Hedgehog pathway proteins and their association with clinicopathological parameters of bladder cancer

Hedgehog pathway is important for growth and patterning during embryonic development. Previous studies have shown that constitutive activation of Hedgehog pathway can lead to various types of malignancies including medulloblastoma, basal cell carcinoma, gastrointestinal, breast and prostate cancer....

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主要作者:	Mohd Ariffin, Khairunnisa
格式:	Thesis
語言:	English
出版:	2016
主題:	Hedgehog Proteins Urinary Bladder Neoplasms
在線閱讀:	http://psasir.upm.edu.my/id/eprint/75339/1/FPSK%28M%29%202016%2042%20IR.pdf
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id	my-upm-ir.75339
record_format	uketd_dc
spelling	my-upm-ir.753392019-11-25T08:01:31Z Hedgehog pathway proteins and their association with clinicopathological parameters of bladder cancer 2016-08 Mohd Ariffin, Khairunnisa Hedgehog pathway is important for growth and patterning during embryonic development. Previous studies have shown that constitutive activation of Hedgehog pathway can lead to various types of malignancies including medulloblastoma, basal cell carcinoma, gastrointestinal, breast and prostate cancer. The purpose of this study was to investigate the expression of Hedgehog pathway proteins in bladder cancer and determine their association with clinicopathological parameters. The expression of Sonic hedgehog (SHH), its receptor Patched (PTCH1), downstream transcription factor GLI1 and its signal transducer SMO in 112 bladder cancer tissues from Hospital Kuala Lumpur were determined by immunohistochemistry. SHH was expressed in 108 (96.4%) cases, GLI1 in 104 (92.9%) cases, PTCH1 in 111 (99.1%) cases and SMO in all cases. The relationship between the expression of these four proteins and its clinicopathological parameters were analysed statistically. Immunohistochemical staining results showed SHH, GLI1 and SMO proteins were mainly located in the cytoplasm of tumour cells, whereas PTCH1 was mainly located in the nucleus of tumour cells. Positive expression of SHH, PTCH1, GLI1 and SMO proteins were correlated with a few variables which include grade and stage of bladder cancer, lymph node metastasis and distant metastasis (Mx). Among all of the clinicopathological parameters, SMO was found to be the only protein that showed statistically significant association with higher grade (p=0.001) and higher stage (p=0.042) of bladder cancer. SMO expression also showed borderline association with lymph node metastasis (p=0.056). These findings indicate that SMO expression may be a poor prognostic marker in bladder cancer. Hedgehog Proteins Urinary Bladder Neoplasms 2016-08 Thesis http://psasir.upm.edu.my/id/eprint/75339/ http://psasir.upm.edu.my/id/eprint/75339/1/FPSK%28M%29%202016%2042%20IR.pdf text en public masters Universiti Putra Malaysia Hedgehog Proteins Urinary Bladder Neoplasms
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Hedgehog Proteins Urinary Bladder Neoplasms
spellingShingle	Hedgehog Proteins Urinary Bladder Neoplasms Mohd Ariffin, Khairunnisa Hedgehog pathway proteins and their association with clinicopathological parameters of bladder cancer
description	Hedgehog pathway is important for growth and patterning during embryonic development. Previous studies have shown that constitutive activation of Hedgehog pathway can lead to various types of malignancies including medulloblastoma, basal cell carcinoma, gastrointestinal, breast and prostate cancer. The purpose of this study was to investigate the expression of Hedgehog pathway proteins in bladder cancer and determine their association with clinicopathological parameters. The expression of Sonic hedgehog (SHH), its receptor Patched (PTCH1), downstream transcription factor GLI1 and its signal transducer SMO in 112 bladder cancer tissues from Hospital Kuala Lumpur were determined by immunohistochemistry. SHH was expressed in 108 (96.4%) cases, GLI1 in 104 (92.9%) cases, PTCH1 in 111 (99.1%) cases and SMO in all cases. The relationship between the expression of these four proteins and its clinicopathological parameters were analysed statistically. Immunohistochemical staining results showed SHH, GLI1 and SMO proteins were mainly located in the cytoplasm of tumour cells, whereas PTCH1 was mainly located in the nucleus of tumour cells. Positive expression of SHH, PTCH1, GLI1 and SMO proteins were correlated with a few variables which include grade and stage of bladder cancer, lymph node metastasis and distant metastasis (Mx). Among all of the clinicopathological parameters, SMO was found to be the only protein that showed statistically significant association with higher grade (p=0.001) and higher stage (p=0.042) of bladder cancer. SMO expression also showed borderline association with lymph node metastasis (p=0.056). These findings indicate that SMO expression may be a poor prognostic marker in bladder cancer.
format	Thesis
qualification_level	Master's degree
author	Mohd Ariffin, Khairunnisa
author_facet	Mohd Ariffin, Khairunnisa
author_sort	Mohd Ariffin, Khairunnisa
title	Hedgehog pathway proteins and their association with clinicopathological parameters of bladder cancer
title_short	Hedgehog pathway proteins and their association with clinicopathological parameters of bladder cancer
title_full	Hedgehog pathway proteins and their association with clinicopathological parameters of bladder cancer
title_fullStr	Hedgehog pathway proteins and their association with clinicopathological parameters of bladder cancer
title_full_unstemmed	Hedgehog pathway proteins and their association with clinicopathological parameters of bladder cancer
title_sort	hedgehog pathway proteins and their association with clinicopathological parameters of bladder cancer
granting_institution	Universiti Putra Malaysia
publishDate	2016
url	http://psasir.upm.edu.my/id/eprint/75339/1/FPSK%28M%29%202016%2042%20IR.pdf
_version_	1747813034677501952

Hedgehog pathway proteins and their association with clinicopathological parameters of bladder cancer

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