Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia
Introduction: Carbapenems are the primary choice of treatment for nosocomial Pseudomonas aeruginosa infections. However, the emergence of carbapenem resistance due to the production of metallo-β-lactamases (MBLs) is a worldwide concern. Objective: To determine the prevalence of P. aeruginosa produci...
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my-upm-ir.753182019-10-17T08:44:10Z Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia 2016-05 Idris, Siti Nur Atiqah Introduction: Carbapenems are the primary choice of treatment for nosocomial Pseudomonas aeruginosa infections. However, the emergence of carbapenem resistance due to the production of metallo-β-lactamases (MBLs) is a worldwide concern. Objective: To determine the prevalence of P. aeruginosa producing metallo-β-lactamase isolated from clinical isolates at Hospital Kuala Lumpur (HKL), Malaysia. Method: A total of 83 P. aeruginosa isolates were collected and tested for metallo-β-lactamase production using a phenotypic combined disc method and genotypic detection by polymerase chain reaction (PCR) and random amplified polymorphic DNA (RAPD). Results: Of all the isolates, 52% were found to be imipenem resistant P. aeruginosa and from that, 65% of the isolates showed positive result by phenotypic imipenem-EDTA combined disc test in which there were an increase in inhibition zone on imipenem with EDTA as compared to imipenem alone. MBL genes were detected from polymerase chain reaction (PCR) in 35% of total isolates (strains producing blaIMP were 45% followed by blaVIM, blaSPM and blaGIM with 34%, 14% and 7%, respectively. RAPD analysis showed a total of 8 clusters among the 83 P. aeruginosa clinical isolates. Conclusion: Our study highlight that blaIMP and blaVIM were predominantly present among the P. aeruginosa clinical isolates from Hospital Kuala Lumpur. Keywords: Pseudomonas aeruginosa, imipenem resistance, metallo-β-lactamases (MBLs), blaIMP, blaVIM, blaSPM and blaGIM. Pseudomonas aeruginosa - Chemistry Pseudomonas Infections - Therapy 2016-05 Thesis http://psasir.upm.edu.my/id/eprint/75318/ http://psasir.upm.edu.my/id/eprint/75318/1/FPSK%28M%29%202016%2034%20IR.pdf text en public masters Universiti Putra Malaysia Pseudomonas aeruginosa - Chemistry Pseudomonas Infections - Therapy |
| institution |
Universiti Putra Malaysia |
| collection |
PSAS Institutional Repository |
| language |
English |
| topic |
Pseudomonas aeruginosa - Chemistry Pseudomonas Infections - Therapy |
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Pseudomonas aeruginosa - Chemistry Pseudomonas Infections - Therapy Idris, Siti Nur Atiqah Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia |
| description |
Introduction: Carbapenems are the primary choice of treatment for nosocomial Pseudomonas aeruginosa infections. However, the emergence of carbapenem resistance due to the production of metallo-β-lactamases (MBLs) is a worldwide concern. Objective: To determine the prevalence of P. aeruginosa producing metallo-β-lactamase isolated from clinical isolates at Hospital Kuala Lumpur (HKL), Malaysia. Method: A total of 83 P. aeruginosa isolates were collected and tested for metallo-β-lactamase production using a phenotypic combined disc method and genotypic detection by polymerase chain reaction (PCR) and random amplified polymorphic DNA (RAPD). Results: Of all the isolates, 52% were found to be imipenem resistant P. aeruginosa and from that, 65% of the isolates showed positive result by phenotypic imipenem-EDTA combined disc test in which there were an increase in inhibition zone on imipenem with EDTA as compared to imipenem alone. MBL genes were detected from polymerase chain reaction (PCR) in 35% of total isolates (strains producing blaIMP were 45% followed by blaVIM, blaSPM and blaGIM with 34%, 14% and 7%, respectively. RAPD analysis showed a total of 8 clusters among the 83 P. aeruginosa clinical isolates. Conclusion: Our study highlight that blaIMP and blaVIM were predominantly present among the P. aeruginosa clinical isolates from Hospital Kuala Lumpur. Keywords: Pseudomonas aeruginosa, imipenem resistance, metallo-β-lactamases (MBLs), blaIMP, blaVIM, blaSPM and blaGIM. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Idris, Siti Nur Atiqah |
| author_facet |
Idris, Siti Nur Atiqah |
| author_sort |
Idris, Siti Nur Atiqah |
| title |
Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia |
| title_short |
Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia |
| title_full |
Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia |
| title_fullStr |
Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia |
| title_full_unstemmed |
Prevalence of metallo-Beta-lactamase producing Pseudomonas aeruginosa in a tertiary care at Hospital Kuala Lumpur, Malaysia |
| title_sort |
prevalence of metallo-beta-lactamase producing pseudomonas aeruginosa in a tertiary care at hospital kuala lumpur, malaysia |
| granting_institution |
Universiti Putra Malaysia |
| publishDate |
2016 |
| url |
http://psasir.upm.edu.my/id/eprint/75318/1/FPSK%28M%29%202016%2034%20IR.pdf |
| _version_ |
1747813030515703808 |