Biological Activities of Barringtonia Racemosa (Lecythidaceae) and the Quantitative Analysis of Phenolic Acids And Flavonoids Present in the Various Bioactive Extracts of the Species

Barringtonia racemosa (Family Lecythidaceae) posses several bioactivities and is used in traditional medicine of Malaysia. However, its antioxidant, antimicrobial, anti-inflammatory and allelopathic activities investigation are still lacking. The aims of this study were therefore to determine those...

Full description

Saved in:

Bibliographic Details
Main Author:	Hussin, Nurul Mariam
Format:	Thesis
Language:	English English
Published:	2009
Subjects:	Lecythidaceae - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/7577/1/ABS_----_FBSB_2009_24.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.7577
record_format	uketd_dc
spelling	my-upm-ir.75772013-05-27T07:35:43Z Biological Activities of Barringtonia Racemosa (Lecythidaceae) and the Quantitative Analysis of Phenolic Acids And Flavonoids Present in the Various Bioactive Extracts of the Species 2009 Hussin, Nurul Mariam Barringtonia racemosa (Family Lecythidaceae) posses several bioactivities and is used in traditional medicine of Malaysia. However, its antioxidant, antimicrobial, anti-inflammatory and allelopathic activities investigation are still lacking. The aims of this study were therefore to determine those biological activities of methanolic, ethanolic and hot water leaf, stem and bark extracts of B. racemosa and quantitative analysis of phenolic acids and flavonoids present in the various bioactive extracts of the species. The methanolic and ethanolic extracts of bark showed strong free radical scavenging activity (89.23 ± 0.40 and 91.35 ± 0.89 %) and this was even higher than butylated hydroxytoluene (BHT), ascorbic acid and α-tocopherol (antioxidant standards). The results also showed that all methanolic extracts (leaf, stem, and bark; 84.47 ± 0.05, 84.41 ± 0.28 and 84.03 ± 0.03 %) and ethanolic extracts of leaf and bark (82.58 ± 0.06 and 88.21 ± 0.06 %), respectively had similarly strong reducing power when compared to the antioxidant standards, tested in this study. The leaf of methanolic and ethanolic extracts showed strong antioxidant activity (80.03 ± 5.56 and 88.93 ± 8.32 %) in the β-carotene bleaching assay which were higher than the antioxidant standards. Although most of the hot water extracts showed weak to moderately antioxidant activities, the hot water extract of the leaf surprisingly showed strong reducing power potential (83.45 ± 0.07 %). Overall, the methanolic and ethanolic extracts of B. racemosa had effective antioxidant activities compared to hot water extracts. In Griess assay, all the B. racemosa extracts showed no significant activity on nitric oxide inhibition in RAW264.7 cell line. However, the extracts do not seem to be cytotoxic. In antibacterial assay, B. racemosa aerial parts extracts showed weak to moderate inhibitory activities against both Gram-positive and Gram-negative bacteria tested accept for boiling water extracts which was inactive. For antifungal assay, methanolic extracts of B. racemosa leaf, stem and bark exhibited the higher inhibitory activity among the four of seven fungi tested. Among different fungi tested, all extracts were found to be more sensitive to Fusarium sp. compared to the others. The seed germination assay results showed that B. racemosa extracts were able to effect the germination of the Brassica nigra L. (allelopathic activity). In the present study, quantitative analysis of phenolic compounds from B. racemosa leaf, stem and bark were performed using reversed-phase high performance liquid chromatography (RP-HPLC) technique. The results showed that gallic acid and naringin were the major phenolic acids and flavonoids present in all different aerial parts of B. racemosa. Ferulic acid, rutin, kaempferol and luteolin were also detected in several aerial parts of B. racemosa. Overall results obtained from the biological assays suggested that B. racemosa is a source of bioactive compounds endowed with interesting biological activities, such as strongly antioxidants agents and can be rated as good dietary sources of natural phenolic antioxidants. Lecythidaceae - Case studies 2009 Thesis http://psasir.upm.edu.my/id/eprint/7577/ http://psasir.upm.edu.my/id/eprint/7577/1/ABS_----_FBSB_2009_24.pdf application/pdf en public masters Universiti Putra Malaysia Lecythidaceae - Case studies Faculty of Biotechnology and Biomolecular Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Lecythidaceae - Case studies
spellingShingle	Lecythidaceae - Case studies Hussin, Nurul Mariam Biological Activities of Barringtonia Racemosa (Lecythidaceae) and the Quantitative Analysis of Phenolic Acids And Flavonoids Present in the Various Bioactive Extracts of the Species
