Anti-obesity study of Elateriospermun tapos Blume hot aqueous extract in high-fat diet sprague-dawley rats

Obesity is a major public health burden and currently became a global pandemic. Current pharmacotherapy often accompanied with myriads of adverse side effects thus, the search for a safer novel cure is crucial. This study focus on the protective effects of Elateriospermum tapos extract on rats fe...

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Main Author:	Ja'afar, Nor Liyana
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Obesity - prevention & control
Online Access:	http://psasir.upm.edu.my/id/eprint/83626/1/FPSK%28m%29%202019%2027%20ir.pdf
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id	my-upm-ir.83626
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spelling	my-upm-ir.836262022-01-06T01:48:27Z Anti-obesity study of Elateriospermun tapos Blume hot aqueous extract in high-fat diet sprague-dawley rats 2018-04 Ja'afar, Nor Liyana Obesity is a major public health burden and currently became a global pandemic. Current pharmacotherapy often accompanied with myriads of adverse side effects thus, the search for a safer novel cure is crucial. This study focus on the protective effects of Elateriospermum tapos extract on rats fed with high-fat diet and its potential as an anti-obesity agent through inhibition of lipoprotein lipase (LPL) and triglyceride (TG) absorption. Thirty-six male Sprague-Dawley rats were allocated to negative control group (NC) fed with normal diet, while positive control (PC), drug control (DC), and treatment groups (T10, T100 and T200) were fed with high-fat and cafeteria diet. The DC group was given orlistat of 10mg/kg bodyweight and treatment groups were supplemented with 10, 100 and 200mg/kg bodyweight of E. tapos shell extract respectively. The bodyweight and caloric intake were measured weekly. After 4 weeks, the rats were euthanized and the plasma, liver and retroperitoneal white adipose tissues (RpWAT) were collected for biological assays of TG, LPL and plasma lipid profiles. Liver and adipose tissues were stained with Haematoxylin & Eosin for histological analysis. The PC group had significantly (p<0.05) higher in bodyweight, caloric intake and weight of RpWAT compared to NC group. The extract significantly (p<0.05) lowered the bodyweight and the weight of fat depots in treatment groups as compared to PC group. Nonetheless, the E. tapos shell extract supplementation has no positive inhibiting effects on the tissues TG and LPL level. Further, only T100 group had significantly (p<0.05) lowered plasma TG level as compared to PC. The size of both hepatocytes and adipocytes of supplemented groups were significantly (p<0.05) smaller as compared to those in obese group. Based on the findings, this study suggests that supplementation of E. tapos shell extract exhibited preventive effects on high-fat and cafeteria diet-induced obesity in rats. The extract however has no potential in the inhibition of TG and LPL level. Obesity - prevention & control 2018-04 Thesis http://psasir.upm.edu.my/id/eprint/83626/ http://psasir.upm.edu.my/id/eprint/83626/1/FPSK%28m%29%202019%2027%20ir.pdf text en public masters Universiti Putra Malaysia Obesity - prevention & control Bahari, Hasnah
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Bahari, Hasnah
topic	Obesity - prevention & control
spellingShingle	Obesity - prevention & control Ja'afar, Nor Liyana Anti-obesity study of Elateriospermun tapos Blume hot aqueous extract in high-fat diet sprague-dawley rats
description	Obesity is a major public health burden and currently became a global pandemic. Current pharmacotherapy often accompanied with myriads of adverse side effects thus, the search for a safer novel cure is crucial. This study focus on the protective effects of Elateriospermum tapos extract on rats fed with high-fat diet and its potential as an anti-obesity agent through inhibition of lipoprotein lipase (LPL) and triglyceride (TG) absorption. Thirty-six male Sprague-Dawley rats were allocated to negative control group (NC) fed with normal diet, while positive control (PC), drug control (DC), and treatment groups (T10, T100 and T200) were fed with high-fat and cafeteria diet. The DC group was given orlistat of 10mg/kg bodyweight and treatment groups were supplemented with 10, 100 and 200mg/kg bodyweight of E. tapos shell extract respectively. The bodyweight and caloric intake were measured weekly. After 4 weeks, the rats were euthanized and the plasma, liver and retroperitoneal white adipose tissues (RpWAT) were collected for biological assays of TG, LPL and plasma lipid profiles. Liver and adipose tissues were stained with Haematoxylin & Eosin for histological analysis. The PC group had significantly (p<0.05) higher in bodyweight, caloric intake and weight of RpWAT compared to NC group. The extract significantly (p<0.05) lowered the bodyweight and the weight of fat depots in treatment groups as compared to PC group. Nonetheless, the E. tapos shell extract supplementation has no positive inhibiting effects on the tissues TG and LPL level. Further, only T100 group had significantly (p<0.05) lowered plasma TG level as compared to PC. The size of both hepatocytes and adipocytes of supplemented groups were significantly (p<0.05) smaller as compared to those in obese group. Based on the findings, this study suggests that supplementation of E. tapos shell extract exhibited preventive effects on high-fat and cafeteria diet-induced obesity in rats. The extract however has no potential in the inhibition of TG and LPL level.
format	Thesis
qualification_level	Master's degree
author	Ja'afar, Nor Liyana
author_facet	Ja'afar, Nor Liyana
author_sort	Ja'afar, Nor Liyana
title	Anti-obesity study of Elateriospermun tapos Blume hot aqueous extract in high-fat diet sprague-dawley rats
title_short	Anti-obesity study of Elateriospermun tapos Blume hot aqueous extract in high-fat diet sprague-dawley rats
title_full	Anti-obesity study of Elateriospermun tapos Blume hot aqueous extract in high-fat diet sprague-dawley rats
title_fullStr	Anti-obesity study of Elateriospermun tapos Blume hot aqueous extract in high-fat diet sprague-dawley rats
title_full_unstemmed	Anti-obesity study of Elateriospermun tapos Blume hot aqueous extract in high-fat diet sprague-dawley rats
title_sort	anti-obesity study of elateriospermun tapos blume hot aqueous extract in high-fat diet sprague-dawley rats
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/83626/1/FPSK%28m%29%202019%2027%20ir.pdf
_version_	1747813403397718016

Anti-obesity study of Elateriospermun tapos Blume hot aqueous extract in high-fat diet sprague-dawley rats

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