cell of neuroectodermal origin and leukaemias. Interestingly, this will be first report to demonstrate the cytotoxic effect of BA in VSMCs. First, MTT cytotoxic assay was used to measure cell viability of VSMCs with predetermined concentrations of BA for 24h, 48h and 72h. The results obtain indicated that BA inhibit the growth and proliferation of VSMCs in a dose dependent manner IC10 of 0.4μg/ml, IC25 of 1μg/ml and IC50 of 3.8 μg/ml significantly (P<0.05). Secondly, the genotoxic potential associated to exposure to BA was assessed on VSMCs in vitro by the comet assay. BA exhibits low level of DNA damage and not likely to increase the level of DNA damage after 24 h exposure. Moreover, Flow cytometric analysis revealed that BA treatment FOR 24 h induced cell cycle arrest at the G1 phase and caused the appearance of a sub-G1 DNA peak at 48 h. Finally, the cell death morphology indicates that the percentage of apoptotic at 24 h were 12.43 ± 1.55% and at 48 h were 23.17 ± 1.73%, the percentage of necrotic cells at 48 h were 14.63 ± 1.45%. An increase percentage of apoptotic cells at 72 h were 45.92 ± 1.45%. In conclusion, exposure of BA to VSMCs initiate early DNA damage, arrest at G1 phase and induce apoptosis.
format	Thesis
qualification_level	Master's degree
author	Vadivelu, Raja Kumar
author_facet	Vadivelu, Raja Kumar
author_sort	Vadivelu, Raja Kumar
title	Cytotoxic Effect of Betulinic Acid on Vascular Smooth Muscle Cells
title_short	Cytotoxic Effect of Betulinic Acid on Vascular Smooth Muscle Cells
title_full	Cytotoxic Effect of Betulinic Acid on Vascular Smooth Muscle Cells
title_fullStr	Cytotoxic Effect of Betulinic Acid on Vascular Smooth Muscle Cells
title_full_unstemmed	Cytotoxic Effect of Betulinic Acid on Vascular Smooth Muscle Cells
title_sort	cytotoxic effect of betulinic acid on vascular smooth muscle cells
granting_institution	Universiti Putra Malaysia
granting_department	Institute Bioscience
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/7220/1/IB_2009_6a.pdf
_version_	1747810676629307392

Cytotoxic Effect of Betulinic Acid on Vascular Smooth Muscle Cells

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