Prevalence and antibiogram of Staphylococcus aureus isolated from a research laboratory, its transmission and cross contamination to fruits and kitchen utensils

Staphylococcus aureus is an important foodborne pathogen found on the skin and in the nose of a healthy person as normal flora. S. aureus can be acquired and transmitted from contaminated hands to fruits and kitchen utensils. The objectives of this research were to determine the prevalence and antib...

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Main Author:	Eden, Vitalis Ronald
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Staphylococcus aureus Molecular biology
Online Access:	http://psasir.upm.edu.my/id/eprint/77767/1/FSTM%202018%2033%20ir.pdf
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id	my-upm-ir.77767
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spelling	my-upm-ir.777672022-01-21T08:08:24Z Prevalence and antibiogram of Staphylococcus aureus isolated from a research laboratory, its transmission and cross contamination to fruits and kitchen utensils 2018-06 Eden, Vitalis Ronald Staphylococcus aureus is an important foodborne pathogen found on the skin and in the nose of a healthy person as normal flora. S. aureus can be acquired and transmitted from contaminated hands to fruits and kitchen utensils. The objectives of this research were to determine the prevalence and antibiogram of S. aureus in microbiology research laboratory, transmission and its cross contamination to fruits and kitchen utensils. A total of 320 isolates from nose=80; hands=80; door knobs=80 and table surface=80 were taken from microbiology research laboratory in Petaling Jaya, Selangor were examined for the presence of S. aureus using Most Probable Number method. The strain of S. aureus obtained were used in simulation test using cutting board=15; knife=15; fork=15; table cloth=15; and watermelon=15. The prevalence of S. aureus estimated in microbiology research laboratory was 81.56% whereby 75% before work and 88.12% after work ranged from 3.6 MPN/mL to 140 MPN/mL. In simulation test, it was identified that S. aureus can be transmitted via contaminated hands to fruits and kitchen utensils during food preparation whereby about 65.3% of S. aureus obtained from 75 isolates and watermelon recorded the highest load of S. aureus about 86.6%. Meanwhile in antibiogram test, S. aureus were found resistant to oxacillin (85.4%) and gentamicin (74.3%). The Actor Network Theory applied in this study identified that the strains of S. aureus obtained in microbiology research laboratory were able to contaminate the fruits and kitchen utensils during food preparation. Furthermore, Risk Ranger estimated that the risk of acquiring S. aureus were moderate with 4 illnesses predicted per annum scoring about 83/100. Hence, contemporary healthcare strategies must be taken immediately to prevent the transmission of S. aureus in microbiology research laboratory and its cross contamination to fruits and kitchen utensils. Staphylococcus aureus Molecular biology 2018-06 Thesis http://psasir.upm.edu.my/id/eprint/77767/ http://psasir.upm.edu.my/id/eprint/77767/1/FSTM%202018%2033%20ir.pdf text en public masters Universiti Putra Malaysia Staphylococcus aureus Molecular biology Radu, Son
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Radu, Son
topic	Staphylococcus aureus Molecular biology
spellingShingle	Staphylococcus aureus Molecular biology Eden, Vitalis Ronald Prevalence and antibiogram of Staphylococcus aureus isolated from a research laboratory, its transmission and cross contamination to fruits and kitchen utensils
description	Staphylococcus aureus is an important foodborne pathogen found on the skin and in the nose of a healthy person as normal flora. S. aureus can be acquired and transmitted from contaminated hands to fruits and kitchen utensils. The objectives of this research were to determine the prevalence and antibiogram of S. aureus in microbiology research laboratory, transmission and its cross contamination to fruits and kitchen utensils. A total of 320 isolates from nose=80; hands=80; door knobs=80 and table surface=80 were taken from microbiology research laboratory in Petaling Jaya, Selangor were examined for the presence of S. aureus using Most Probable Number method. The strain of S. aureus obtained were used in simulation test using cutting board=15; knife=15; fork=15; table cloth=15; and watermelon=15. The prevalence of S. aureus estimated in microbiology research laboratory was 81.56% whereby 75% before work and 88.12% after work ranged from 3.6 MPN/mL to 140 MPN/mL. In simulation test, it was identified that S. aureus can be transmitted via contaminated hands to fruits and kitchen utensils during food preparation whereby about 65.3% of S. aureus obtained from 75 isolates and watermelon recorded the highest load of S. aureus about 86.6%. Meanwhile in antibiogram test, S. aureus were found resistant to oxacillin (85.4%) and gentamicin (74.3%). The Actor Network Theory applied in this study identified that the strains of S. aureus obtained in microbiology research laboratory were able to contaminate the fruits and kitchen utensils during food preparation. Furthermore, Risk Ranger estimated that the risk of acquiring S. aureus were moderate with 4 illnesses predicted per annum scoring about 83/100. Hence, contemporary healthcare strategies must be taken immediately to prevent the transmission of S. aureus in microbiology research laboratory and its cross contamination to fruits and kitchen utensils.
format	Thesis
qualification_level	Master's degree
author	Eden, Vitalis Ronald
author_facet	Eden, Vitalis Ronald
author_sort	Eden, Vitalis Ronald
title	Prevalence and antibiogram of Staphylococcus aureus isolated from a research laboratory, its transmission and cross contamination to fruits and kitchen utensils
title_short	Prevalence and antibiogram of Staphylococcus aureus isolated from a research laboratory, its transmission and cross contamination to fruits and kitchen utensils
title_full	Prevalence and antibiogram of Staphylococcus aureus isolated from a research laboratory, its transmission and cross contamination to fruits and kitchen utensils
title_fullStr	Prevalence and antibiogram of Staphylococcus aureus isolated from a research laboratory, its transmission and cross contamination to fruits and kitchen utensils
title_full_unstemmed	Prevalence and antibiogram of Staphylococcus aureus isolated from a research laboratory, its transmission and cross contamination to fruits and kitchen utensils
title_sort	prevalence and antibiogram of staphylococcus aureus isolated from a research laboratory, its transmission and cross contamination to fruits and kitchen utensils
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/77767/1/FSTM%202018%2033%20ir.pdf
_version_	1747813258839982080

Prevalence and antibiogram of Staphylococcus aureus isolated from a research laboratory, its transmission and cross contamination to fruits and kitchen utensils

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