Potential of Malaysian seaweed extracts in inhibiting Ganoderma boninense and identification of their antifungal compounds

Basal stem rot (BSR) disease is the most devastating disease in oil palm (Elaies guineensis) caused by a fungal pathogen, Ganoderma boninense. Various control measures have been tested but to date the utilization of seaweeds as a source of control agent has not been explored. This study investigated...

Full description

Saved in:

Bibliographic Details
Main Author:	Abdul Aziz, Syamimi Diyana
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Ganoderma Antifungal agents Ganoderma diseases of plants
Online Access:	http://psasir.upm.edu.my/id/eprint/83019/1/FBSB%202018%2057%20ir.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.83019
record_format	uketd_dc
spelling	my-upm-ir.830192022-01-11T03:23:05Z Potential of Malaysian seaweed extracts in inhibiting Ganoderma boninense and identification of their antifungal compounds 2018-12 Abdul Aziz, Syamimi Diyana Basal stem rot (BSR) disease is the most devastating disease in oil palm (Elaies guineensis) caused by a fungal pathogen, Ganoderma boninense. Various control measures have been tested but to date the utilization of seaweeds as a source of control agent has not been explored. This study investigated the antifungal potential of Malaysian seaweed extracts against G. boninense and identifying the potential antifungal compounds. Polar and non- polar compounds were extracted from cfSargassum oligocystum, Caulerpa racemosa, Caulerpa racemosa var. lamourouxii and cfHalimeda macrophysa using water, methanol, chloroform, dichloromethane and hexane. Methanolic extracts from all species displayed the highest yield with an average of 15.25 % compared to dry weight. At 0.25 mg/mL, C. racemosa var. lamourouxii dichloromethane extract recorded the highest growth reduction against G. boninense with 46.82 % followed by C. racemosa var. lamourouxii hexane extract with 36.43 % and cfH. macrophysa dichloromethane extract with 33.49 %. Statistical analysis from Kruskal-Wallis H test showed significant difference (H=77.23, p=.00) in growth reduction percentage between the different solvent. Dominant compounds detected via Gas Chromatography-Mass Spectrometry in extracts with antifungal potential were l-(+)-ascorbic acid 2,6-dihexadecanoate, phytol, tetradecanoic acid, isobutyl methylphosphonofluoridate and benzenamine,2-[2-(4-pyridinyl) ethyl]-. Further antifungal testing using standard phytol showed growth inhibition of G. boninense with 21 % inhibition and the amount of phytol in dichloromethane extracts were quantified via GC-MS. Dichloromethane extract of C. racemosa var. lamourouxii, cfH. macrophysa, C. racemosa and cfS. oligocystum contain 474, 117, 106 and 19 mg/L of phytol, respectively which were consistent with exhibited antifungal activities. These findings suggested that Malaysian seaweeds have potential as a source of natural and potent antifungal compounds for utilization in controlling the oil palm disease in Malaysia. Ganoderma Antifungal agents Ganoderma diseases of plants 2018-12 Thesis http://psasir.upm.edu.my/id/eprint/83019/ http://psasir.upm.edu.my/id/eprint/83019/1/FBSB%202018%2057%20ir.pdf text en public masters Universiti Putra Malaysia Ganoderma Antifungal agents Ganoderma diseases of plants Balia Yusof, Zetty Norhana
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Balia Yusof, Zetty Norhana
topic	Ganoderma Antifungal agents Ganoderma diseases of plants
spellingShingle	Ganoderma Antifungal agents Ganoderma diseases of plants Abdul Aziz, Syamimi Diyana Potential of Malaysian seaweed extracts in inhibiting Ganoderma boninense and identification of their antifungal compounds
description	Basal stem rot (BSR) disease is the most devastating disease in oil palm (Elaies guineensis) caused by a fungal pathogen, Ganoderma boninense. Various control measures have been tested but to date the utilization of seaweeds as a source of control agent has not been explored. This study investigated the antifungal potential of Malaysian seaweed extracts against G. boninense and identifying the potential antifungal compounds. Polar and non- polar compounds were extracted from cfSargassum oligocystum, Caulerpa racemosa, Caulerpa racemosa var. lamourouxii and cfHalimeda macrophysa using water, methanol, chloroform, dichloromethane and hexane. Methanolic extracts from all species displayed the highest yield with an average of 15.25 % compared to dry weight. At 0.25 mg/mL, C. racemosa var. lamourouxii dichloromethane extract recorded the highest growth reduction against G. boninense with 46.82 % followed by C. racemosa var. lamourouxii hexane extract with 36.43 % and cfH. macrophysa dichloromethane extract with 33.49 %. Statistical analysis from Kruskal-Wallis H test showed significant difference (H=77.23, p=.00) in growth reduction percentage between the different solvent. Dominant compounds detected via Gas Chromatography-Mass Spectrometry in extracts with antifungal potential were l-(+)-ascorbic acid 2,6-dihexadecanoate, phytol, tetradecanoic acid, isobutyl methylphosphonofluoridate and benzenamine,2-[2-(4-pyridinyl) ethyl]-. Further antifungal testing using standard phytol showed growth inhibition of G. boninense with 21 % inhibition and the amount of phytol in dichloromethane extracts were quantified via GC-MS. Dichloromethane extract of C. racemosa var. lamourouxii, cfH. macrophysa, C. racemosa and cfS. oligocystum contain 474, 117, 106 and 19 mg/L of phytol, respectively which were consistent with exhibited antifungal activities. These findings suggested that Malaysian seaweeds have potential as a source of natural and potent antifungal compounds for utilization in controlling the oil palm disease in Malaysia.
format	Thesis
qualification_level	Master's degree
author	Abdul Aziz, Syamimi Diyana
author_facet	Abdul Aziz, Syamimi Diyana
author_sort	Abdul Aziz, Syamimi Diyana
title	Potential of Malaysian seaweed extracts in inhibiting Ganoderma boninense and identification of their antifungal compounds
title_short	Potential of Malaysian seaweed extracts in inhibiting Ganoderma boninense and identification of their antifungal compounds
title_full	Potential of Malaysian seaweed extracts in inhibiting Ganoderma boninense and identification of their antifungal compounds
title_fullStr	Potential of Malaysian seaweed extracts in inhibiting Ganoderma boninense and identification of their antifungal compounds
title_full_unstemmed	Potential of Malaysian seaweed extracts in inhibiting Ganoderma boninense and identification of their antifungal compounds
title_sort	potential of malaysian seaweed extracts in inhibiting ganoderma boninense and identification of their antifungal compounds
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/83019/1/FBSB%202018%2057%20ir.pdf
_version_	1747813338171047936

Potential of Malaysian seaweed extracts in inhibiting Ganoderma boninense and identification of their antifungal compounds

Similar Items