Effects of Lactobacillus Strains as a Probiotic and a Hypolipidaemic Agent for Chickens

In recent years, there has been considerable interest in the beneficial effects of probiotics (direct-fed microbials, which include Lactobacillus) to modulate the lipid metabolism. However, the mechanism(s) involved remains unclear. A series of experiments was carried out to investigate the abili...

Full description

Saved in:

Bibliographic Details
Main Author:	Ramasamy, Kalavathy
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Lactobacillus - Molecular aspects Anticholesteremic agents Cholesterol - Metabolism
Online Access:	http://psasir.upm.edu.my/id/eprint/7963/1/IB_2003_2.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.7963
record_format	uketd_dc
spelling	my-upm-ir.79632023-11-29T01:29:55Z Effects of Lactobacillus Strains as a Probiotic and a Hypolipidaemic Agent for Chickens 2003-08 Ramasamy, Kalavathy In recent years, there has been considerable interest in the beneficial effects of probiotics (direct-fed microbials, which include Lactobacillus) to modulate the lipid metabolism. However, the mechanism(s) involved remains unclear. A series of experiments was carried out to investigate the ability of 1 2 Lactobacillus strains to deconj ugate bile salts and to remove cholesterol in vitro, and to assess their potential as a pro biotic and as a hypolipidaemic agent for broilers and laying hens. Bile salt hydrolase (BSH) activity (resulting in bile salt deconjugation) of intestinal bacteria is closely linked to the lowering of cholesterol. The results of the in vitro studies showed that all the 1 2 Lactobacillus strains could deconjugate sodium glychocholate (GCA) and sodium taurocholate (TCA) bile salts, and all the strains, except L. fermentum I 24, had a higher affinity for GCA. However, only eight strains could deconjugate sodium taurodeoxycholate (TDCA). This indicates that the BSH of the Lactobacillus strains is substrate specific. The 1 2 Lactobacillus strains showed significant differences in their ability to reduce cholesterol from the growth medium (27 to 85 %) with or without bile salt, indicating that bile salt is not a prerequisite for the removal of cholesterol. Lactobacillus acidophilus I 1 6, L. crispatus I 1 2, L. brevis C 1 7 and I 2 1 1 , and L. Jermentum I 24 and I 25 removed cholesterol from the growth medium mainly through assimilation of cholesterol into the cells. On the other hand, L. brevis C 1 , C 1 0, I 23 and I 2 1 8, and L. Jermentum C 1 6 removed cholesterol through both assimilation and co-precipitation of deconjugated bile salt with cholesterol at low pH. The Lactobacillus strains assimilated more esterified than non-esterified cholesterol and the assimilated cholesterol was tightly bound to the cells. Cells grown in the presence of cholesterol were more resistant to lysis by sonication than when grown in its absence, suggesting a possible alteration of the cell wall or membrane by the assimilated cholesterol. Cholesterol removal by the Lactobacillus strains was also affected by Tween 80. The feeding trials showed that the supplementation of a mixture of the 1 2 Lactobacillus cultures (LC), as a probiotic for broilers, significantly improved growth equivalent to that provided by the antibiotic, oxytetracycline, but the feed conversion ratio was better in LC-fed broilers. The supplementation of LC also significantly lowered the total cholesterol, low density lipoprotein cholesterol and triglycerides of the serum; the cholesterol of the carcass and liver; abdominal fat deposition; and fat contents of the liver, muscle and carcass of broilers; but there was little effect on the fatty acid compositions of the liver, muscle and carcass. In laying hens, the supplementation of LC improved the feed efficiency and hen-day egg production during the early stage of the laying cycle, and increased egg weight and influenced a shift from small and medium to large and extra large eggs throughout the laying cycle. However, LC had very little effect on improving the fatty acid composition, and the cholesterol and total fat contents of eggs. Lactobacillus - Molecular aspects Anticholesteremic agents Cholesterol - Metabolism 2003-08 Thesis http://psasir.upm.edu.my/id/eprint/7963/ http://psasir.upm.edu.my/id/eprint/7963/1/IB_2003_2.pdf text en public doctoral Universiti Putra Malaysia Lactobacillus - Molecular aspects Anticholesteremic agents Cholesterol - Metabolism Institute of Bioscience Ho, Yin Wan English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Ho, Yin Wan
