Comparison of Javanese medaka (Oryzias javanicus, Bleeker, 1854) and zebrafish (Danio rerio, Hamilton, 1822) as a model organism for neurotoxicological study

Zebrafish is increasingly popular in neurotoxicology research due to their small size, low maintenance and efficient for high throughput testing. However, using nonnative species may raise concern on ecological aspect as accidental introduction of foreign species to our local aquatic environment...

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Main Author:	Ramlan, Nurul Farhana
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Neuroprotective agents Neurotoxicology
Online Access:	http://psasir.upm.edu.my/id/eprint/83648/1/FS%202019%2011%20-%20ir.pdf
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id	my-upm-ir.83648
record_format	uketd_dc
spelling	my-upm-ir.836482022-01-05T04:45:28Z Comparison of Javanese medaka (Oryzias javanicus, Bleeker, 1854) and zebrafish (Danio rerio, Hamilton, 1822) as a model organism for neurotoxicological study 2017-12 Ramlan, Nurul Farhana Zebrafish is increasingly popular in neurotoxicology research due to their small size, low maintenance and efficient for high throughput testing. However, using nonnative species may raise concern on ecological aspect as accidental introduction of foreign species to our local aquatic environment could cause ecosystem instability. Therefore, the applicability of using Javanese medaka (JM) in the neurotoxicology and developmental neurotoxicology (DNT) research will be assessed by using zebrafish as a reference model. Assessment of the exploratory, anxiety and social behaviour were conducted by using open field test and aquatic light/dark plus maze. Biochemical assessment was conducted using Fourier Transform Infrared (FTIR) analysis. Without exposure to neurotoxicants, JM displayed less exploratory behaviour and showed no difference for anxiety and social behaviour as compared to the zebrafish. JM exhibited light preference while zebrafish showed dark preference. Exposure to 1% ethanol resulted a reduction in exploratory behaviour, induction in anxiety like behaviour and no alteration in the social behaviour of JM. Meanwhile, zebrafish showed a reduction in anxiety like behaviour and no alteration in exploratory and social behaviour. Treatment with 1.6 μg/L endosulfan resulted increment in exploratory behaviour, induction in anxiety like behaviour and decrement in social behaviour for both fishes. Exposure to 100 mg/L caffeine resulted anxiogenic response and decrement in the exploratory behaviour for both fishes. Alteration in social behaviour was only observed in the zebrafish. Both fishes showed alteration in biochemical profile after exposure to ethanol or endosulfan with regard to different macromolecules in the brain tissues. This study also found that, JM is inefficient for DNT research as they are laborious, time-consuming and not amenable for high throughput screening. More studies need to be conducted to further develop JM as an ideal model organism for neurotoxicology research. Neuroprotective agents Neurotoxicology 2017-12 Thesis http://psasir.upm.edu.my/id/eprint/83648/ http://psasir.upm.edu.my/id/eprint/83648/1/FS%202019%2011%20-%20ir.pdf text en public masters Universiti Putra Malaysia Neuroprotective agents Neurotoxicology Wan Ibrahim, Wan Norhamidah
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Wan Ibrahim, Wan Norhamidah
topic	Neuroprotective agents Neurotoxicology
spellingShingle	Neuroprotective agents Neurotoxicology Ramlan, Nurul Farhana Comparison of Javanese medaka (Oryzias javanicus, Bleeker, 1854) and zebrafish (Danio rerio, Hamilton, 1822) as a model organism for neurotoxicological study
description	Zebrafish is increasingly popular in neurotoxicology research due to their small size, low maintenance and efficient for high throughput testing. However, using nonnative species may raise concern on ecological aspect as accidental introduction of foreign species to our local aquatic environment could cause ecosystem instability. Therefore, the applicability of using Javanese medaka (JM) in the neurotoxicology and developmental neurotoxicology (DNT) research will be assessed by using zebrafish as a reference model. Assessment of the exploratory, anxiety and social behaviour were conducted by using open field test and aquatic light/dark plus maze. Biochemical assessment was conducted using Fourier Transform Infrared (FTIR) analysis. Without exposure to neurotoxicants, JM displayed less exploratory behaviour and showed no difference for anxiety and social behaviour as compared to the zebrafish. JM exhibited light preference while zebrafish showed dark preference. Exposure to 1% ethanol resulted a reduction in exploratory behaviour, induction in anxiety like behaviour and no alteration in the social behaviour of JM. Meanwhile, zebrafish showed a reduction in anxiety like behaviour and no alteration in exploratory and social behaviour. Treatment with 1.6 μg/L endosulfan resulted increment in exploratory behaviour, induction in anxiety like behaviour and decrement in social behaviour for both fishes. Exposure to 100 mg/L caffeine resulted anxiogenic response and decrement in the exploratory behaviour for both fishes. Alteration in social behaviour was only observed in the zebrafish. Both fishes showed alteration in biochemical profile after exposure to ethanol or endosulfan with regard to different macromolecules in the brain tissues. This study also found that, JM is inefficient for DNT research as they are laborious, time-consuming and not amenable for high throughput screening. More studies need to be conducted to further develop JM as an ideal model organism for neurotoxicology research.
format	Thesis
qualification_level	Master's degree
author	Ramlan, Nurul Farhana
author_facet	Ramlan, Nurul Farhana
author_sort	Ramlan, Nurul Farhana
title	Comparison of Javanese medaka (Oryzias javanicus, Bleeker, 1854) and zebrafish (Danio rerio, Hamilton, 1822) as a model organism for neurotoxicological study
title_short	Comparison of Javanese medaka (Oryzias javanicus, Bleeker, 1854) and zebrafish (Danio rerio, Hamilton, 1822) as a model organism for neurotoxicological study
title_full	Comparison of Javanese medaka (Oryzias javanicus, Bleeker, 1854) and zebrafish (Danio rerio, Hamilton, 1822) as a model organism for neurotoxicological study
title_fullStr	Comparison of Javanese medaka (Oryzias javanicus, Bleeker, 1854) and zebrafish (Danio rerio, Hamilton, 1822) as a model organism for neurotoxicological study
title_full_unstemmed	Comparison of Javanese medaka (Oryzias javanicus, Bleeker, 1854) and zebrafish (Danio rerio, Hamilton, 1822) as a model organism for neurotoxicological study
title_sort	comparison of javanese medaka (oryzias javanicus, bleeker, 1854) and zebrafish (danio rerio, hamilton, 1822) as a model organism for neurotoxicological study
granting_institution	Universiti Putra Malaysia
publishDate	2017
url	http://psasir.upm.edu.my/id/eprint/83648/1/FS%202019%2011%20-%20ir.pdf
_version_	1747813405113188352

Comparison of Javanese medaka (Oryzias javanicus, Bleeker, 1854) and zebrafish (Danio rerio, Hamilton, 1822) as a model organism for neurotoxicological study

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