Prebiotic properties evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model

The red seaweed Kappaphycus alvarezii is rich in carrageenan polysaccharides that are used in hydrocolloids industry for gelling and thickening agents in pharmaceutical, biotechnology and food application. It has been claimed that the carrageenan is not digestible by human. Present study builds on...

Full description

Saved in:

Bibliographic Details
Main Author:	Abang Bajury, Dayang Marshitah
Format:	Thesis
Language:	English English
Published:	2018
Subjects:	Marine algae - Therapeutic use Marine algae
Online Access:	http://psasir.upm.edu.my/id/eprint/83272/1/t%20FSPM%202018%201%20%281800001044%29.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.83272
record_format	uketd_dc
spelling	my-upm-ir.832722022-01-07T03:05:26Z Prebiotic properties evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model 2018-02 Abang Bajury, Dayang Marshitah The red seaweed Kappaphycus alvarezii is rich in carrageenan polysaccharides that are used in hydrocolloids industry for gelling and thickening agents in pharmaceutical, biotechnology and food application. It has been claimed that the carrageenan is not digestible by human. Present study builds on that knowledge to test the potential of red seaweed as a prebiotic through in vitro digestion and fermentation. The objectives of this study are to determine the digestibility of K. alvarezii through in vitro gastric and duodenal digestion, to analyze the metabolite short chain fatty acids (SCFAs) generated by the fermentation of seaweeds by using high performance liquid chromatography (HPLC) and to evaluate the fermentation selectivity of seaweeds by the human feces microbiota by using fluorescent in situ hybridization (FISH) technique. The digestibility of red seaweeds (Kappaphycus alvarezii) cultivated from Sabah (RSS) and Langkawi (RSL) was determined using in vitro gastric and duodenal digestion model that mimicking human gastrointestinal tract condition. The resulting fractions of seaweeds that resisted gastric and duodenal digestion were used as substrates for fermentation in pHcontrolled batch culture system inoculated with human feces to mimic human distal colon condition. Inulin was used as positive control and samples were taken at 0, 6, 12 and 24 h of fermentation for bacterial enumeration and SCFAs analysis. Red seaweed from Sabah (RSS) showed no significant difference with inulin for its non-digestibility while red seaweed from Langkawi (RSL) showed the highest digestibility (4.55%) by in vitro gastric and duodenal digestion. Both RSS and RSL showed significant increase of Bifidobacterium sp.; from log10 7.96 at 0 h to log10 8.72 at 24 h, and from log10 7.96 at 0 h to log10 8.60 at 24 h respectively, but at 24 h both seaweeds showed no significant difference when compared to the Bifidobacterium sp. count at the end of the inulin fermentation. The growth for Clostridium sp. in RSS (from log10 at 0 h 6.78 to log10 7.27 at 24 h) and RSL (from log10 6.78 at 0 h to log10 7.24 at 24 h) also showed no significant difference in comparison to inulin (from log10 6.78 at 0 h to log10 7.26 at 24 h). Inulin and RSS showed significant increase in total SCFA production after 24 h of fermentation, particularly in acetate and propionate. While for RSL, the total SCFA showed significant increased at 6 h, however, the total SCFA showed no significant difference after 12 and 24 h. Overall, this data suggested that RSS have potential as a prebiotic similar to the positive control, inulin compared to RSL. This is because RSS shows more bifidogenic factor and high total SCFAs production compare to RSL. Marine algae - Therapeutic use Marine algae 2018-02 Thesis http://psasir.upm.edu.my/id/eprint/83272/ http://psasir.upm.edu.my/id/eprint/83272/1/t%20FSPM%202018%201%20%281800001044%29.pdf text en public masters Universiti Putra Malaysia Marine algae - Therapeutic use Marine algae Sarbini, Shahrul Razid English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Sarbini, Shahrul Razid
topic	Marine algae - Therapeutic use Marine algae
spellingShingle	Marine algae - Therapeutic use Marine algae Abang Bajury, Dayang Marshitah Prebiotic properties evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model
description	The red seaweed Kappaphycus alvarezii is rich in carrageenan polysaccharides that are used in hydrocolloids industry for gelling and thickening agents in pharmaceutical, biotechnology and food application. It has been claimed that the carrageenan is not digestible by human. Present study builds on that knowledge to test the potential of red seaweed as a prebiotic through in vitro digestion and fermentation. The objectives of this study are to determine the digestibility of K. alvarezii through in vitro gastric and duodenal digestion, to analyze the metabolite short chain fatty acids (SCFAs) generated by the fermentation of seaweeds by using high performance liquid chromatography (HPLC) and to evaluate the fermentation selectivity of seaweeds by the human feces microbiota by using fluorescent in situ hybridization (FISH) technique. The digestibility of red seaweeds (Kappaphycus alvarezii) cultivated from Sabah (RSS) and Langkawi (RSL) was determined using in vitro gastric and duodenal digestion model that mimicking human gastrointestinal tract condition. The resulting fractions of seaweeds that resisted gastric and duodenal digestion were used as substrates for fermentation in pHcontrolled batch culture system inoculated with human feces to mimic human distal colon condition. Inulin was used as positive control and samples were taken at 0, 6, 12 and 24 h of fermentation for bacterial enumeration and SCFAs analysis. Red seaweed from Sabah (RSS) showed no significant difference with inulin for its non-digestibility while red seaweed from Langkawi (RSL) showed the highest digestibility (4.55%) by in vitro gastric and duodenal digestion. Both RSS and RSL showed significant increase of Bifidobacterium sp.; from log10 7.96 at 0 h to log10 8.72 at 24 h, and from log10 7.96 at 0 h to log10 8.60 at 24 h respectively, but at 24 h both seaweeds showed no significant difference when compared to the Bifidobacterium sp. count at the end of the inulin fermentation. The growth for Clostridium sp. in RSS (from log10 at 0 h 6.78 to log10 7.27 at 24 h) and RSL (from log10 6.78 at 0 h to log10 7.24 at 24 h) also showed no significant difference in comparison to inulin (from log10 6.78 at 0 h to log10 7.26 at 24 h). Inulin and RSS showed significant increase in total SCFA production after 24 h of fermentation, particularly in acetate and propionate. While for RSL, the total SCFA showed significant increased at 6 h, however, the total SCFA showed no significant difference after 12 and 24 h. Overall, this data suggested that RSS have potential as a prebiotic similar to the positive control, inulin compared to RSL. This is because RSS shows more bifidogenic factor and high total SCFAs production compare to RSL.
format	Thesis
qualification_level	Master's degree
author	Abang Bajury, Dayang Marshitah
author_facet	Abang Bajury, Dayang Marshitah
author_sort	Abang Bajury, Dayang Marshitah
title	Prebiotic properties evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model
title_short	Prebiotic properties evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model
title_full	Prebiotic properties evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model
title_fullStr	Prebiotic properties evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model
title_full_unstemmed	Prebiotic properties evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model
title_sort	prebiotic properties evaluation of red seaweed (kappaphycus alvarezii) using in vitro colon model
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/83272/1/t%20FSPM%202018%201%20%281800001044%29.pdf
_version_	1747813370090749952

Prebiotic properties evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model

Similar Items