Efficacy of betel leaves, Piper betle L. crude extract on Vibrio alginolyticus in cultured Asian seabass, Lates calcarifer (Bloch, 1790)

Production of Asian seabass, Lates calcarifer is facing a crucial problem due to vibriosis caused by Vibrio alginolyticus. The current treatment using commercial antibiotic is not encouraged due to the issue of antibiotic resistant bacteria. Betel leaf, Piper betle has been known to contain various...

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Main Author:	Othman, Ahmad Baihaqi
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Giant perch - Research Betel leaves
Online Access:	http://psasir.upm.edu.my/id/eprint/76323/1/FPV%202018%2033%20IR.pdf
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id	my-upm-ir.76323
record_format	uketd_dc
spelling	my-upm-ir.763232020-01-21T07:09:12Z Efficacy of betel leaves, Piper betle L. crude extract on Vibrio alginolyticus in cultured Asian seabass, Lates calcarifer (Bloch, 1790) 2018-05 Othman, Ahmad Baihaqi Production of Asian seabass, Lates calcarifer is facing a crucial problem due to vibriosis caused by Vibrio alginolyticus. The current treatment using commercial antibiotic is not encouraged due to the issue of antibiotic resistant bacteria. Betel leaf, Piper betle has been known to contain various active compounds that are effective against bacterial infection and maintain the fish health. This study used several compounds for extraction medium of the active compounds of betel leaf. Ethanol has been concluded as the best solvent for the extraction of the active ingredients of betel leaf. The ethanoic crude extract showed inhibition zone of 21 mm compared with aques extraction with 9 mm (p<0.05). Results of the minimum inhibitory concentration (MIC) test indicate that the extract could inhibit Vibrio alginolyticus at a concentration of 0.2 mg/mL and could kill the bacteria at concentration of 0.39 mg/mL (MBC). The palatability test revealed that the seabass could consume 100% of the crude extract as high as 200mg/kg of feed for over 7 days without showing abnormality. Next, the challenge test was done in two trials to test the crude extract as preventive and as treatment against vibriosis due to Vibrio alginolyticus. For the preventive test, the fish were fed with crude extract at 100mg/kg of feed before being infected with Vibrio alginolyticus. For the treatment test, the fish were initially infected with Vibrio alginolyticus before being treated with crude extract at the same dose of 100mg/kg of feed. The results revealed that both preventive and treatment trials produced significantly (p<0.05) higher survival rate compared with oxytetracycline. These results showed that the betel leaf crude extract is highly potential to be used as preventive measure against vibriosis. Giant perch - Research Betel leaves 2018-05 Thesis http://psasir.upm.edu.my/id/eprint/76323/ http://psasir.upm.edu.my/id/eprint/76323/1/FPV%202018%2033%20IR.pdf text en public masters Universiti Putra Malaysia Giant perch - Research Betel leaves
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Giant perch - Research Betel leaves
spellingShingle	Giant perch - Research Betel leaves Othman, Ahmad Baihaqi Efficacy of betel leaves, Piper betle L. crude extract on Vibrio alginolyticus in cultured Asian seabass, Lates calcarifer (Bloch, 1790)
description	Production of Asian seabass, Lates calcarifer is facing a crucial problem due to vibriosis caused by Vibrio alginolyticus. The current treatment using commercial antibiotic is not encouraged due to the issue of antibiotic resistant bacteria. Betel leaf, Piper betle has been known to contain various active compounds that are effective against bacterial infection and maintain the fish health. This study used several compounds for extraction medium of the active compounds of betel leaf. Ethanol has been concluded as the best solvent for the extraction of the active ingredients of betel leaf. The ethanoic crude extract showed inhibition zone of 21 mm compared with aques extraction with 9 mm (p<0.05). Results of the minimum inhibitory concentration (MIC) test indicate that the extract could inhibit Vibrio alginolyticus at a concentration of 0.2 mg/mL and could kill the bacteria at concentration of 0.39 mg/mL (MBC). The palatability test revealed that the seabass could consume 100% of the crude extract as high as 200mg/kg of feed for over 7 days without showing abnormality. Next, the challenge test was done in two trials to test the crude extract as preventive and as treatment against vibriosis due to Vibrio alginolyticus. For the preventive test, the fish were fed with crude extract at 100mg/kg of feed before being infected with Vibrio alginolyticus. For the treatment test, the fish were initially infected with Vibrio alginolyticus before being treated with crude extract at the same dose of 100mg/kg of feed. The results revealed that both preventive and treatment trials produced significantly (p<0.05) higher survival rate compared with oxytetracycline. These results showed that the betel leaf crude extract is highly potential to be used as preventive measure against vibriosis.
format	Thesis
qualification_level	Master's degree
author	Othman, Ahmad Baihaqi
author_facet	Othman, Ahmad Baihaqi
author_sort	Othman, Ahmad Baihaqi
title	Efficacy of betel leaves, Piper betle L. crude extract on Vibrio alginolyticus in cultured Asian seabass, Lates calcarifer (Bloch, 1790)
title_short	Efficacy of betel leaves, Piper betle L. crude extract on Vibrio alginolyticus in cultured Asian seabass, Lates calcarifer (Bloch, 1790)
title_full	Efficacy of betel leaves, Piper betle L. crude extract on Vibrio alginolyticus in cultured Asian seabass, Lates calcarifer (Bloch, 1790)
title_fullStr	Efficacy of betel leaves, Piper betle L. crude extract on Vibrio alginolyticus in cultured Asian seabass, Lates calcarifer (Bloch, 1790)
title_full_unstemmed	Efficacy of betel leaves, Piper betle L. crude extract on Vibrio alginolyticus in cultured Asian seabass, Lates calcarifer (Bloch, 1790)
title_sort	efficacy of betel leaves, piper betle l. crude extract on vibrio alginolyticus in cultured asian seabass, lates calcarifer (bloch, 1790)
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/76323/1/FPV%202018%2033%20IR.pdf
_version_	1747813155818438656

Efficacy of betel leaves, Piper betle L. crude extract on Vibrio alginolyticus in cultured Asian seabass, Lates calcarifer (Bloch, 1790)

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