Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement
A citric acid producing yeast strain Saccharomycopsis lipolytic 5054 formerly known as Candida lipolytica was used throughout the study. The strain was obtained from Thailand Institute of Scientific and Technological Research (TISTR). A preliminary study on the development for the optimization of...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1994
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/8360/1/FSMB_1994_1_A.pdf |
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| Summary: | A citric acid producing yeast strain Saccharomycopsis lipolytic 5054 formerly known as Candida lipolytica was used throughout the study.
The strain was obtained from Thailand Institute of Scientific and
Technological Research (TISTR). A preliminary study on the development for
the optimization of the fermentation process was conducted. The experiments included the effect of medium contents such as CaC03 and initial glucose
concentrations, inoculum size, agitation rates, KLa (volumetric oxygen transfer
coefficient) and dissolved oxygen concentrations.
From the preliminary experiments, Medium I (l0% glucose, 0.1% yeast
extract, 0.1% MgS04.7H20, 0.2% NH4CI, 0.1% KH2PO, 1% CaC03, pH 6.6)
was selected as the fermentation medium throughout the study. The optimum
growth temperature and inoculum size was 30°C and 10%, respectively. 1%
CaC03 (w/v) was selected as the optimum concentration. The highest KLa
obtained was 29.6 hour-I at the agitation rate of 700 rpm using the Rushton
turbine impeller. This impeller speed was employed during the studies on
optimum dissolved oxygen concentration, where the highest citric acid
production obtained was 11.6 giL during fermentation at 50% (20 mg/L)
dissolved oxygen concentration. The optimum fermentation period was 50
hours where the exponential phase ended and the stationary phase started. |
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