Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent

Biological hydrogen production offers a method through which biomass can be utilized for production of premium-energy carrier as an alternative energy for replacement of non-renewable fossil fuels. The present work aims to elucidate the substrate utilization in biohydrogen production from palm oil m...

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Main Author:	Taifor, Azam Fikri
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Hydrogen - Biotechnology Biochemical engineering Oil palm
Online Access:	http://psasir.upm.edu.my/id/eprint/75591/1/FBSB%202018%2021%20-%20IR.pdf
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id	my-upm-ir.75591
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spelling	my-upm-ir.755912019-11-27T01:33:17Z Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent 2017-11 Taifor, Azam Fikri Biological hydrogen production offers a method through which biomass can be utilized for production of premium-energy carrier as an alternative energy for replacement of non-renewable fossil fuels. The present work aims to elucidate the substrate utilization in biohydrogen production from palm oil mill effluent (POME) by E. coli strains. The experiments were performed in 150 mL serum bottles and the cultures were supplemented with autoclaved-pretreated POME to investigate the potential use of various carbon sources and its preference towards biohydrogen production. The cultures conditions were maintained at 37 C for 24 h with mild agitation at 120 rpm. The maximum hydrogen yield (MHY) of 0.68 mol H2 / mol total sugars with 81% substrate consumption based on total sugar and hydrogen productivity of 3552 mol / 1010 cfu were obtained from engineered E. coli BW25113 after 24 h of fermentation. The biohydrogen production by modified strain was enhanced by 3.5 fold compared to wild type strain. On the other hand, studies on the utilization of various substrates on the synthetic medium showed that the highest MHY of 0.77 mol H2 / mol total sugar was obtained from fermentation of fructose by engineered E. coli BW25113. The preference of the substrates based on the synthetic medium for biohydrogen production was in the following order; fructose > glucose > formic acid. These findings indicated that the use of modified strain E. coli BW25113 has enhanced biohydrogen production from POME. Hydrogen - Biotechnology Biochemical engineering Oil palm 2017-11 Thesis http://psasir.upm.edu.my/id/eprint/75591/ http://psasir.upm.edu.my/id/eprint/75591/1/FBSB%202018%2021%20-%20IR.pdf text en public masters Universiti Putra Malaysia Hydrogen - Biotechnology Biochemical engineering Oil palm
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Hydrogen - Biotechnology Biochemical engineering Oil palm
spellingShingle	Hydrogen - Biotechnology Biochemical engineering Oil palm Taifor, Azam Fikri Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
description	Biological hydrogen production offers a method through which biomass can be utilized for production of premium-energy carrier as an alternative energy for replacement of non-renewable fossil fuels. The present work aims to elucidate the substrate utilization in biohydrogen production from palm oil mill effluent (POME) by E. coli strains. The experiments were performed in 150 mL serum bottles and the cultures were supplemented with autoclaved-pretreated POME to investigate the potential use of various carbon sources and its preference towards biohydrogen production. The cultures conditions were maintained at 37 C for 24 h with mild agitation at 120 rpm. The maximum hydrogen yield (MHY) of 0.68 mol H2 / mol total sugars with 81% substrate consumption based on total sugar and hydrogen productivity of 3552 mol / 1010 cfu were obtained from engineered E. coli BW25113 after 24 h of fermentation. The biohydrogen production by modified strain was enhanced by 3.5 fold compared to wild type strain. On the other hand, studies on the utilization of various substrates on the synthetic medium showed that the highest MHY of 0.77 mol H2 / mol total sugar was obtained from fermentation of fructose by engineered E. coli BW25113. The preference of the substrates based on the synthetic medium for biohydrogen production was in the following order; fructose > glucose > formic acid. These findings indicated that the use of modified strain E. coli BW25113 has enhanced biohydrogen production from POME.
format	Thesis
qualification_level	Master's degree
author	Taifor, Azam Fikri
author_facet	Taifor, Azam Fikri
author_sort	Taifor, Azam Fikri
title	Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
title_short	Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
title_full	Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
title_fullStr	Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
title_full_unstemmed	Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
title_sort	use of metabolic engineered escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
granting_institution	Universiti Putra Malaysia
publishDate	2017
url	http://psasir.upm.edu.my/id/eprint/75591/1/FBSB%202018%2021%20-%20IR.pdf
_version_	1747813068330500096

Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent

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