Chemical Constituents from Calophyllum Inophyllum and Cratoxylum Arborescens and Their Biological Activities

Chemical and biological studies were carried out on two plants, Calophyllum inophyllum (Guttiferae) and Cratoxylum arborescens (Guttiferae). The chemical investigation covered triterpenoids, quinones and xanthones. These compounds were isolated using common chromatographic techniques and were identi...

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Main Author:	Jong, Vivien Yi Mian
Format:	Thesis
Language:	English English
Published:	2007
Subjects:	Calophyllum inophyllum - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/7544/1/ABS_--__FS_2007_65.pdf
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spelling	my-upm-ir.75442013-05-27T07:35:30Z Chemical Constituents from Calophyllum Inophyllum and Cratoxylum Arborescens and Their Biological Activities 2007 Jong, Vivien Yi Mian Chemical and biological studies were carried out on two plants, Calophyllum inophyllum (Guttiferae) and Cratoxylum arborescens (Guttiferae). The chemical investigation covered triterpenoids, quinones and xanthones. These compounds were isolated using common chromatographic techniques and were identified using spectroscopic experiments such as NMR, MS, IR and UV. Calophyllum inophyllum (root bark) afforded sitosterol (71) and six other known xanthones which are brasilixanthone (72), inophyllin A (26), inophyllin B (27), 1,3,5-trihydroxy-2-methoxy xanthone (73), caloxanthone A (74) and pyranojacareubin (75). Meanwhile, studies on Cratoxylum arborescens (stem bark) provided one triterpenoid, friedelin (76) together with three others known quinones. These are vismione (77), vismiaquinone (78), and 1,8-dihydroxy-3-methoxy-6-methyl anthraquinone (79).Cytotoxic tests were carried out using CEM-SS cell line and HL-60 cell line. The crude hexane extract of Cratoxylum arborescens B. was found to be inactive to cytotoxic activity. The crude chloroform and methanol extracts of Cratoxylum arborescens B. showed good cytotoxic activity with IC50 values of 16 and 18 μg/ml, respectively. Meanwhile, the crude chloroform extract of the stem bark of Calophyllum inophyllum L. also showed a good cytotoxic activity with an IC50 value of 17 μg/ml. Two pure compounds obtained from Calophyllum inophyllum L., inophylin B (26) and caloxanthone A (74) also showed good activities against the HL-60 cell line with IC50 values of 15 and 29 μg/ml respectively. The antimicrobial activity test was also carried out using four pathogenic bacteria, namely, Methicilin Resistant Staphylococcus aures, Pseudomonas aeruginosa, Staphylococcus typhimurium and Bacillus subtilis. However, most of the crude extracts gave only moderate or weak activity. The larvicidal tests were performed against the larvae of Aedes aegypti using the WHO (1981) standard procedures with slight modifications. The crude hexane, chloroform and methanol extracts of Calophyllum inophyllum and Cratoxylum arborescens were inactive against the larvae of Aedes aegypti. No activity was recorded for the antifungal activity test Calophyllum inophyllum - Case studies 2007 Thesis http://psasir.upm.edu.my/id/eprint/7544/ http://psasir.upm.edu.my/id/eprint/7544/1/ABS_--__FS_2007_65.pdf application/pdf en public masters Universiti Putra Malaysia Calophyllum inophyllum - Case studies Faculty: Science English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Calophyllum inophyllum - Case studies
spellingShingle	Calophyllum inophyllum - Case studies Jong, Vivien Yi Mian Chemical Constituents from Calophyllum Inophyllum and Cratoxylum Arborescens and Their Biological Activities
description	Chemical and biological studies were carried out on two plants, Calophyllum inophyllum (Guttiferae) and Cratoxylum arborescens (Guttiferae). The chemical investigation covered triterpenoids, quinones and xanthones. These compounds were isolated using common chromatographic techniques and were identified using spectroscopic experiments such as NMR, MS, IR and UV. Calophyllum inophyllum (root bark) afforded sitosterol (71) and six other known xanthones which are brasilixanthone (72), inophyllin A (26), inophyllin B (27), 1,3,5-trihydroxy-2-methoxy xanthone (73), caloxanthone A (74) and pyranojacareubin (75). Meanwhile, studies on Cratoxylum arborescens (stem bark) provided one triterpenoid, friedelin (76) together with three others known quinones. These are vismione (77), vismiaquinone (78), and 1,8-dihydroxy-3-methoxy-6-methyl anthraquinone (79).Cytotoxic tests were carried out using CEM-SS cell line and HL-60 cell line. The crude hexane extract of Cratoxylum arborescens B. was found to be inactive to cytotoxic activity. The crude chloroform and methanol extracts of Cratoxylum arborescens B. showed good cytotoxic activity with IC50 values of 16 and 18 μg/ml, respectively. Meanwhile, the crude chloroform extract of the stem bark of Calophyllum inophyllum L. also showed a good cytotoxic activity with an IC50 value of 17 μg/ml. Two pure compounds obtained from Calophyllum inophyllum L., inophylin B (26) and caloxanthone A (74) also showed good activities against the HL-60 cell line with IC50 values of 15 and 29 μg/ml respectively. The antimicrobial activity test was also carried out using four pathogenic bacteria, namely, Methicilin Resistant Staphylococcus aures, Pseudomonas aeruginosa, Staphylococcus typhimurium and Bacillus subtilis. However, most of the crude extracts gave only moderate or weak activity. The larvicidal tests were performed against the larvae of Aedes aegypti using the WHO (1981) standard procedures with slight modifications. The crude hexane, chloroform and methanol extracts of Calophyllum inophyllum and Cratoxylum arborescens were inactive against the larvae of Aedes aegypti. No activity was recorded for the antifungal activity test
format	Thesis
qualification_level	Master's degree
author	Jong, Vivien Yi Mian
author_facet	Jong, Vivien Yi Mian
author_sort	Jong, Vivien Yi Mian
title	Chemical Constituents from Calophyllum Inophyllum and Cratoxylum Arborescens and Their Biological Activities
title_short	Chemical Constituents from Calophyllum Inophyllum and Cratoxylum Arborescens and Their Biological Activities
title_full	Chemical Constituents from Calophyllum Inophyllum and Cratoxylum Arborescens and Their Biological Activities
title_fullStr	Chemical Constituents from Calophyllum Inophyllum and Cratoxylum Arborescens and Their Biological Activities
title_full_unstemmed	Chemical Constituents from Calophyllum Inophyllum and Cratoxylum Arborescens and Their Biological Activities
title_sort	chemical constituents from calophyllum inophyllum and cratoxylum arborescens and their biological activities
granting_institution	Universiti Putra Malaysia
granting_department	Faculty: Science
publishDate	2007
url	http://psasir.upm.edu.my/id/eprint/7544/1/ABS_--__FS_2007_65.pdf
_version_	1747810697652207616

Chemical Constituents from Calophyllum Inophyllum and Cratoxylum Arborescens and Their Biological Activities

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