Presence of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in local beef and Indian buffalo meat

Shiga-like toxin producing Escherichia coli bacteria are well known to be the pathogenic bacteria that cause traveller diarrhea. E. coli O157: H7 from the group of Shiga-like toxin producing E. coli cause even severe infection which can lead to fatal in human. In this study, local beef and India buf...

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Main Author:	Kwan, Sin Yen
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Escherichia coli Foodborne diseases
Online Access:	http://psasir.upm.edu.my/id/eprint/77782/1/FSTM%202019%207%20ir.pdf
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id	my-upm-ir.77782
record_format	uketd_dc
spelling	my-upm-ir.777822022-01-21T07:47:33Z Presence of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in local beef and Indian buffalo meat 2018-10 Kwan, Sin Yen Shiga-like toxin producing Escherichia coli bacteria are well known to be the pathogenic bacteria that cause traveller diarrhea. E. coli O157: H7 from the group of Shiga-like toxin producing E. coli cause even severe infection which can lead to fatal in human. In this study, local beef and India buffalo were selected to determine the presence of Shiga-like toxin producing E. coli and E. coli O157:H7 using Most Probable Number-Polymerase Chain Reaction (MPN-PCR) method. Among one hundred and eight samples, eight samples from local beef and India buffalo were detected a positive on E. coli O157: H7 while thirteen samples were detected positive for Shiga-like toxin producing E. coli gene. Out of 108 samples (n=108), 11 isolates of E. coli O157: H7 were successfully isolated in order to carry out the antibiotic susceptibility test. Shiga-like toxin producing E. coli isolates were found susceptible to ceftazidime (100%), moxifloxacin (83.33%), sulphamethoxazole (66.67%), ampicillin (50%), amoxycillin (50%), ciprofloxacin (50%), erythromycin (33.33%) and penicillin G (33.33%). E. coli O157: H7 isolates were susceptible toward erythromycin (100%), ceftazime (100%), ciprofloxacin (100%) and moxifloxacin (100%), sulphamethoxazole (60%), ampicillin (20%), amoxycillin (20%), and penicillin G (0%). In conclusion, both Shiga-like toxin producing E. coli and E. coli O157: H7 were detected from both local beef an India buffalo. In this research, effect of holding time and washing practice on growth pattern of E. coli O157:H7 on beef was studied. Beef samples that were washed and remain un-washed were placed in room temperature. Washed samples present a lower bacteria count. However, the longer the beef was placed under room temperature, the more the bacteria count detected on beef sample. It is recommended to keep raw meat immediately in fridge or freezer after purchase or thawing raw meat overnight in fridge (4 °C). Escherichia coli Foodborne diseases 2018-10 Thesis http://psasir.upm.edu.my/id/eprint/77782/ http://psasir.upm.edu.my/id/eprint/77782/1/FSTM%202019%207%20ir.pdf text en public masters Universiti Putra Malaysia Escherichia coli Foodborne diseases Radu, Son
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Radu, Son
topic	Escherichia coli Foodborne diseases
spellingShingle	Escherichia coli Foodborne diseases Kwan, Sin Yen Presence of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in local beef and Indian buffalo meat
description	Shiga-like toxin producing Escherichia coli bacteria are well known to be the pathogenic bacteria that cause traveller diarrhea. E. coli O157: H7 from the group of Shiga-like toxin producing E. coli cause even severe infection which can lead to fatal in human. In this study, local beef and India buffalo were selected to determine the presence of Shiga-like toxin producing E. coli and E. coli O157:H7 using Most Probable Number-Polymerase Chain Reaction (MPN-PCR) method. Among one hundred and eight samples, eight samples from local beef and India buffalo were detected a positive on E. coli O157: H7 while thirteen samples were detected positive for Shiga-like toxin producing E. coli gene. Out of 108 samples (n=108), 11 isolates of E. coli O157: H7 were successfully isolated in order to carry out the antibiotic susceptibility test. Shiga-like toxin producing E. coli isolates were found susceptible to ceftazidime (100%), moxifloxacin (83.33%), sulphamethoxazole (66.67%), ampicillin (50%), amoxycillin (50%), ciprofloxacin (50%), erythromycin (33.33%) and penicillin G (33.33%). E. coli O157: H7 isolates were susceptible toward erythromycin (100%), ceftazime (100%), ciprofloxacin (100%) and moxifloxacin (100%), sulphamethoxazole (60%), ampicillin (20%), amoxycillin (20%), and penicillin G (0%). In conclusion, both Shiga-like toxin producing E. coli and E. coli O157: H7 were detected from both local beef an India buffalo. In this research, effect of holding time and washing practice on growth pattern of E. coli O157:H7 on beef was studied. Beef samples that were washed and remain un-washed were placed in room temperature. Washed samples present a lower bacteria count. However, the longer the beef was placed under room temperature, the more the bacteria count detected on beef sample. It is recommended to keep raw meat immediately in fridge or freezer after purchase or thawing raw meat overnight in fridge (4 °C).
format	Thesis
qualification_level	Master's degree
author	Kwan, Sin Yen
author_facet	Kwan, Sin Yen
author_sort	Kwan, Sin Yen
title	Presence of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in local beef and Indian buffalo meat
title_short	Presence of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in local beef and Indian buffalo meat
title_full	Presence of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in local beef and Indian buffalo meat
title_fullStr	Presence of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in local beef and Indian buffalo meat
title_full_unstemmed	Presence of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in local beef and Indian buffalo meat
title_sort	presence of shiga-toxin producing escherichia coli and escherichia coli o157: h7 in local beef and indian buffalo meat
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/77782/1/FSTM%202019%207%20ir.pdf
_version_	1747813260319522816

Presence of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in local beef and Indian buffalo meat

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