Evaluation of anthocyanins extraction from Gynura crepioides Benth. using conventional method

Gynura crepioides is one of the natural resources of flavonoid compounds, including anthocyanins (ACs). The anthocyanins, extracted from the leaves of G. crepioides, provide a lot of nutritional values to human and preferred in most of the food industries as a natural colourant. Limited studies h...

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Main Author:	Wong, Mei Lin
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Anthocyanins
Online Access:	http://psasir.upm.edu.my/id/eprint/79326/1/FK%202016%20141%20ir.pdf
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id	my-upm-ir.79326
record_format	uketd_dc
spelling	my-upm-ir.793262022-01-12T02:43:49Z Evaluation of anthocyanins extraction from Gynura crepioides Benth. using conventional method 2016-09 Wong, Mei Lin Gynura crepioides is one of the natural resources of flavonoid compounds, including anthocyanins (ACs). The anthocyanins, extracted from the leaves of G. crepioides, provide a lot of nutritional values to human and preferred in most of the food industries as a natural colourant. Limited studies have been reported on the determination of parameters for the solid-liquid extraction process and the kinetic study in ensuring its efficient performance. In this present study, freeze drying method is recommended prior to extraction to ensure high recovery of useful bioactive compounds for later use. The chemical characteristics of the G. crepioides leaves are studied using proximate analysis. Next, investigations were done to determine the effect of various extraction parameters in order to achieve high anthocyanins yield from the leaves by using the conventional solid-liquid extraction method. Those extraction parameters included the effect of solvent selection, solvent volume, methanol to aqueous HCl ratio, HCl concentration, extraction temperature, and time. The highest yield of ACs from the freeze dried leaves of G. crepioides with the use of the conventional solid-liquid extraction method was 25.89 0.50 mg/L that extracted at 50 for 15 minutes by using acidified methanol. The kinetics of extraction is also studied and the data observed corresponded well to second-order reaction. The activation energy is calculated as 18.45 kJ mol-1, which is an indication of an endothermic process. The highest initial extraction rate is observed as 16.64 mg L-1 min-1 at 333K. The extract of the leaves was subjected to DPPH scavenging activity and cytotoxicity tests. Lastly, the extract of leaves, with the presence of anthocyanins, shows a good DPPH scavenging activity and cytotoxicity. The findings suggest that extract of G. crepioides is a valuable anthocyanins sources that maybe beneficial to the industry. Anthocyanins 2016-09 Thesis http://psasir.upm.edu.my/id/eprint/79326/ http://psasir.upm.edu.my/id/eprint/79326/1/FK%202016%20141%20ir.pdf text en public masters Universiti Putra Malaysia Anthocyanins Zainal Abidin, Zurina
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Zainal Abidin, Zurina
topic	Anthocyanins
spellingShingle	Anthocyanins Wong, Mei Lin Evaluation of anthocyanins extraction from Gynura crepioides Benth. using conventional method
description	Gynura crepioides is one of the natural resources of flavonoid compounds, including anthocyanins (ACs). The anthocyanins, extracted from the leaves of G. crepioides, provide a lot of nutritional values to human and preferred in most of the food industries as a natural colourant. Limited studies have been reported on the determination of parameters for the solid-liquid extraction process and the kinetic study in ensuring its efficient performance. In this present study, freeze drying method is recommended prior to extraction to ensure high recovery of useful bioactive compounds for later use. The chemical characteristics of the G. crepioides leaves are studied using proximate analysis. Next, investigations were done to determine the effect of various extraction parameters in order to achieve high anthocyanins yield from the leaves by using the conventional solid-liquid extraction method. Those extraction parameters included the effect of solvent selection, solvent volume, methanol to aqueous HCl ratio, HCl concentration, extraction temperature, and time. The highest yield of ACs from the freeze dried leaves of G. crepioides with the use of the conventional solid-liquid extraction method was 25.89 0.50 mg/L that extracted at 50 for 15 minutes by using acidified methanol. The kinetics of extraction is also studied and the data observed corresponded well to second-order reaction. The activation energy is calculated as 18.45 kJ mol-1, which is an indication of an endothermic process. The highest initial extraction rate is observed as 16.64 mg L-1 min-1 at 333K. The extract of the leaves was subjected to DPPH scavenging activity and cytotoxicity tests. Lastly, the extract of leaves, with the presence of anthocyanins, shows a good DPPH scavenging activity and cytotoxicity. The findings suggest that extract of G. crepioides is a valuable anthocyanins sources that maybe beneficial to the industry.
format	Thesis
qualification_level	Master's degree
author	Wong, Mei Lin
author_facet	Wong, Mei Lin
author_sort	Wong, Mei Lin
title	Evaluation of anthocyanins extraction from Gynura crepioides Benth. using conventional method
title_short	Evaluation of anthocyanins extraction from Gynura crepioides Benth. using conventional method
title_full	Evaluation of anthocyanins extraction from Gynura crepioides Benth. using conventional method
title_fullStr	Evaluation of anthocyanins extraction from Gynura crepioides Benth. using conventional method
title_full_unstemmed	Evaluation of anthocyanins extraction from Gynura crepioides Benth. using conventional method
title_sort	evaluation of anthocyanins extraction from gynura crepioides benth. using conventional method
granting_institution	Universiti Putra Malaysia
publishDate	2016
url	http://psasir.upm.edu.my/id/eprint/79326/1/FK%202016%20141%20ir.pdf
_version_	1747813316066017280

Evaluation of anthocyanins extraction from Gynura crepioides Benth. using conventional method

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