Surface deposition of zinc oxide/vancomycin for enantioselective electrochemical sensing of penicillamine enantiomers

The development of chiral electrochemical sensor in the recognition of Penicillamine (Pen) enantiomer is important for the production of pure enantiomers over racemate in the treatment of an array of diseases. The preparation of the modified electrode was a twofold procedure in which electrodepos...

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Main Author:	Lim, Alvin Teik Zheng
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Electrophoretic deposition Zinc oxide Electrochemical analysis
Online Access:	http://psasir.upm.edu.my/id/eprint/76763/1/FS%202018%2066%20-%20IR.pdf
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spelling	my-upm-ir.767632020-02-10T07:51:30Z Surface deposition of zinc oxide/vancomycin for enantioselective electrochemical sensing of penicillamine enantiomers 2018-01 Lim, Alvin Teik Zheng The development of chiral electrochemical sensor in the recognition of Penicillamine (Pen) enantiomer is important for the production of pure enantiomers over racemate in the treatment of an array of diseases. The preparation of the modified electrode was a twofold procedure in which electrodeposition of ZnO was conducted first on the ITO glass substrate. The optimum deposition parameters were at –0.9 V, in a buffer solution of pH 7 at 60 oC for 900 s based on the cyclic voltammetry (CV) data obtained. The second part of the procedure involved dropcasting Van solution on the surface of the ZnO/ITO modified electrode. Electrochemical impedance spectroscopy (EIS) were carried out to characterize the electrical conductivity of the modified electrodes.. In this study, a novel chiral electrochemical sensor based on ZnO/vancomycin modified indium tin oxide glass substrate (ZnO/Van/ITO) was successfully fabricated and used in the chiral recognition of Pen enantiomers. The results showed that the charge transfer resistance (RCT) is the greatest for ZnO/Van/ITO followed by ZnO/ITO, Van/ITO and bare ITO. Field Emission Scanning Electron Microscopy was carried out to study the surface morphology of the modified electrodes surface. Different oxidation peak current, Ip of L- and D- Pen were observed in the differential pulse voltammograms (DPV) obtained in the solution containing L- or D-Pen. Under optimum condition, the chiral sensor exhibited good linear response to Pen enantiomers in a linear range of 5 mM to 30 mM with a detection limit of 23.56 mM and 14.73 mM (S/N=3) for the D-Pen and L-Pen respectively. This proposed electrode was shown to exhibit excellent performance in terms of low detection limit and good enantioselectivity for Pen enantiomers. Molecular docking using PyRx was further used to study the computational binding effect of the Van and Pen enantiomers. The results showed that the binding effect was more prevalent for D-Pen as compared to L-Pen with a value of -2.3 kcal/mol and -2.4 kcal/mol for the D-Pen and L-Pen respectively. The association constant from the experiment, KD and KL obtained were 2.314 × 103 and 1.076 ×103 L/mol for the D and L-Pen respectively. The value of KD was larger compared to KL indicating that the binding effect is higher between Van/ZnO with D-Pen. The results obtained suggested that this chiral sensor has the ability to discriminate chiral drugs which is useful in pharmaceutical industries. Electrophoretic deposition Zinc oxide Electrochemical analysis 2018-01 Thesis http://psasir.upm.edu.my/id/eprint/76763/ http://psasir.upm.edu.my/id/eprint/76763/1/FS%202018%2066%20-%20IR.pdf text en public masters Universiti Putra Malaysia Electrophoretic deposition Zinc oxide Electrochemical analysis
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Electrophoretic deposition Zinc oxide Electrochemical analysis
spellingShingle	Electrophoretic deposition Zinc oxide Electrochemical analysis Lim, Alvin Teik Zheng Surface deposition of zinc oxide/vancomycin for enantioselective electrochemical sensing of penicillamine enantiomers
description	The development of chiral electrochemical sensor in the recognition of Penicillamine (Pen) enantiomer is important for the production of pure enantiomers over racemate in the treatment of an array of diseases. The preparation of the modified electrode was a twofold procedure in which electrodeposition of ZnO was conducted first on the ITO glass substrate. The optimum deposition parameters were at –0.9 V, in a buffer solution of pH 7 at 60 oC for 900 s based on the cyclic voltammetry (CV) data obtained. The second part of the procedure involved dropcasting Van solution on the surface of the ZnO/ITO modified electrode. Electrochemical impedance spectroscopy (EIS) were carried out to characterize the electrical conductivity of the modified electrodes.. In this study, a novel chiral electrochemical sensor based on ZnO/vancomycin modified indium tin oxide glass substrate (ZnO/Van/ITO) was successfully fabricated and used in the chiral recognition of Pen enantiomers. The results showed that the charge transfer resistance (RCT) is the greatest for ZnO/Van/ITO followed by ZnO/ITO, Van/ITO and bare ITO. Field Emission Scanning Electron Microscopy was carried out to study the surface morphology of the modified electrodes surface. Different oxidation peak current, Ip of L- and D- Pen were observed in the differential pulse voltammograms (DPV) obtained in the solution containing L- or D-Pen. Under optimum condition, the chiral sensor exhibited good linear response to Pen enantiomers in a linear range of 5 mM to 30 mM with a detection limit of 23.56 mM and 14.73 mM (S/N=3) for the D-Pen and L-Pen respectively. This proposed electrode was shown to exhibit excellent performance in terms of low detection limit and good enantioselectivity for Pen enantiomers. Molecular docking using PyRx was further used to study the computational binding effect of the Van and Pen enantiomers. The results showed that the binding effect was more prevalent for D-Pen as compared to L-Pen with a value of -2.3 kcal/mol and -2.4 kcal/mol for the D-Pen and L-Pen respectively. The association constant from the experiment, KD and KL obtained were 2.314 × 103 and 1.076 ×103 L/mol for the D and L-Pen respectively. The value of KD was larger compared to KL indicating that the binding effect is higher between Van/ZnO with D-Pen. The results obtained suggested that this chiral sensor has the ability to discriminate chiral drugs which is useful in pharmaceutical industries.
format	Thesis
qualification_level	Master's degree
author	Lim, Alvin Teik Zheng
author_facet	Lim, Alvin Teik Zheng
author_sort	Lim, Alvin Teik Zheng
title	Surface deposition of zinc oxide/vancomycin for enantioselective electrochemical sensing of penicillamine enantiomers
title_short	Surface deposition of zinc oxide/vancomycin for enantioselective electrochemical sensing of penicillamine enantiomers
title_full	Surface deposition of zinc oxide/vancomycin for enantioselective electrochemical sensing of penicillamine enantiomers
title_fullStr	Surface deposition of zinc oxide/vancomycin for enantioselective electrochemical sensing of penicillamine enantiomers
title_full_unstemmed	Surface deposition of zinc oxide/vancomycin for enantioselective electrochemical sensing of penicillamine enantiomers
title_sort	surface deposition of zinc oxide/vancomycin for enantioselective electrochemical sensing of penicillamine enantiomers
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/76763/1/FS%202018%2066%20-%20IR.pdf
_version_	1747813178957365248

Surface deposition of zinc oxide/vancomycin for enantioselective electrochemical sensing of penicillamine enantiomers

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