description	Barringtonia racemosa (Family Lecythidaceae) posses several bioactivities and is used in traditional medicine of Malaysia. However, its antioxidant, antimicrobial, anti-inflammatory and allelopathic activities investigation are still lacking. The aims of this study were therefore to determine those biological activities of methanolic, ethanolic and hot water leaf, stem and bark extracts of B. racemosa and quantitative analysis of phenolic acids and flavonoids present in the various bioactive extracts of the species. The methanolic and ethanolic extracts of bark showed strong free radical scavenging activity (89.23 ± 0.40 and 91.35 ± 0.89 %) and this was even higher than butylated hydroxytoluene (BHT), ascorbic acid and α-tocopherol (antioxidant standards). The results also showed that all methanolic extracts (leaf, stem, and bark; 84.47 ± 0.05, 84.41 ± 0.28 and 84.03 ± 0.03 %) and ethanolic extracts of leaf and bark (82.58 ± 0.06 and 88.21 ± 0.06 %), respectively had similarly strong reducing power when compared to the antioxidant standards, tested in this study. The leaf of methanolic and ethanolic extracts showed strong antioxidant activity (80.03 ± 5.56 and 88.93 ± 8.32 %) in the β-carotene bleaching assay which were higher than the antioxidant standards. Although most of the hot water extracts showed weak to moderately antioxidant activities, the hot water extract of the leaf surprisingly showed strong reducing power potential (83.45 ± 0.07 %). Overall, the methanolic and ethanolic extracts of B. racemosa had effective antioxidant activities compared to hot water extracts. In Griess assay, all the B. racemosa extracts showed no significant activity on nitric oxide inhibition in RAW264.7 cell line. However, the extracts do not seem to be cytotoxic. In antibacterial assay, B. racemosa aerial parts extracts showed weak to moderate inhibitory activities against both Gram-positive and Gram-negative bacteria tested accept for boiling water extracts which was inactive. For antifungal assay, methanolic extracts of B. racemosa leaf, stem and bark exhibited the higher inhibitory activity among the four of seven fungi tested. Among different fungi tested, all extracts were found to be more sensitive to Fusarium sp. compared to the others. The seed germination assay results showed that B. racemosa extracts were able to effect the germination of the Brassica nigra L. (allelopathic activity). In the present study, quantitative analysis of phenolic compounds from B. racemosa leaf, stem and bark were performed using reversed-phase high performance liquid chromatography (RP-HPLC) technique. The results showed that gallic acid and naringin were the major phenolic acids and flavonoids present in all different aerial parts of B. racemosa. Ferulic acid, rutin, kaempferol and luteolin were also detected in several aerial parts of B. racemosa. Overall results obtained from the biological assays suggested that B. racemosa is a source of bioactive compounds endowed with interesting biological activities, such as strongly antioxidants agents and can be rated as good dietary sources of natural phenolic antioxidants.
format	Thesis
qualification_level	Master's degree
author	Hussin, Nurul Mariam
author_facet	Hussin, Nurul Mariam
author_sort	Hussin, Nurul Mariam
title	Biological Activities of Barringtonia Racemosa (Lecythidaceae) and the Quantitative Analysis of Phenolic Acids And Flavonoids Present in the Various Bioactive Extracts of the Species
title_short	Biological Activities of Barringtonia Racemosa (Lecythidaceae) and the Quantitative Analysis of Phenolic Acids And Flavonoids Present in the Various Bioactive Extracts of the Species
title_full	Biological Activities of Barringtonia Racemosa (Lecythidaceae) and the Quantitative Analysis of Phenolic Acids And Flavonoids Present in the Various Bioactive Extracts of the Species
title_fullStr	Biological Activities of Barringtonia Racemosa (Lecythidaceae) and the Quantitative Analysis of Phenolic Acids And Flavonoids Present in the Various Bioactive Extracts of the Species
title_full_unstemmed	Biological Activities of Barringtonia Racemosa (Lecythidaceae) and the Quantitative Analysis of Phenolic Acids And Flavonoids Present in the Various Bioactive Extracts of the Species
title_sort	biological activities of barringtonia racemosa (lecythidaceae) and the quantitative analysis of phenolic acids and flavonoids present in the various bioactive extracts of the species
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular Sciences
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/7577/1/ABS_----_FBSB_2009_24.pdf
_version_	1747810704064249856

Biological Activities of Barringtonia Racemosa (Lecythidaceae) and the Quantitative Analysis of Phenolic Acids And Flavonoids Present in the Various Bioactive Extracts of the Species

Similar Items