Morphometric and performance of Pangasianodon hypophthalmus (Sauvage, 1983) (♀) × Pangasius nasutus (Bleeker, 1976) (♂) hybrid

Morphometric and performance of Pangasianodon hypophthalmus (Sauvage, 1983) (♀) × Pangasius nasutus (Bleeker, 1976) (♂) hybrid

The present study was carried out to assess the morphometric variation and performance of crossbreed of Pangasianodon hypophthalmus (♀) and Pangasius nasutus (♂), (hybrid PH×PN) and its parental species; female, PH (P. hypophthalmus ♀ × P. hypophthalmus ♂); male, PN (P. nasutus ♀ × P. nasutus ♂). Th...

全面介绍

Saved in:
书目详细资料
主要作者: Mohamed Yusoff, Siti Fairus
格式: Thesis
语言:English
出版: 2019
主题:
Pangasianodon hypophthalmus
Morphophonemics
在线阅读:http://psasir.upm.edu.my/id/eprint/82926/1/FP%202019%2024%20ir.pdf
标签: 添加标签
没有标签, 成为第一个标记此记录!
id my-upm-ir.82926
record_format uketd_dc
spelling my-upm-ir.829262022-01-11T06:54:02Z Morphometric and performance of Pangasianodon hypophthalmus (Sauvage, 1983) (♀) × Pangasius nasutus (Bleeker, 1976) (♂) hybrid 2019-01 Mohamed Yusoff, Siti Fairus The present study was carried out to assess the morphometric variation and performance of crossbreed of Pangasianodon hypophthalmus (♀) and Pangasius nasutus (♂), (hybrid PH×PN) and its parental species; female, PH (P. hypophthalmus ♀ × P. hypophthalmus ♂); male, PN (P. nasutus ♀ × P. nasutus ♂). The first objective was employed to evaluate the feasibility (breeding performance, growth, survival) and biochemical composition (proximate, fatty acid, and amino acid profiles) of hybrid PH×PN and its parental species. Breeding, growth and survival performance of hybrid PH×PN based on fertilization, hatching, deformity, total length (TL), body weight (BW) and survival resulted in 73.50±1.34%, 65.43±2.24%, 9.93±1.70%, 32.12±5.56 mm, 107.71±9.91 mg, 62.5±6.50%, respectively, which were higher than in P. nasutus, 58.33±1.37%, 13.80±1.05%, 6.98±0.43%, 30.30±1.70 mm, 129.48±15.88 mg, 15.00±15.19%, respectively. Hybrid PH×PN showed higher protein content (25.09±0.22%) than both parental species (PH; 24.86±0.34%; PN: 23.55±0.24%) and higher lipid content (4.34±0.07%) than P. hypophthalmus (3.48±0.05%). Fatty acid and amino acid profiles varied between species, demonstrating high polyunsaturated fatty acids (PUFA) (26.32±1.66%) and total essential amino acids (EAA) (45.56±0.76%) in hybrid PH×PN than its parental species. The second objective examined the morphological variation of hybrid PH×PN and its parental species. Results revealed a distinct vomerin and palatal teeth of hybrid PH×PN. Ten out of the thirty morphometric characters measured, which include prepelvic length, caudal peduncle length, dorsal fin length, pectoral fin length, adipose fin height, anal fin length, adipose fin length, interorbital length, distant snout to isthmus, and predorsal length are the strongest predictors that contributed meaningfully to the species discrimination. The third objective was carried out to characterize the genetic variation of hybrid PH×PN based on molecular marker using mitochondrial DNA (mtDNA) of Cytochrome Oxidase (I) (COI) gene to infer the maternal lineage of hybrid PH×PN and microsatellite marker applied to elucidate the genetic variation. Finding of COI gene revealed hybrid PH×PN shared the same haplotype (HPH1) as P.hypophthalmus which proved the direction of maternal mating of hybrid PH×PN. Microsatellites marker showed genetic variability of hybrid PH×PN as observed over the loci. The fourth objective evaluated the resistance of hybrid PH×PN and its parental species towards bacteria, Aeromonas hydrophila. Results revealed the 96 h-LD50 value of hybrid PH×PN (×105.16 cfu mL-1) was higher than P. nasutus (×103.51 cfu mL-1) but lower than P. hypophthalmus (×106.67 cfu mL-1). Immunological response of the respiratory burst and lysozyme activities, however, significantly higher in hybrid PH×PN prior to challenge but decreased subsequently at 46 and 96 h post challenged. In conclusion, findings of this study revealed morphometric variation is beneficial in identifying and discriminating hybrid PH×PN from its parental species prior to using molecular markers. Genetic characterization using mitochondrial COI I inferred the maternal lineage of hybrid PH×PN. There is a promising performance of important traits in regard to growth, disease resistance, flesh quality particularly on the protein content than P. nasutus, revealing its potential as species for aquaculture. Pangasianodon hypophthalmus Morphophonemics 2019-01 Thesis http://psasir.upm.edu.my/id/eprint/82926/ http://psasir.upm.edu.my/id/eprint/82926/1/FP%202019%2024%20ir.pdf text en public doctoral Universiti Putra Malaysia Pangasianodon hypophthalmus Morphophonemics Christianus, Annie
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
advisor Christianus, Annie
topic Pangasianodon hypophthalmus
Morphophonemics
spellingShingle Pangasianodon hypophthalmus
Morphophonemics

