Transesterification of Palm Olein by Immobilised Microbial Lipases

Enzymatic transesterification of palm olein in organic solvent was studied. The enzyme was first immobilised to celite, lyophilised for 4 h and then added to a reaction medium composed of water-saturated hexane containing of 10% (w/v) palm olein. The catalytic performance of the enzyme was evalua...

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Main Author:	Sidek, Hamidah
Format:	Thesis
Language:	English English
Published:	1996
Subjects:	Transesterification. Immobilized enzymes.
Online Access:	http://psasir.upm.edu.my/id/eprint/8367/1/FSMB_1996_3_A.pdf
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id	my-upm-ir.8367
record_format	uketd_dc
spelling	my-upm-ir.83672012-07-02T02:35:01Z Transesterification of Palm Olein by Immobilised Microbial Lipases 1996 Sidek, Hamidah Enzymatic transesterification of palm olein in organic solvent was studied. The enzyme was first immobilised to celite, lyophilised for 4 h and then added to a reaction medium composed of water-saturated hexane containing of 10% (w/v) palm olein. The catalytic performance of the enzyme was evaluated by determining changes in the triglycerides (TG) composition and concentration by reverse-phase high performance liquid chromatography (RP-HPLC) and the formation of free fatty acids (FF A) by titration, respectively. For Candida rugosa lipase, the optimal water content was controlled by lyophilisation of the lipase preparation for 4 h. The addition of water to the dried immobilised preparation shifted the reaction equilibrium to favour net hydrolysis. Of the commercially available lipases that were investigated, lipases from Pseudomonas sp. and the lipase from Rhizomucor miehei resulted in the highest extent of transesterification. Besides palm olein, palm kernel olein and coconut oil showed some changes in the triglyceride composition after transesterification process. Changes in the palm olein concentration in the range 5- 1 00% increased the degree of transesterification of the immobilised Pseudomonas sp. and declined with the R. miehei lipase. The maximum enzyme activity was reached at an enzyme loading of 0.40% (w/w). The optimum temperature for trans esterification by immobilised Pseudomonas sp. lipase was 48°C. Hexane, cyclohexane and isooctane were found to be particularly useful organic solvents in the transesterification process. Water-saturated hexane system can be replaced by either dimethysulfoxide- or dimethylformamide-saturated hexane. Methanol was not suitable for the transesterification process. In all cases, the transesterification process resulted in the formation of PPP (tripalmitin), a trisaturated triglyceride initially undetected in the oil, and minor increases in the concentration of 000, OOL, OLL, SOS, where P, 0, L and S are palmitic, oleic, linoleic and stearic acid, respectively thus increasing the slip melting point of the final product. Transesterification. Immobilized enzymes. 1996 Thesis http://psasir.upm.edu.my/id/eprint/8367/ http://psasir.upm.edu.my/id/eprint/8367/1/FSMB_1996_3_A.pdf application/pdf en public masters Universiti Pertanian Malaysia Transesterification. Immobilized enzymes. Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Transesterification. Immobilized enzymes.
spellingShingle	Transesterification. Immobilized enzymes. Sidek, Hamidah Transesterification of Palm Olein by Immobilised Microbial Lipases
description	Enzymatic transesterification of palm olein in organic solvent was studied. The enzyme was first immobilised to celite, lyophilised for 4 h and then added to a reaction medium composed of water-saturated hexane containing of 10% (w/v) palm olein. The catalytic performance of the enzyme was evaluated by determining changes in the triglycerides (TG) composition and concentration by reverse-phase high performance liquid chromatography (RP-HPLC) and the formation of free fatty acids (FF A) by titration, respectively. For Candida rugosa lipase, the optimal water content was controlled by lyophilisation of the lipase preparation for 4 h. The addition of water to the dried immobilised preparation shifted the reaction equilibrium to favour net hydrolysis. Of the commercially available lipases that were investigated, lipases from Pseudomonas sp. and the lipase from Rhizomucor miehei resulted in the highest extent of transesterification. Besides palm olein, palm kernel olein and coconut oil showed some changes in the triglyceride composition after transesterification process. Changes in the palm olein concentration in the range 5- 1 00% increased the degree of transesterification of the immobilised Pseudomonas sp. and declined with the R. miehei lipase. The maximum enzyme activity was reached at an enzyme loading of 0.40% (w/w). The optimum temperature for trans esterification by immobilised Pseudomonas sp. lipase was 48°C. Hexane, cyclohexane and isooctane were found to be particularly useful organic solvents in the transesterification process. Water-saturated hexane system can be replaced by either dimethysulfoxide- or dimethylformamide-saturated hexane. Methanol was not suitable for the transesterification process. In all cases, the transesterification process resulted in the formation of PPP (tripalmitin), a trisaturated triglyceride initially undetected in the oil, and minor increases in the concentration of 000, OOL, OLL, SOS, where P, 0, L and S are palmitic, oleic, linoleic and stearic acid, respectively thus increasing the slip melting point of the final product.
format	Thesis
qualification_level	Master's degree
author	Sidek, Hamidah
author_facet	Sidek, Hamidah
author_sort	Sidek, Hamidah
title	Transesterification of Palm Olein by Immobilised Microbial Lipases
title_short	Transesterification of Palm Olein by Immobilised Microbial Lipases
title_full	Transesterification of Palm Olein by Immobilised Microbial Lipases
title_fullStr	Transesterification of Palm Olein by Immobilised Microbial Lipases
title_full_unstemmed	Transesterification of Palm Olein by Immobilised Microbial Lipases
title_sort	transesterification of palm olein by immobilised microbial lipases
granting_institution	Universiti Pertanian Malaysia
granting_department	Food Science and Technology
publishDate	1996
url	http://psasir.upm.edu.my/id/eprint/8367/1/FSMB_1996_3_A.pdf
_version_	1747810789790580736

Transesterification of Palm Olein by Immobilised Microbial Lipases

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