Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential

Gut microbiota in vertebrate is complex and contains abundant of diverse beneficial microorganisms important for a dynamic host-microbe interaction. Some of these bacteria may have probiotics properties. In this study, the gut microbiota in wild and captive T. tambroides was identified through metag...

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Main Author:	Tan, Chun Keat
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Mahseers - Case studies - Malaysia Freshwater fishes - Malaysia Microbial ecology
Online Access:	http://psasir.upm.edu.my/id/eprint/76118/1/FP%202018%2053%20-%20IR.pdf
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id	my-upm-ir.76118
record_format	uketd_dc
spelling	my-upm-ir.761182019-12-02T01:56:29Z Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential 2017-12 Tan, Chun Keat Gut microbiota in vertebrate is complex and contains abundant of diverse beneficial microorganisms important for a dynamic host-microbe interaction. Some of these bacteria may have probiotics properties. In this study, the gut microbiota in wild and captive T. tambroides was identified through metagenetic sequencing of 16S rDNA V3-V4 hypervariable regions using Illumina MiSeq. The sequencing data were analyzed using Quantitative Insights into Microbial Ecology (QIIME). Bacteria were isolated from gut of T. tambroides to evaluate its safety and examined for its probiotics properties. These selected potential probiotics were fed to T. tambroides followed by challenge test to evaluate its effects on growth and disease resistance against Aeromonas hydrophila. The metagenetic analyses showed that the gut microbiota in T. tambroides was dominated by Firmicutes, Proteobacteria, Fusobacteria and Bacteroidetes. Wild T. tambroides gut contained Cetobacterium sp. (24.9%), unknown genus from Peprostreptococcaceae family (11.0%), Bacteroides sp. (10.1%), PSB-M-3 from Erysipelotrichaceae family (7.89%) and Vibrio sp. (5.4%). Captive T. tambroides gut contained Cetobacterium sp. (27.9%), Citrobacter sp. (10.0%), unknown genus from Peptostreptococcaceae family (8.2%), unknown genus from Aeromonadaceae family (8.2%) and Turicibacter sp. (7.0%). The results showed that Cetobacterium sp. is the core microbiota in T. tambroides gut. Function of this bacterium in T. tambroides gut needed to be determined. Three Aeromonas sp., two Bacillus sp., two Lysinibacillus spp., and one Pseudomonas sp. were successfully isolated and identified from the wild T. tambroides gut sample. Both Bacillus sp. and Pseudomonas sp. showed quorum sensing inhibition activities while only Pseudomonas sp. showed mild antimicrobial activity against A. hydrophila. These two bacteria were selected for probiotics feeding experiment. Nevertheless, there was no significant difference in growth of T. tambroides fed with these probiotics. The T. tambroides juveniles were then challenged with A. hydrophila by intra-peritoneal injection after the probiotics feeding experiment. Both Bacillus sp. and Pseudomonas sp. appeared to be able to improve disease resistance of T. tambroides juveniles against A. hydrophila infection. Lower mortality was observed in fishes treated with Bacillus sp. and Pseudomonas sp. as compared to positive control. Lysozyme activities in T. tambroides juveniles fed with Bacillus sp. were significantly higher (P<0.05) than other treatments. In conclusion, Cetobacterium sp. is a core gut microbiota in T. tambroides. The other important gut microbiota in T. tambroides may include Bacteroides sp., Citrobacter sp., Turicibacter sp. and Bacillus sp. Bacillus sp. and Pseudomonas sp. isolated in this study showed QSI activity. Bacillus sp. could enhance the innate immune system of fishes by increase lysozyme activity in blood serum. Both Bacillus sp. and Pseudomonas sp. have the potential to be use as probiotics in T. tambroides aquaculture to improve disease resistance and immune system of fishes. Mahseers - Case studies - Malaysia Freshwater fishes - Malaysia Microbial ecology 2017-12 Thesis http://psasir.upm.edu.my/id/eprint/76118/ http://psasir.upm.edu.my/id/eprint/76118/1/FP%202018%2053%20-%20IR.pdf text en public masters Universiti Putra Malaysia Mahseers - Case studies - Malaysia Freshwater fishes - Malaysia Microbial ecology
