Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R

Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R

Cathepsin D from the muscle of bighead carp (Aristichthys no bilis, R.)was extracted ,purified and partially characterized . The extract ion and purification o f the enzyme was achieved byautolysis of the muscle,acetone precipitation, gelfiltrat ion on Sephadex G100-120 and on ionexchange carbox...

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Main Author: Bakar, Jamilah
Format: Thesis
Language:English
English
Published: 1993
Subjects:
Bighead carp
Animal extracts
Peptides
Online Access:http://psasir.upm.edu.my/id/eprint/8357/1/FSMB_1993_1_A.pdf
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spelling my-upm-ir.83572012-10-24T07:39:55Z Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R 1993 Bakar, Jamilah Cathepsin D from the muscle of bighead carp (Aristichthys no bilis, R.)was extracted ,purified and partially characterized . The extract ion and purification o f the enzyme was achieved byautolysis of the muscle,acetone precipitation, gelfiltrat ion on Sephadex G100-120 and on ionexchange carboxymethyl cellulose (CMC)column chromatography . It had a molecular weight(m.w)of 37,500-38,000 dalton (D) with a pH optimum at 3.2 and temperature optimum of 50°C. Myofibril was also optimally digested at pH3.2. The purified enzyme had a single major peptideband on sodiumdodecyl sulfate polyacrylamide gelelectrophoresis SDS-PAGE )and was completely inhibited by pepstatin . Bighead carp Animal extracts Peptides 1993 Thesis http://psasir.upm.edu.my/id/eprint/8357/ http://psasir.upm.edu.my/id/eprint/8357/1/FSMB_1993_1_A.pdf application/pdf en public phd doctoral Universiti Pertanian Malaysia Bighead carp Animal extracts Peptides Food Science and Technology English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic Bighead carp
Animal extracts
Peptides
spellingShingle Bighead carp
Animal extracts
Peptides
Bakar, Jamilah
Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R
description Cathepsin D from the muscle of bighead carp (Aristichthys no bilis, R.)was extracted ,purified and partially characterized . The extract ion and purification o f the enzyme was achieved byautolysis of the muscle,acetone precipitation, gelfiltrat ion on Sephadex G100-120 and on ionexchange carboxymethyl cellulose (CMC)column chromatography . It had a molecular weight(m.w)of 37,500-38,000 dalton (D) with a pH optimum at 3.2 and temperature optimum of 50°C. Myofibril was also optimally digested at pH3.2. The purified enzyme had a single major peptideband on sodiumdodecyl sulfate polyacrylamide gelelectrophoresis SDS-PAGE )and was completely inhibited by pepstatin .
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Bakar, Jamilah
author_facet Bakar, Jamilah
author_sort Bakar, Jamilah
title Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R
title_short Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R
title_full Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R
title_fullStr Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R
title_full_unstemmed Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R
title_sort effect of storage on the changes in cathepsin d activity, nucleotide contents, peptide profiles and muscle ultrastructure of aristichthys nobilis, r
granting_institution Universiti Pertanian Malaysia
granting_department Food Science and Technology
publishDate 1993
url http://psasir.upm.edu.my/id/eprint/8357/1/FSMB_1993_1_A.pdf
_version_ 1747810787340058624

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