Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia

Coagulase-negative staphylococci (CoNS) are often considered as contaminants or normal skin flora but in the past few decades, they had emerged as pathogenic bacteria causing serious infections especially in patients with prosthetic medical devices. Their ability to produce biofilm on polymer...

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Main Author:	Vnayakam, Subashini
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Staphylococcal Infections Coagulase
Online Access:	http://psasir.upm.edu.my/id/eprint/76692/1/FPSK%28M%29%202018%2049%20%20IR.pdf
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id	my-upm-ir.76692
record_format	uketd_dc
spelling	my-upm-ir.766922020-02-03T01:52:04Z Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia 2017-10 Vnayakam, Subashini Coagulase-negative staphylococci (CoNS) are often considered as contaminants or normal skin flora but in the past few decades, they had emerged as pathogenic bacteria causing serious infections especially in patients with prosthetic medical devices. Their ability to produce biofilm on polymer surfaces results in treatment failure as it increases the resistance to antibiotics and host defense. Biofilms formation are mediated by polysaccharide intercellular adhesin (PIA), encoded by intercellular adhesion (ica) genes and it has been postulated that the presence of ica genes in CoNS is associated with antimicrobial resistance and responsible for catheter and medical device-related sepsis. The aim of this study is to determine the species distribution, antibiotic susceptibility pattern and to detect the presence of ica genes (icaA, icaD, icaB, and icaC) among CoNS isolated from blood culture. This cross-sectional study was conducted between January 2015 and December 2015, with CoNS isolates obtained from the blood cultures of patients at Hospital Serdang. Coagulase negative staphylococci were identified by gram staining, catalase and coagulase test, followed by species identification by Analytical Profile Index (API) Staph identification strips. Antimicrobial susceptibility testing was performed using Kirby Bauer method interpreted according to the Clinical and Laboratory Standards Institute guidelines. The presence of ica genes were detected using multiplex polymerase chain reaction. Staphylococcus epidermidis dominated the total number of species isolated (n=64, 40.0%). A total of 160 CoNS, 72.5% were resistant to penicillin and 60% were methicillin-resistant (MR) CoNS. Majority of CoNS harbored icaD (59.3%) and in 4 (2.5%) strains, all icaADBC genes were observed. Detection of ica genes indicates CoNS are able to produce biofilm that causes serious nosocomial infections and should not always be reported as colonizer or contaminants. Staphylococcal Infections Coagulase 2017-10 Thesis http://psasir.upm.edu.my/id/eprint/76692/ http://psasir.upm.edu.my/id/eprint/76692/1/FPSK%28M%29%202018%2049%20%20IR.pdf text en public masters Universiti Putra Malaysia Staphylococcal Infections Coagulase
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Staphylococcal Infections Coagulase
spellingShingle	Staphylococcal Infections Coagulase Vnayakam, Subashini Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia
description	Coagulase-negative staphylococci (CoNS) are often considered as contaminants or normal skin flora but in the past few decades, they had emerged as pathogenic bacteria causing serious infections especially in patients with prosthetic medical devices. Their ability to produce biofilm on polymer surfaces results in treatment failure as it increases the resistance to antibiotics and host defense. Biofilms formation are mediated by polysaccharide intercellular adhesin (PIA), encoded by intercellular adhesion (ica) genes and it has been postulated that the presence of ica genes in CoNS is associated with antimicrobial resistance and responsible for catheter and medical device-related sepsis. The aim of this study is to determine the species distribution, antibiotic susceptibility pattern and to detect the presence of ica genes (icaA, icaD, icaB, and icaC) among CoNS isolated from blood culture. This cross-sectional study was conducted between January 2015 and December 2015, with CoNS isolates obtained from the blood cultures of patients at Hospital Serdang. Coagulase negative staphylococci were identified by gram staining, catalase and coagulase test, followed by species identification by Analytical Profile Index (API) Staph identification strips. Antimicrobial susceptibility testing was performed using Kirby Bauer method interpreted according to the Clinical and Laboratory Standards Institute guidelines. The presence of ica genes were detected using multiplex polymerase chain reaction. Staphylococcus epidermidis dominated the total number of species isolated (n=64, 40.0%). A total of 160 CoNS, 72.5% were resistant to penicillin and 60% were methicillin-resistant (MR) CoNS. Majority of CoNS harbored icaD (59.3%) and in 4 (2.5%) strains, all icaADBC genes were observed. Detection of ica genes indicates CoNS are able to produce biofilm that causes serious nosocomial infections and should not always be reported as colonizer or contaminants.
format	Thesis
qualification_level	Master's degree
author	Vnayakam, Subashini
author_facet	Vnayakam, Subashini
author_sort	Vnayakam, Subashini
title	Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia
title_short	Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia
title_full	Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia
title_fullStr	Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia
title_full_unstemmed	Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia
title_sort	characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in malaysia
granting_institution	Universiti Putra Malaysia
publishDate	2017
url	http://psasir.upm.edu.my/id/eprint/76692/1/FPSK%28M%29%202018%2049%20%20IR.pdf
_version_	1747813175514890240

Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia

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