Optimization study on antioxidant in fruit extract of Phaleria macrocarpa (Scheff.) Boerl in Malaysia and evaluation on its functional cosmetic

Phaleria macrocarpa (Scheff.) Boerl belong to Thymelaeceae family and widely known as ‘Crown of God’, ‘Mahkota Dewa’ and ‘Pau’. It is a native plant from the Papua Island, Indonesia and grows in tropical areas. It had been reported P.macrocarpa have traditional medicine and bioactive activitie...

Full description

Saved in:

Bibliographic Details
Main Author:	Mohamed Mahzir, Khurul Ain
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Thymelaeaceae Flavonoids
Online Access:	http://psasir.upm.edu.my/id/eprint/76972/1/IPPH%202018%202%20-%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.76972
record_format	uketd_dc
spelling	my-upm-ir.769722020-02-10T00:19:42Z Optimization study on antioxidant in fruit extract of Phaleria macrocarpa (Scheff.) Boerl in Malaysia and evaluation on its functional cosmetic 2018-01 Mohamed Mahzir, Khurul Ain Phaleria macrocarpa (Scheff.) Boerl belong to Thymelaeceae family and widely known as ‘Crown of God’, ‘Mahkota Dewa’ and ‘Pau’. It is a native plant from the Papua Island, Indonesia and grows in tropical areas. It had been reported P.macrocarpa have traditional medicine and bioactive activities such as anti-tumor, anti-hyperglycemia, anti-inflammation, anti-diarrhoel, anti-oxidant, anti-viral, antibacterial, anti-fungal and vasodilator effect. However, there is still lacking in investigation of antioxidants by using Response Surface Methodology (RSM). Therefore,in this study to determine the antioxidants properties such as [2,2- diphenly-1-picrylhydrazyl (DPPH) free radical scavenging activity, total phenolic content (TPC), total flavonoid content (TFC) and ferric ion reducing power assay (FRAP) of P.macrocarpa fruits by using Response Surface Methodology, Central Composite Design (CCD) was applied to determine the maximum percentages yield by focusing on three independent variables which include extraction temperature (53.18- ْ 80 C), extraction time (66.30-133.64 minutes) and solvent ethanol :water ratio (63.18-96.82%v/v) with twenty experiments was carried out. The extraction was carried out manually by using reflux set apparatus and filter out the solvent manually. The optimal conditions for the antioxidants extraction were found to be extraction temperature (63.60 ºC), extraction time (66.36 minutes) and solvent of ethanol:water ratio (74.36 %v/v) with the highest percentage yield of DPPH, TPC, TFC and FRAP were 87.23%, 268.03&, 3.22% and 7.49%. Therefore, the yield of antioxidants activities obtained experimentally was close to the predicted values and suitability of the model employed in RSM to optimizing the extraction condition. In a conclusion, RSM was a good statistical tool with empower evaluation of the effects of certain process variables and their interaction of response variables in a short time, less cost and less number of required experiments. Thymelaeaceae Flavonoids 2018-01 Thesis http://psasir.upm.edu.my/id/eprint/76972/ http://psasir.upm.edu.my/id/eprint/76972/1/IPPH%202018%202%20-%20IR.pdf text en public masters Universiti Putra Malaysia Thymelaeaceae Flavonoids Abd Gani, Siti Salwa
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Abd Gani, Siti Salwa
topic	Thymelaeaceae Flavonoids
spellingShingle	Thymelaeaceae Flavonoids Mohamed Mahzir, Khurul Ain Optimization study on antioxidant in fruit extract of Phaleria macrocarpa (Scheff.) Boerl in Malaysia and evaluation on its functional cosmetic
description	Phaleria macrocarpa (Scheff.) Boerl belong to Thymelaeceae family and widely known as ‘Crown of God’, ‘Mahkota Dewa’ and ‘Pau’. It is a native plant from the Papua Island, Indonesia and grows in tropical areas. It had been reported P.macrocarpa have traditional medicine and bioactive activities such as anti-tumor, anti-hyperglycemia, anti-inflammation, anti-diarrhoel, anti-oxidant, anti-viral, antibacterial, anti-fungal and vasodilator effect. However, there is still lacking in investigation of antioxidants by using Response Surface Methodology (RSM). Therefore,in this study to determine the antioxidants properties such as [2,2- diphenly-1-picrylhydrazyl (DPPH) free radical scavenging activity, total phenolic content (TPC), total flavonoid content (TFC) and ferric ion reducing power assay (FRAP) of P.macrocarpa fruits by using Response Surface Methodology, Central Composite Design (CCD) was applied to determine the maximum percentages yield by focusing on three independent variables which include extraction temperature (53.18- ْ 80 C), extraction time (66.30-133.64 minutes) and solvent ethanol :water ratio (63.18-96.82%v/v) with twenty experiments was carried out. The extraction was carried out manually by using reflux set apparatus and filter out the solvent manually. The optimal conditions for the antioxidants extraction were found to be extraction temperature (63.60 ºC), extraction time (66.36 minutes) and solvent of ethanol:water ratio (74.36 %v/v) with the highest percentage yield of DPPH, TPC, TFC and FRAP were 87.23%, 268.03&, 3.22% and 7.49%. Therefore, the yield of antioxidants activities obtained experimentally was close to the predicted values and suitability of the model employed in RSM to optimizing the extraction condition. In a conclusion, RSM was a good statistical tool with empower evaluation of the effects of certain process variables and their interaction of response variables in a short time, less cost and less number of required experiments.
format	Thesis
qualification_level	Master's degree
author	Mohamed Mahzir, Khurul Ain
author_facet	Mohamed Mahzir, Khurul Ain
author_sort	Mohamed Mahzir, Khurul Ain
title	Optimization study on antioxidant in fruit extract of Phaleria macrocarpa (Scheff.) Boerl in Malaysia and evaluation on its functional cosmetic
title_short	Optimization study on antioxidant in fruit extract of Phaleria macrocarpa (Scheff.) Boerl in Malaysia and evaluation on its functional cosmetic
title_full	Optimization study on antioxidant in fruit extract of Phaleria macrocarpa (Scheff.) Boerl in Malaysia and evaluation on its functional cosmetic
title_fullStr	Optimization study on antioxidant in fruit extract of Phaleria macrocarpa (Scheff.) Boerl in Malaysia and evaluation on its functional cosmetic
title_full_unstemmed	Optimization study on antioxidant in fruit extract of Phaleria macrocarpa (Scheff.) Boerl in Malaysia and evaluation on its functional cosmetic
title_sort	optimization study on antioxidant in fruit extract of phaleria macrocarpa (scheff.) boerl in malaysia and evaluation on its functional cosmetic
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/76972/1/IPPH%202018%202%20-%20IR.pdf
_version_	1747813195392745472

Optimization study on antioxidant in fruit extract of Phaleria macrocarpa (Scheff.) Boerl in Malaysia and evaluation on its functional cosmetic

Similar Items