Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia

Introduction: Carbapenems are the primary choice of treatment for nosocomial Pseudomonas aeruginosa infections. However, the emergence of carbapenem resistance due to the production of metallo-β-lactamases (MBLs) is a worldwide concern. Objective: To determine the prevalence of P. aeruginosa produci...

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Main Author:	Idris, Siti Nur Atiqah
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Pseudomonas aeruginosa - Chemistry Pseudomonas Infections - Therapy
Online Access:	http://psasir.upm.edu.my/id/eprint/75318/1/FPSK%28M%29%202016%2034%20IR.pdf
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spelling	my-upm-ir.753182019-10-17T08:44:10Z Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia 2016-05 Idris, Siti Nur Atiqah Introduction: Carbapenems are the primary choice of treatment for nosocomial Pseudomonas aeruginosa infections. However, the emergence of carbapenem resistance due to the production of metallo-β-lactamases (MBLs) is a worldwide concern. Objective: To determine the prevalence of P. aeruginosa producing metallo-β-lactamase isolated from clinical isolates at Hospital Kuala Lumpur (HKL), Malaysia. Method: A total of 83 P. aeruginosa isolates were collected and tested for metallo-β-lactamase production using a phenotypic combined disc method and genotypic detection by polymerase chain reaction (PCR) and random amplified polymorphic DNA (RAPD). Results: Of all the isolates, 52% were found to be imipenem resistant P. aeruginosa and from that, 65% of the isolates showed positive result by phenotypic imipenem-EDTA combined disc test in which there were an increase in inhibition zone on imipenem with EDTA as compared to imipenem alone. MBL genes were detected from polymerase chain reaction (PCR) in 35% of total isolates (strains producing blaIMP were 45% followed by blaVIM, blaSPM and blaGIM with 34%, 14% and 7%, respectively. RAPD analysis showed a total of 8 clusters among the 83 P. aeruginosa clinical isolates. Conclusion: Our study highlight that blaIMP and blaVIM were predominantly present among the P. aeruginosa clinical isolates from Hospital Kuala Lumpur. Keywords: Pseudomonas aeruginosa, imipenem resistance, metallo-β-lactamases (MBLs), blaIMP, blaVIM, blaSPM and blaGIM. Pseudomonas aeruginosa - Chemistry Pseudomonas Infections - Therapy 2016-05 Thesis http://psasir.upm.edu.my/id/eprint/75318/ http://psasir.upm.edu.my/id/eprint/75318/1/FPSK%28M%29%202016%2034%20IR.pdf text en public masters Universiti Putra Malaysia Pseudomonas aeruginosa - Chemistry Pseudomonas Infections - Therapy
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Pseudomonas aeruginosa - Chemistry Pseudomonas Infections - Therapy
spellingShingle	Pseudomonas aeruginosa - Chemistry Pseudomonas Infections - Therapy Idris, Siti Nur Atiqah Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia
description	Introduction: Carbapenems are the primary choice of treatment for nosocomial Pseudomonas aeruginosa infections. However, the emergence of carbapenem resistance due to the production of metallo-β-lactamases (MBLs) is a worldwide concern. Objective: To determine the prevalence of P. aeruginosa producing metallo-β-lactamase isolated from clinical isolates at Hospital Kuala Lumpur (HKL), Malaysia. Method: A total of 83 P. aeruginosa isolates were collected and tested for metallo-β-lactamase production using a phenotypic combined disc method and genotypic detection by polymerase chain reaction (PCR) and random amplified polymorphic DNA (RAPD). Results: Of all the isolates, 52% were found to be imipenem resistant P. aeruginosa and from that, 65% of the isolates showed positive result by phenotypic imipenem-EDTA combined disc test in which there were an increase in inhibition zone on imipenem with EDTA as compared to imipenem alone. MBL genes were detected from polymerase chain reaction (PCR) in 35% of total isolates (strains producing blaIMP were 45% followed by blaVIM, blaSPM and blaGIM with 34%, 14% and 7%, respectively. RAPD analysis showed a total of 8 clusters among the 83 P. aeruginosa clinical isolates. Conclusion: Our study highlight that blaIMP and blaVIM were predominantly present among the P. aeruginosa clinical isolates from Hospital Kuala Lumpur. Keywords: Pseudomonas aeruginosa, imipenem resistance, metallo-β-lactamases (MBLs), blaIMP, blaVIM, blaSPM and blaGIM.
format	Thesis
qualification_level	Master's degree
author	Idris, Siti Nur Atiqah
author_facet	Idris, Siti Nur Atiqah
author_sort	Idris, Siti Nur Atiqah
title	Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia
title_short	Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia
title_full	Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia
title_fullStr	Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia
title_full_unstemmed	Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia
title_sort	prevalence of metallo-beta-lactamase producing pseudomonas aeruginosa in a tertiary care at hospital kuala lumpur, malaysia
granting_institution	Universiti Putra Malaysia
publishDate	2016
url	http://psasir.upm.edu.my/id/eprint/75318/1/FPSK%28M%29%202016%2034%20IR.pdf
_version_	1747813030515703808

Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia

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