Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement

A citric acid producing yeast strain Saccharomycopsis lipolytic 5054 formerly known as Candida lipolytica was used throughout the study. The strain was obtained from Thailand Institute of Scientific and Technological Research (TISTR). A preliminary study on the development for the optimization of...

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Main Author:	Osman, Rosmawati
Format:	Thesis
Language:	English English
Published:	1994
Subjects:	Candida lipolytica Citric acid Fermentation
Online Access:	http://psasir.upm.edu.my/id/eprint/8360/1/FSMB_1994_1_A.pdf
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spelling	my-upm-ir.83602012-11-02T04:14:49Z Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement 1994 Osman, Rosmawati A citric acid producing yeast strain Saccharomycopsis lipolytic 5054 formerly known as Candida lipolytica was used throughout the study. The strain was obtained from Thailand Institute of Scientific and Technological Research (TISTR). A preliminary study on the development for the optimization of the fermentation process was conducted. The experiments included the effect of medium contents such as CaC03 and initial glucose concentrations, inoculum size, agitation rates, KLa (volumetric oxygen transfer coefficient) and dissolved oxygen concentrations. From the preliminary experiments, Medium I (l0% glucose, 0.1% yeast extract, 0.1% MgS04.7H20, 0.2% NH4CI, 0.1% KH2PO, 1% CaC03, pH 6.6) was selected as the fermentation medium throughout the study. The optimum growth temperature and inoculum size was 30°C and 10%, respectively. 1% CaC03 (w/v) was selected as the optimum concentration. The highest KLa obtained was 29.6 hour-I at the agitation rate of 700 rpm using the Rushton turbine impeller. This impeller speed was employed during the studies on optimum dissolved oxygen concentration, where the highest citric acid production obtained was 11.6 giL during fermentation at 50% (20 mg/L) dissolved oxygen concentration. The optimum fermentation period was 50 hours where the exponential phase ended and the stationary phase started. Candida lipolytica Citric acid Fermentation 1994 Thesis http://psasir.upm.edu.my/id/eprint/8360/ http://psasir.upm.edu.my/id/eprint/8360/1/FSMB_1994_1_A.pdf application/pdf en public masters Universiti Pertanian Malaysia Candida lipolytica Citric acid Fermentation Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Candida lipolytica Citric acid Fermentation
spellingShingle	Candida lipolytica Citric acid Fermentation Osman, Rosmawati Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement
description	A citric acid producing yeast strain Saccharomycopsis lipolytic 5054 formerly known as Candida lipolytica was used throughout the study. The strain was obtained from Thailand Institute of Scientific and Technological Research (TISTR). A preliminary study on the development for the optimization of the fermentation process was conducted. The experiments included the effect of medium contents such as CaC03 and initial glucose concentrations, inoculum size, agitation rates, KLa (volumetric oxygen transfer coefficient) and dissolved oxygen concentrations. From the preliminary experiments, Medium I (l0% glucose, 0.1% yeast extract, 0.1% MgS04.7H20, 0.2% NH4CI, 0.1% KH2PO, 1% CaC03, pH 6.6) was selected as the fermentation medium throughout the study. The optimum growth temperature and inoculum size was 30°C and 10%, respectively. 1% CaC03 (w/v) was selected as the optimum concentration. The highest KLa obtained was 29.6 hour-I at the agitation rate of 700 rpm using the Rushton turbine impeller. This impeller speed was employed during the studies on optimum dissolved oxygen concentration, where the highest citric acid production obtained was 11.6 giL during fermentation at 50% (20 mg/L) dissolved oxygen concentration. The optimum fermentation period was 50 hours where the exponential phase ended and the stationary phase started.
format	Thesis
qualification_level	Master's degree
author	Osman, Rosmawati
author_facet	Osman, Rosmawati
author_sort	Osman, Rosmawati
title	Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement
title_short	Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement
title_full	Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement
title_fullStr	Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement
title_full_unstemmed	Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement
title_sort	citric acid production by saccharomycopsis lipolytica using batch culture: study on oxygen requirement
granting_institution	Universiti Pertanian Malaysia
granting_department	Food Science and Technology
publishDate	1994
url	http://psasir.upm.edu.my/id/eprint/8360/1/FSMB_1994_1_A.pdf
_version_	1747810788076158976

Citric Acid Production by Saccharomycopsis Lipolytica Using Batch Culture: Study on Oxygen Requirement

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