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Mahseers - Case studies - Malaysia Freshwater fishes - Malaysia Microbial ecology
spellingShingle	Mahseers - Case studies - Malaysia Freshwater fishes - Malaysia Microbial ecology Tan, Chun Keat Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
description	Gut microbiota in vertebrate is complex and contains abundant of diverse beneficial microorganisms important for a dynamic host-microbe interaction. Some of these bacteria may have probiotics properties. In this study, the gut microbiota in wild and captive T. tambroides was identified through metagenetic sequencing of 16S rDNA V3-V4 hypervariable regions using Illumina MiSeq. The sequencing data were analyzed using Quantitative Insights into Microbial Ecology (QIIME). Bacteria were isolated from gut of T. tambroides to evaluate its safety and examined for its probiotics properties. These selected potential probiotics were fed to T. tambroides followed by challenge test to evaluate its effects on growth and disease resistance against Aeromonas hydrophila. The metagenetic analyses showed that the gut microbiota in T. tambroides was dominated by Firmicutes, Proteobacteria, Fusobacteria and Bacteroidetes. Wild T. tambroides gut contained Cetobacterium sp. (24.9%), unknown genus from Peprostreptococcaceae family (11.0%), Bacteroides sp. (10.1%), PSB-M-3 from Erysipelotrichaceae family (7.89%) and Vibrio sp. (5.4%). Captive T. tambroides gut contained Cetobacterium sp. (27.9%), Citrobacter sp. (10.0%), unknown genus from Peptostreptococcaceae family (8.2%), unknown genus from Aeromonadaceae family (8.2%) and Turicibacter sp. (7.0%). The results showed that Cetobacterium sp. is the core microbiota in T. tambroides gut. Function of this bacterium in T. tambroides gut needed to be determined. Three Aeromonas sp., two Bacillus sp., two Lysinibacillus spp., and one Pseudomonas sp. were successfully isolated and identified from the wild T. tambroides gut sample. Both Bacillus sp. and Pseudomonas sp. showed quorum sensing inhibition activities while only Pseudomonas sp. showed mild antimicrobial activity against A. hydrophila. These two bacteria were selected for probiotics feeding experiment. Nevertheless, there was no significant difference in growth of T. tambroides fed with these probiotics. The T. tambroides juveniles were then challenged with A. hydrophila by intra-peritoneal injection after the probiotics feeding experiment. Both Bacillus sp. and Pseudomonas sp. appeared to be able to improve disease resistance of T. tambroides juveniles against A. hydrophila infection. Lower mortality was observed in fishes treated with Bacillus sp. and Pseudomonas sp. as compared to positive control. Lysozyme activities in T. tambroides juveniles fed with Bacillus sp. were significantly higher (P<0.05) than other treatments. In conclusion, Cetobacterium sp. is a core gut microbiota in T. tambroides. The other important gut microbiota in T. tambroides may include Bacteroides sp., Citrobacter sp., Turicibacter sp. and Bacillus sp. Bacillus sp. and Pseudomonas sp. isolated in this study showed QSI activity. Bacillus sp. could enhance the innate immune system of fishes by increase lysozyme activity in blood serum. Both Bacillus sp. and Pseudomonas sp. have the potential to be use as probiotics in T. tambroides aquaculture to improve disease resistance and immune system of fishes.
format	Thesis
qualification_level	Master's degree
author	Tan, Chun Keat
author_facet	Tan, Chun Keat
author_sort	Tan, Chun Keat
title	Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
title_short	Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
title_full	Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
title_fullStr	Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
title_full_unstemmed	Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
title_sort	metagenetic analysis of gut microbial community of malaysian mahseer tor tambroides (bleeker, 1854) (cyprinidae) and its probiotics potential
granting_institution	Universiti Putra Malaysia
publishDate	2017
url	http://psasir.upm.edu.my/id/eprint/76118/1/FP%202018%2053%20-%20IR.pdf
_version_	1747813122073165824

Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential

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