Effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics Sprague Dawley rats

Wound healing impairment is one of the debilitating complications caused by diabetes mellitus (DM) which leads to morbidity and mortality. Previous preliminary report of our team has showed that Vicenin-2 (VCN-2) bioactive compound which possesses potent wound healing properties is identified from M...

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Main Author:	Tan, Woan Sean
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Wound healing - Animal models - Research Diabetics
Online Access:	http://psasir.upm.edu.my/id/eprint/78515/1/IB%202018%2040%20ir.pdf
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id	my-upm-ir.78515
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spelling	my-upm-ir.785152022-01-17T04:33:26Z Effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics Sprague Dawley rats 2019-03 Tan, Woan Sean Wound healing impairment is one of the debilitating complications caused by diabetes mellitus (DM) which leads to morbidity and mortality. Previous preliminary report of our team has showed that Vicenin-2 (VCN-2) bioactive compound which possesses potent wound healing properties is identified from Moringa oleifera extract (MO). The general objective of this study is to determine the wound healing efficacy of VCN-2 hydrocolloid film on diabetic rats. Firstly, the cytotoxicity and medicinal effects of VCN-2 was investigated through in vitro studies. A new sodium alginate (SA) film formulation contained VCN-2 was then optimized and developed, the various physicochemical tests were conducted on the developed VCN-2 film. Subsequently, the VCN-2 film was then tested in streptozotocin (STZ) induced male Sprague Dawley rats. The diabetes induced rat models were subjected to wound infliction and topical treatment of SA hydrocolloid film with VCN-2 in respective doses (12.5, 25 and 50 μM) for 14 days. Finally, the animals were sacrificed for wound healing assessment. Results indicated that VCN-2 hydrocolloid film has significantly reduced the production of pro-inflammatory cytokines and mediators (IL-6, IL-1β, TNF-α, NF-κB, iNOS and COX-2), while enhancing the production of healing growth factors (VEGF and TGF-β) in wound samples. During diabetic wound healing, the VCN-2 hydrocolloid film had influence inflammation phase by suppressing pro-inflammatory cytokines and proliferation phase by inducing growth factor expression. It has ameliorated prolong inflammatory process and enhanced healing through VEGF and TGF-β pathways. Taken together, VCN-2 hydrocolloid film is a promising new treatment to enhance impaired wound healing in hyperglycemic condition which prompts further investigation. Wound healing - Animal models - Research Diabetics 2019-03 Thesis http://psasir.upm.edu.my/id/eprint/78515/ http://psasir.upm.edu.my/id/eprint/78515/1/IB%202018%2040%20ir.pdf text en public doctoral Universiti Putra Malaysia Wound healing - Animal models - Research Diabetics Fakurazi, Sharida
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Fakurazi, Sharida
topic	Wound healing - Animal models - Research Diabetics
spellingShingle	Wound healing - Animal models - Research Diabetics Tan, Woan Sean Effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics Sprague Dawley rats
description	Wound healing impairment is one of the debilitating complications caused by diabetes mellitus (DM) which leads to morbidity and mortality. Previous preliminary report of our team has showed that Vicenin-2 (VCN-2) bioactive compound which possesses potent wound healing properties is identified from Moringa oleifera extract (MO). The general objective of this study is to determine the wound healing efficacy of VCN-2 hydrocolloid film on diabetic rats. Firstly, the cytotoxicity and medicinal effects of VCN-2 was investigated through in vitro studies. A new sodium alginate (SA) film formulation contained VCN-2 was then optimized and developed, the various physicochemical tests were conducted on the developed VCN-2 film. Subsequently, the VCN-2 film was then tested in streptozotocin (STZ) induced male Sprague Dawley rats. The diabetes induced rat models were subjected to wound infliction and topical treatment of SA hydrocolloid film with VCN-2 in respective doses (12.5, 25 and 50 μM) for 14 days. Finally, the animals were sacrificed for wound healing assessment. Results indicated that VCN-2 hydrocolloid film has significantly reduced the production of pro-inflammatory cytokines and mediators (IL-6, IL-1β, TNF-α, NF-κB, iNOS and COX-2), while enhancing the production of healing growth factors (VEGF and TGF-β) in wound samples. During diabetic wound healing, the VCN-2 hydrocolloid film had influence inflammation phase by suppressing pro-inflammatory cytokines and proliferation phase by inducing growth factor expression. It has ameliorated prolong inflammatory process and enhanced healing through VEGF and TGF-β pathways. Taken together, VCN-2 hydrocolloid film is a promising new treatment to enhance impaired wound healing in hyperglycemic condition which prompts further investigation.
format	Thesis
qualification_level	Doctorate
author	Tan, Woan Sean
author_facet	Tan, Woan Sean
author_sort	Tan, Woan Sean
title	Effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics Sprague Dawley rats
title_short	Effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics Sprague Dawley rats
title_full	Effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics Sprague Dawley rats
title_fullStr	Effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics Sprague Dawley rats
title_full_unstemmed	Effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics Sprague Dawley rats
title_sort	effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics sprague dawley rats
granting_institution	Universiti Putra Malaysia
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/78515/1/IB%202018%2040%20ir.pdf
_version_	1747813301982593024

Effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics Sprague Dawley rats

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