Genetic Diversity Analysis of Sweet Potato (Ipomoea Batatas L.) Germplasm from Malaysia and Indonesia Using RAPD Markers
Genetic variation among sweetpotato accession (Ipomoea batatas l.) from Malaysia and Indonesia has not been extensively examined with molecular markers. The objectives of this study are to use random amplified polymorphic DNA (RAPD) marker to determine the degree of polymorphism in the sweetpota...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2001
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/7953/1/IB_2001_2_.pdf |
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| Summary: | Genetic variation among sweetpotato accession (Ipomoea batatas l.)
from Malaysia and Indonesia has not been extensively examined with
molecular markers. The objectives of this study are to use random
amplified polymorphic DNA (RAPD) marker to determine the degree of
polymorphism in the sweetpotato germ plasm and to study the genetiC
diversity and relationships among sweetpotato accessions from two
different regions, Malaysia and Indonesia.
A total of 92 accessions of sweet potato germplasm from the two
countries maintained at Universiti Putra Malaysia were characterized
using five RAPD· markers. Thirty-nine accessions were collected from
seven different states of Malaysia and 53 accessions came from two
different sub-regions of Indonesia (Irian Jaya and Java).
The results of this study indicated that the levels of polymorphism
among all sweetpotato were extremely high. From five random primers used (OPB 07, ope 1 0, OPO 0 1, OPO 06 and OPG 1 4), 1 94 fragments
were amplified , of which 1 92 (98. 97%) were polymorphic. Only two
fragments were monomorphic. The fragment size ranged from 1 1 7bp -
3 240bp.
An NTSYS-pc computer program was further employed for data analysis
using Jaccard's coefficient of similarity as a base for dendrogram
construction via the U PGMA method. The Jaccard's similarity values
ranged from 0.08 to 0.6 9 showing high levels of genetic variability
among sweetpotato accessions. The cluster analysis separated
Malaysian and Indonesian accessions i nto a d ifferent group with a
number of additional clusters. Some of the Malaysian and Indonesian
accessions were clustered based on their geographic source. The
analysis i ndicates that very large genetic variation exists among
sweetpotato accessions used in this study and the sweetpotato
collection is a valuable as a genetic resource. This could be done by
selecting cultivars from different groups delineated by cluster analysis for
hybridization programs.
Genetic diversity analysis within the sweetpotato germplasm collection
had provided useful i nformation for managing thi s collection. RAPO
appears to be useful for discerning variation within crop germplasm and
to assess the genetic relationships among sweetpotato germ plasm from
Malaysia and Indonesia. |
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