Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus

Protein A was produced extra cellularly by methicillinresistant Staphylococcus aureus, strain A676. A competitive- ELISA technique based on a competitive pinding between unlabelled protein A and conjugated protein A was employed to measure the concentration of protein A. A study was also conduct...

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Bibliographic Details
Main Author:	Tahir, Sharifah
Format:	Thesis
Language:	English English
Published:	1994
Subjects:	Staphylococcus aureus. Proteins. Methicillin Resistance.
Online Access:	http://psasir.upm.edu.my/id/eprint/8361/1/FSMB_1994_2_A.pdf
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id	my-upm-ir.8361
record_format	uketd_dc
spelling	my-upm-ir.83612012-06-29T08:56:09Z Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus 1994 Tahir, Sharifah Protein A was produced extra cellularly by methicillinresistant Staphylococcus aureus, strain A676. A competitive- ELISA technique based on a competitive pinding between unlabelled protein A and conjugated protein A was employed to measure the concentration of protein A. A study was also conducted to develop this technique. Rabbit IgG was first purified before it was used to coat a microtiterplate. IgG purified using ammonium sulphate precipitation in combination with DEAE-cellulose chromatography gave purer IgG than the caprylic acid method combined with saturated ammonium sulphate or single-step DEAE-cellulose ion exchange chromatography. The molecular weight of the purified IgG was 140,000 dalton based on SDS gelelectrophoresis. The minimum detectable amount of protein A was in the range of 0-20 ng/ml. The optimum concentration of IgG required for coating was 2pg/ml and the incubation time for the substrate was 20 minutes. Staphylococcus aureus. Proteins. Methicillin Resistance. 1994 Thesis http://psasir.upm.edu.my/id/eprint/8361/ http://psasir.upm.edu.my/id/eprint/8361/1/FSMB_1994_2_A.pdf application/pdf en public masters Universiti Pertanian Malaysia Staphylococcus aureus. Proteins. Methicillin Resistance. Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Staphylococcus aureus. Proteins. Methicillin Resistance.
spellingShingle	Staphylococcus aureus. Proteins. Methicillin Resistance. Tahir, Sharifah Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus
description	Protein A was produced extra cellularly by methicillinresistant Staphylococcus aureus, strain A676. A competitive- ELISA technique based on a competitive pinding between unlabelled protein A and conjugated protein A was employed to measure the concentration of protein A. A study was also conducted to develop this technique. Rabbit IgG was first purified before it was used to coat a microtiterplate. IgG purified using ammonium sulphate precipitation in combination with DEAE-cellulose chromatography gave purer IgG than the caprylic acid method combined with saturated ammonium sulphate or single-step DEAE-cellulose ion exchange chromatography. The molecular weight of the purified IgG was 140,000 dalton based on SDS gelelectrophoresis. The minimum detectable amount of protein A was in the range of 0-20 ng/ml. The optimum concentration of IgG required for coating was 2pg/ml and the incubation time for the substrate was 20 minutes.
format	Thesis
qualification_level	Master's degree
author	Tahir, Sharifah
author_facet	Tahir, Sharifah
author_sort	Tahir, Sharifah
title	Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus
title_short	Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus
title_full	Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus
title_fullStr	Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus
title_full_unstemmed	Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus
title_sort	production of extracellular protein a from a methicillin-resistant strain of staphylococcus aureus
granting_institution	Universiti Pertanian Malaysia
granting_department	Food Science and Technology
publishDate	1994
url	http://psasir.upm.edu.my/id/eprint/8361/1/FSMB_1994_2_A.pdf
_version_	1747810788317331456

Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus

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