Anti-inflammatory effects of asiaticoside on tumor necrosis factor-α and interferon-ᵧ Induced raw 264.7 macrophage

Centella asiatica is one of the most commonly used medicinal herbs in traditional medicine. Its beneficial effects have been attributed to its major isolated triterpene, Asiaticoside. It has been described to exhibit anti-inflammatory activities in several models. Inflammatory cells produce large am...

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Main Author:	Zainudin, Nurfarahdilla
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Anti-Inflammatory Agents Tumor Necrosis Factor-alpha Centella
Online Access:	http://psasir.upm.edu.my/id/eprint/76280/1/FPSK%28M%29%202017%2074%20IR.pdf
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id	my-upm-ir.76280
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spelling	my-upm-ir.762802019-11-27T07:45:02Z Anti-inflammatory effects of asiaticoside on tumor necrosis factor-α and interferon-ᵧ Induced raw 264.7 macrophage 2016-11 Zainudin, Nurfarahdilla Centella asiatica is one of the most commonly used medicinal herbs in traditional medicine. Its beneficial effects have been attributed to its major isolated triterpene, Asiaticoside. It has been described to exhibit anti-inflammatory activities in several models. Inflammatory cells produce large amount of nitric oxide, prostaglandin E2 and cyclooxygenase. These activated macrophages functions as effectors molecules and are involved in physiological and pathological responses in neural, vascular and immune system. In this study, tumor necrosis factor-α and interferon-γ-induced inflammatory response was introduced in RAW 264.7macrophages cells to evaluate the potential effects of Asiaticoside (1.25 μM-50 μM) in these cells. Quantification of nitric oxide was determined through the Griess reaction by a microplate assay method. Prostaglandin E2 and cyclooxygenase 2 releases were quantified by EIA. To detect whether the effect of Asiaticoside on tumor necrosis factor-α and interferon-γ-stimulated RAW 264.7 macrophages cells occurred via the mitogen-activated protein kinase signal transduction pathways, western blot was performed. The results showed that Asiaticoside significantly inhibited tumor necrosis factor-α and interferon-γ -induced nitric oxide, prostaglandin E2 and cyclooxygenase-2 productions which is mediated by the suppression of the p38 mitogen-activated protein kinase signaling pathway. Based on our current findings, we concluded that Asiaticoside has anti-inflammatory effects and may be used as a therapeutic agent for the treatment of inflammation. Anti-Inflammatory Agents Tumor Necrosis Factor-alpha Centella 2016-11 Thesis http://psasir.upm.edu.my/id/eprint/76280/ http://psasir.upm.edu.my/id/eprint/76280/1/FPSK%28M%29%202017%2074%20IR.pdf text en public masters Universiti Putra Malaysia Anti-Inflammatory Agents Tumor Necrosis Factor-alpha Centella
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Anti-Inflammatory Agents Tumor Necrosis Factor-alpha Centella
spellingShingle	Anti-Inflammatory Agents Tumor Necrosis Factor-alpha Centella Zainudin, Nurfarahdilla Anti-inflammatory effects of asiaticoside on tumor necrosis factor-α and interferon-ᵧ Induced raw 264.7 macrophage
description	Centella asiatica is one of the most commonly used medicinal herbs in traditional medicine. Its beneficial effects have been attributed to its major isolated triterpene, Asiaticoside. It has been described to exhibit anti-inflammatory activities in several models. Inflammatory cells produce large amount of nitric oxide, prostaglandin E2 and cyclooxygenase. These activated macrophages functions as effectors molecules and are involved in physiological and pathological responses in neural, vascular and immune system. In this study, tumor necrosis factor-α and interferon-γ-induced inflammatory response was introduced in RAW 264.7macrophages cells to evaluate the potential effects of Asiaticoside (1.25 μM-50 μM) in these cells. Quantification of nitric oxide was determined through the Griess reaction by a microplate assay method. Prostaglandin E2 and cyclooxygenase 2 releases were quantified by EIA. To detect whether the effect of Asiaticoside on tumor necrosis factor-α and interferon-γ-stimulated RAW 264.7 macrophages cells occurred via the mitogen-activated protein kinase signal transduction pathways, western blot was performed. The results showed that Asiaticoside significantly inhibited tumor necrosis factor-α and interferon-γ -induced nitric oxide, prostaglandin E2 and cyclooxygenase-2 productions which is mediated by the suppression of the p38 mitogen-activated protein kinase signaling pathway. Based on our current findings, we concluded that Asiaticoside has anti-inflammatory effects and may be used as a therapeutic agent for the treatment of inflammation.
format	Thesis
qualification_level	Master's degree
author	Zainudin, Nurfarahdilla
author_facet	Zainudin, Nurfarahdilla
author_sort	Zainudin, Nurfarahdilla
title	Anti-inflammatory effects of asiaticoside on tumor necrosis factor-α and interferon-ᵧ Induced raw 264.7 macrophage
title_short	Anti-inflammatory effects of asiaticoside on tumor necrosis factor-α and interferon-ᵧ Induced raw 264.7 macrophage
title_full	Anti-inflammatory effects of asiaticoside on tumor necrosis factor-α and interferon-ᵧ Induced raw 264.7 macrophage
title_fullStr	Anti-inflammatory effects of asiaticoside on tumor necrosis factor-α and interferon-ᵧ Induced raw 264.7 macrophage
title_full_unstemmed	Anti-inflammatory effects of asiaticoside on tumor necrosis factor-α and interferon-ᵧ Induced raw 264.7 macrophage
title_sort	anti-inflammatory effects of asiaticoside on tumor necrosis factor-α and interferon-ᵧ induced raw 264.7 macrophage
granting_institution	Universiti Putra Malaysia
publishDate	2016
url	http://psasir.upm.edu.my/id/eprint/76280/1/FPSK%28M%29%202017%2074%20IR.pdf
_version_	1747813147427733504

Anti-inflammatory effects of asiaticoside on tumor necrosis factor-α and interferon-ᵧ Induced raw 264.7 macrophage

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