Production of cellulolytic enzymes via solid state fermentation of spent mushroom substrate by Trichoderma asperellum UPM 1

Spent mushroom substrate (SMS) is a by-product generated after mushroom harvesting from the commercial mushroom cultivation industry. SMS had lost a significant fraction of their mass (approximately 35-50% depending on their nature) due to metabolic reactions, mushroom production and water evaporati...

Full description

Saved in:

Bibliographic Details
Main Author:	Mohd Ariff, Iffah Nabilah
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Mushrooms Mushroom culture
Online Access:	http://psasir.upm.edu.my/id/eprint/75829/1/FBSB%202016%2044%20-%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.75829
record_format	uketd_dc
spelling	my-upm-ir.758292019-11-20T07:10:48Z Production of cellulolytic enzymes via solid state fermentation of spent mushroom substrate by Trichoderma asperellum UPM 1 2016-08 Mohd Ariff, Iffah Nabilah Spent mushroom substrate (SMS) is a by-product generated after mushroom harvesting from the commercial mushroom cultivation industry. SMS had lost a significant fraction of their mass (approximately 35-50% depending on their nature) due to metabolic reactions, mushroom production and water evaporation. SMS generally composed of three main components i.e. fungal mycelia, extracellular enzymes secreted during growing of mushroom and unutilized lignocellulosic components. Delignified SMS with small particle size (<1 mm) has been observed to have high cellulose component and lignocellulolytic enzyme content. SMS comprised of high cellulose (45.5%) and partially degraded lignin by the action of Pleurotus pulmonarius during mushroom cultivation. SMS in readily available smaller particle size offers a great advantage as a fermentation feedstock. Hence, owing to this untapped potential, this study has investigated the feasibility of SMS as an alternative substrate for lignocellulolytic enzyme recovery. This was done by profiling ligninolytic enzymes activities produced during the growth of P. pulmonarius and P. floridanus. Crude laccase (3 U/g and 2.6 U/g) and MnP (1.4 U/g and 2.1 U/g) were detected in SMS from P. pulmonarius and P. floridanus, respectively. After enzyme recovery, optimization of cellulase production from SMS as a feedstock was conducted using Central Composite Design (CCD). Cellulase was produced on SMS from P. pulmonarius via solid state fermentation using Trichoderma asperellum UPM 1. Significant parameters of temperature, moisture content, and pH employed at three different levels were highlighted and discussed. The optimum condition for maximum production of CMCase (171.21 U/g), FPase (9.85 U/g) and β-glucosidase (6.83 U/g) were as follows; temperature 27.5ºC, moisture content 81% and pH of fermentation 4.5. The optimized condition of cellulase production resulted in increment of 1.4-fold in CMCase and 1.5-fold in β-glucosidase with unoptimized condition produced 125.77 U/g CMCase and 4.67 U/g β-glucosidase. Mushrooms Mushroom culture 2016-08 Thesis http://psasir.upm.edu.my/id/eprint/75829/ http://psasir.upm.edu.my/id/eprint/75829/1/FBSB%202016%2044%20-%20IR.pdf text en public masters Universiti Putra Malaysia Mushrooms Mushroom culture
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Mushrooms Mushroom culture
spellingShingle	Mushrooms Mushroom culture Mohd Ariff, Iffah Nabilah Production of cellulolytic enzymes via solid state fermentation of spent mushroom substrate by Trichoderma asperellum UPM 1
description	Spent mushroom substrate (SMS) is a by-product generated after mushroom harvesting from the commercial mushroom cultivation industry. SMS had lost a significant fraction of their mass (approximately 35-50% depending on their nature) due to metabolic reactions, mushroom production and water evaporation. SMS generally composed of three main components i.e. fungal mycelia, extracellular enzymes secreted during growing of mushroom and unutilized lignocellulosic components. Delignified SMS with small particle size (<1 mm) has been observed to have high cellulose component and lignocellulolytic enzyme content. SMS comprised of high cellulose (45.5%) and partially degraded lignin by the action of Pleurotus pulmonarius during mushroom cultivation. SMS in readily available smaller particle size offers a great advantage as a fermentation feedstock. Hence, owing to this untapped potential, this study has investigated the feasibility of SMS as an alternative substrate for lignocellulolytic enzyme recovery. This was done by profiling ligninolytic enzymes activities produced during the growth of P. pulmonarius and P. floridanus. Crude laccase (3 U/g and 2.6 U/g) and MnP (1.4 U/g and 2.1 U/g) were detected in SMS from P. pulmonarius and P. floridanus, respectively. After enzyme recovery, optimization of cellulase production from SMS as a feedstock was conducted using Central Composite Design (CCD). Cellulase was produced on SMS from P. pulmonarius via solid state fermentation using Trichoderma asperellum UPM 1. Significant parameters of temperature, moisture content, and pH employed at three different levels were highlighted and discussed. The optimum condition for maximum production of CMCase (171.21 U/g), FPase (9.85 U/g) and β-glucosidase (6.83 U/g) were as follows; temperature 27.5ºC, moisture content 81% and pH of fermentation 4.5. The optimized condition of cellulase production resulted in increment of 1.4-fold in CMCase and 1.5-fold in β-glucosidase with unoptimized condition produced 125.77 U/g CMCase and 4.67 U/g β-glucosidase.
format	Thesis
qualification_level	Master's degree
author	Mohd Ariff, Iffah Nabilah
author_facet	Mohd Ariff, Iffah Nabilah
author_sort	Mohd Ariff, Iffah Nabilah
title	Production of cellulolytic enzymes via solid state fermentation of spent mushroom substrate by Trichoderma asperellum UPM 1
title_short	Production of cellulolytic enzymes via solid state fermentation of spent mushroom substrate by Trichoderma asperellum UPM 1
title_full	Production of cellulolytic enzymes via solid state fermentation of spent mushroom substrate by Trichoderma asperellum UPM 1
title_fullStr	Production of cellulolytic enzymes via solid state fermentation of spent mushroom substrate by Trichoderma asperellum UPM 1
title_full_unstemmed	Production of cellulolytic enzymes via solid state fermentation of spent mushroom substrate by Trichoderma asperellum UPM 1
title_sort	production of cellulolytic enzymes via solid state fermentation of spent mushroom substrate by trichoderma asperellum upm 1
granting_institution	Universiti Putra Malaysia
publishDate	2016
url	http://psasir.upm.edu.my/id/eprint/75829/1/FBSB%202016%2044%20-%20IR.pdf
_version_	1747813096050655232

Production of cellulolytic enzymes via solid state fermentation of spent mushroom substrate by Trichoderma asperellum UPM 1

Similar Items