topic	Lactobacillus - Molecular aspects Anticholesteremic agents Cholesterol - Metabolism
spellingShingle	Lactobacillus - Molecular aspects Anticholesteremic agents Cholesterol - Metabolism Ramasamy, Kalavathy Effects of Lactobacillus Strains as a Probiotic and a Hypolipidaemic Agent for Chickens
description	In recent years, there has been considerable interest in the beneficial effects of probiotics (direct-fed microbials, which include Lactobacillus) to modulate the lipid metabolism. However, the mechanism(s) involved remains unclear. A series of experiments was carried out to investigate the ability of 1 2 Lactobacillus strains to deconj ugate bile salts and to remove cholesterol in vitro, and to assess their potential as a pro biotic and as a hypolipidaemic agent for broilers and laying hens. Bile salt hydrolase (BSH) activity (resulting in bile salt deconjugation) of intestinal bacteria is closely linked to the lowering of cholesterol. The results of the in vitro studies showed that all the 1 2 Lactobacillus strains could deconjugate sodium glychocholate (GCA) and sodium taurocholate (TCA) bile salts, and all the strains, except L. fermentum I 24, had a higher affinity for GCA. However, only eight strains could deconjugate sodium taurodeoxycholate (TDCA). This indicates that the BSH of the Lactobacillus strains is substrate specific. The 1 2 Lactobacillus strains showed significant differences in their ability to reduce cholesterol from the growth medium (27 to 85 %) with or without bile salt, indicating that bile salt is not a prerequisite for the removal of cholesterol. Lactobacillus acidophilus I 1 6, L. crispatus I 1 2, L. brevis C 1 7 and I 2 1 1 , and L. Jermentum I 24 and I 25 removed cholesterol from the growth medium mainly through assimilation of cholesterol into the cells. On the other hand, L. brevis C 1 , C 1 0, I 23 and I 2 1 8, and L. Jermentum C 1 6 removed cholesterol through both assimilation and co-precipitation of deconjugated bile salt with cholesterol at low pH. The Lactobacillus strains assimilated more esterified than non-esterified cholesterol and the assimilated cholesterol was tightly bound to the cells. Cells grown in the presence of cholesterol were more resistant to lysis by sonication than when grown in its absence, suggesting a possible alteration of the cell wall or membrane by the assimilated cholesterol. Cholesterol removal by the Lactobacillus strains was also affected by Tween 80. The feeding trials showed that the supplementation of a mixture of the 1 2 Lactobacillus cultures (LC), as a probiotic for broilers, significantly improved growth equivalent to that provided by the antibiotic, oxytetracycline, but the feed conversion ratio was better in LC-fed broilers. The supplementation of LC also significantly lowered the total cholesterol, low density lipoprotein cholesterol and triglycerides of the serum; the cholesterol of the carcass and liver; abdominal fat deposition; and fat contents of the liver, muscle and carcass of broilers; but there was little effect on the fatty acid compositions of the liver, muscle and carcass. In laying hens, the supplementation of LC improved the feed efficiency and hen-day egg production during the early stage of the laying cycle, and increased egg weight and influenced a shift from small and medium to large and extra large eggs throughout the laying cycle. However, LC had very little effect on improving the fatty acid composition, and the cholesterol and total fat contents of eggs.
format	Thesis
qualification_level	Doctorate
author	Ramasamy, Kalavathy
author_facet	Ramasamy, Kalavathy
author_sort	Ramasamy, Kalavathy
title	Effects of Lactobacillus Strains as a Probiotic and a Hypolipidaemic Agent for Chickens
title_short	Effects of Lactobacillus Strains as a Probiotic and a Hypolipidaemic Agent for Chickens
title_full	Effects of Lactobacillus Strains as a Probiotic and a Hypolipidaemic Agent for Chickens
title_fullStr	Effects of Lactobacillus Strains as a Probiotic and a Hypolipidaemic Agent for Chickens
title_full_unstemmed	Effects of Lactobacillus Strains as a Probiotic and a Hypolipidaemic Agent for Chickens
title_sort	effects of lactobacillus strains as a probiotic and a hypolipidaemic agent for chickens
granting_institution	Universiti Putra Malaysia
granting_department	Institute of Bioscience
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/7963/1/IB_2003_2.pdf
_version_	1794018704466378752

Effects of Lactobacillus Strains as a Probiotic and a Hypolipidaemic Agent for Chickens

Similar Items