Mohamed Yusoff, Siti Fairus
Morphometric and performance of Pangasianodon hypophthalmus (Sauvage, 1983) (♀) × Pangasius nasutus (Bleeker, 1976) (♂) hybrid
description The present study was carried out to assess the morphometric variation and performance of crossbreed of Pangasianodon hypophthalmus (♀) and Pangasius nasutus (♂), (hybrid PH×PN) and its parental species; female, PH (P. hypophthalmus ♀ × P. hypophthalmus ♂); male, PN (P. nasutus ♀ × P. nasutus ♂). The first objective was employed to evaluate the feasibility (breeding performance, growth, survival) and biochemical composition (proximate, fatty acid, and amino acid profiles) of hybrid PH×PN and its parental species. Breeding, growth and survival performance of hybrid PH×PN based on fertilization, hatching, deformity, total length (TL), body weight (BW) and survival resulted in 73.50±1.34%, 65.43±2.24%, 9.93±1.70%, 32.12±5.56 mm, 107.71±9.91 mg, 62.5±6.50%, respectively, which were higher than in P. nasutus, 58.33±1.37%, 13.80±1.05%, 6.98±0.43%, 30.30±1.70 mm, 129.48±15.88 mg, 15.00±15.19%, respectively. Hybrid PH×PN showed higher protein content (25.09±0.22%) than both parental species (PH; 24.86±0.34%; PN: 23.55±0.24%) and higher lipid content (4.34±0.07%) than P. hypophthalmus (3.48±0.05%). Fatty acid and amino acid profiles varied between species, demonstrating high polyunsaturated fatty acids (PUFA) (26.32±1.66%) and total essential amino acids (EAA) (45.56±0.76%) in hybrid PH×PN than its parental species. The second objective examined the morphological variation of hybrid PH×PN and its parental species. Results revealed a distinct vomerin and palatal teeth of hybrid PH×PN. Ten out of the thirty morphometric characters measured, which include prepelvic length, caudal peduncle length, dorsal fin length, pectoral fin length, adipose fin height, anal fin length, adipose fin length, interorbital length, distant snout to isthmus, and predorsal length are the strongest predictors that contributed meaningfully to the species discrimination. The third objective was carried out to characterize the genetic variation of hybrid PH×PN based on molecular marker using mitochondrial DNA (mtDNA) of Cytochrome Oxidase (I) (COI) gene to infer the maternal lineage of hybrid PH×PN and microsatellite marker applied to elucidate the genetic variation. Finding of COI gene revealed hybrid PH×PN shared the same haplotype (HPH1) as P.hypophthalmus which proved the direction of maternal mating of hybrid PH×PN. Microsatellites marker showed genetic variability of hybrid PH×PN as observed over the loci. The fourth objective evaluated the resistance of hybrid PH×PN and its parental species towards bacteria, Aeromonas hydrophila. Results revealed the 96 h-LD50 value of hybrid PH×PN (×105.16 cfu mL-1) was higher than P. nasutus (×103.51 cfu mL-1) but lower than P. hypophthalmus (×106.67 cfu mL-1). Immunological response of the respiratory burst and lysozyme activities, however, significantly higher in hybrid PH×PN prior to challenge but decreased subsequently at 46 and 96 h post challenged. In conclusion, findings of this study revealed morphometric variation is beneficial in identifying and discriminating hybrid PH×PN from its parental species prior to using molecular markers. Genetic characterization using mitochondrial COI I inferred the maternal lineage of hybrid PH×PN. There is a promising performance of important traits in regard to growth, disease resistance, flesh quality particularly on the protein content than P. nasutus, revealing its potential as species for aquaculture.
format Thesis
qualification_level Doctorate
author Mohamed Yusoff, Siti Fairus
author_facet Mohamed Yusoff, Siti Fairus
author_sort Mohamed Yusoff, Siti Fairus
title Morphometric and performance of Pangasianodon hypophthalmus (Sauvage, 1983) (♀) × Pangasius nasutus (Bleeker, 1976) (♂) hybrid
title_short Morphometric and performance of Pangasianodon hypophthalmus (Sauvage, 1983) (♀) × Pangasius nasutus (Bleeker, 1976) (♂) hybrid
title_full Morphometric and performance of Pangasianodon hypophthalmus (Sauvage, 1983) (♀) × Pangasius nasutus (Bleeker, 1976) (♂) hybrid
title_fullStr Morphometric and performance of Pangasianodon hypophthalmus (Sauvage, 1983) (♀) × Pangasius nasutus (Bleeker, 1976) (♂) hybrid
title_full_unstemmed Morphometric and performance of Pangasianodon hypophthalmus (Sauvage, 1983) (♀) × Pangasius nasutus (Bleeker, 1976) (♂) hybrid
title_sort morphometric and performance of pangasianodon hypophthalmus (sauvage, 1983) (♀) × pangasius nasutus (bleeker, 1976) (♂) hybrid
granting_institution Universiti Putra Malaysia
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/82926/1/FP%202019%2024%20ir.pdf
_version_ 1747813330061361152

相似书籍