Lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae following intraperitoneal exposure to Javanese medaka (Oryzias javanicus, Bleeker 1854)

This study was conducted to determine the median lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus, Bleeker 1854) model, following intraperitoneal exposure. Javanese medaka was collected from estuary area of Su...

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Main Author:	Mastor, Siti Suhaiba
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Fishes - Diseases Bacterial diseases in fishes Streptococcus agalactiae
Online Access:	http://psasir.upm.edu.my/id/eprint/83581/1/FS%202018%20105%20-IR.pdf
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id	my-upm-ir.83581
record_format	uketd_dc
spelling	my-upm-ir.835812020-10-01T08:28:04Z Lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae following intraperitoneal exposure to Javanese medaka (Oryzias javanicus, Bleeker 1854) 2018-03 Mastor, Siti Suhaiba This study was conducted to determine the median lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus, Bleeker 1854) model, following intraperitoneal exposure. Javanese medaka was collected from estuary area of Sungai Pelek, Sepang, Selangor, and brought to the laboratory for quarantine and acclimatization. The fish were then challenged from 102 - 108 CFU/mlof virulent S. agalactiae via intraperitoneal injection. Mortalities and clinical signs were observed until 240 h post infection (hpi), while the dead fish were collected for bacterial isolation and histological analyses. Median lethal dose 50% (LD50) of S. agalactiae in Javanese medaka was determined at 5.3 × 102CFU/ml. Most of the infected fish showing lethargy, erratic swimming pattern, exophthalmia and necrosis at the injection site. The histopathological changes were mainly generalised congestion of the internal organs. Streptococcus agalactiae were successfully isolated from the dead fish. In the disease development studies, the number of Javanese medaka mortalities following infection by 103 CFU/ml of S. agalactiae was directly proportional with concentration of S. agalactiae in fish and severity of histopathological findings through 96 hpi. Clinical signs and histopathological assessment also showed that infected fish displayed similar findings compared to several the natural host of S. agalactiae. This study concluded that Javanese medaka was susceptible towards S. agalactiae infection and could be a potential alternative test organism for study of streptococcocis in fish. Fishes - Diseases Bacterial diseases in fishes Streptococcus agalactiae 2018-03 Thesis http://psasir.upm.edu.my/id/eprint/83581/ http://psasir.upm.edu.my/id/eprint/83581/1/FS%202018%20105%20-IR.pdf text en public masters Universiti Putra Malaysia Fishes - Diseases Bacterial diseases in fishes Streptococcus agalactiae Azmai, Mohammad Noor Amal
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Azmai, Mohammad Noor Amal
topic	Fishes - Diseases Bacterial diseases in fishes Streptococcus agalactiae
spellingShingle	Fishes - Diseases Bacterial diseases in fishes Streptococcus agalactiae Mastor, Siti Suhaiba Lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae following intraperitoneal exposure to Javanese medaka (Oryzias javanicus, Bleeker 1854)
description	This study was conducted to determine the median lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus, Bleeker 1854) model, following intraperitoneal exposure. Javanese medaka was collected from estuary area of Sungai Pelek, Sepang, Selangor, and brought to the laboratory for quarantine and acclimatization. The fish were then challenged from 102 - 108 CFU/mlof virulent S. agalactiae via intraperitoneal injection. Mortalities and clinical signs were observed until 240 h post infection (hpi), while the dead fish were collected for bacterial isolation and histological analyses. Median lethal dose 50% (LD50) of S. agalactiae in Javanese medaka was determined at 5.3 × 102CFU/ml. Most of the infected fish showing lethargy, erratic swimming pattern, exophthalmia and necrosis at the injection site. The histopathological changes were mainly generalised congestion of the internal organs. Streptococcus agalactiae were successfully isolated from the dead fish. In the disease development studies, the number of Javanese medaka mortalities following infection by 103 CFU/ml of S. agalactiae was directly proportional with concentration of S. agalactiae in fish and severity of histopathological findings through 96 hpi. Clinical signs and histopathological assessment also showed that infected fish displayed similar findings compared to several the natural host of S. agalactiae. This study concluded that Javanese medaka was susceptible towards S. agalactiae infection and could be a potential alternative test organism for study of streptococcocis in fish.
format	Thesis
qualification_level	Master's degree
author	Mastor, Siti Suhaiba
author_facet	Mastor, Siti Suhaiba
author_sort	Mastor, Siti Suhaiba
title	Lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae following intraperitoneal exposure to Javanese medaka (Oryzias javanicus, Bleeker 1854)
title_short	Lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae following intraperitoneal exposure to Javanese medaka (Oryzias javanicus, Bleeker 1854)
title_full	Lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae following intraperitoneal exposure to Javanese medaka (Oryzias javanicus, Bleeker 1854)
title_fullStr	Lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae following intraperitoneal exposure to Javanese medaka (Oryzias javanicus, Bleeker 1854)
title_full_unstemmed	Lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae following intraperitoneal exposure to Javanese medaka (Oryzias javanicus, Bleeker 1854)
title_sort	lethal dose, clinical signs, pathological changes and disease development of streptococcus agalactiae following intraperitoneal exposure to javanese medaka (oryzias javanicus, bleeker 1854)
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/83581/1/FS%202018%20105%20-IR.pdf
_version_	1747813398484090880

Lethal dose, clinical signs, pathological changes and disease development of Streptococcus agalactiae following intraperitoneal exposure to Javanese medaka (Oryzias javanicus, Bleeker 1854)

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